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細(xì)胞代謝、細(xì)胞凋亡、細(xì)胞增殖、細(xì)胞氧化應(yīng)激、細(xì)胞損傷與修復(fù)、細(xì)胞活力、遷移、侵襲、趨化、干細(xì)胞
| 產(chǎn)品英文名稱 | Anti-GAPDH Rabbit Polyclonal Antibody |
| 產(chǎn)品中文名稱 | GAPDH兔多克隆抗體 |
| 別名 | GAPDH; GAPD; CDABP0047; OK/SW-cl.12; Glyceraldehyde-3-phosphate dehydrogenase; GAPDH; Peptidyl-cysteine S-nitrosylase GAPDH |
| 克隆類型 | 多克隆 |
| 檢測類型 | WB,IHC-P |
| 反應(yīng)性 | 人,小鼠,雞,兔,猴,大鼠,綿羊 |
| 宿主 | 兔 |
| 濃度 | 1 mg/ml |
| 免疫原 | 重組蛋白 |
| 產(chǎn)品形式 | 液體形式 |
| 基因ID | 2597 |
| 目標(biāo)條帶分子量 | 37 |
| 蛋白序列鏈接 | /uniprot/P04406 |
| 存儲(chǔ)緩沖液 | PBS緩沖液（pH 7.4），含有0.5%BSA（穩(wěn)定劑），0.02％（防腐劑）和50％甘油。 |
| 保存建議 | 自發(fā)貨之日起，-20°C可穩(wěn)定保存1年。為限度的避免損失，請(qǐng)?jiān)诖蜷_管蓋之前融化抗體并離心。我們建議使用前分裝以避免反復(fù)凍融。 |
| 運(yùn)輸條件 | 冰袋運(yùn)輸（藍(lán)冰） |
| 警告 | 本文列出的產(chǎn)品僅供研究使用，不適用于人類或臨床診斷。我們產(chǎn)品所推薦應(yīng)用，不是建議使用我們的產(chǎn)品去違反任何或許可證。對(duì)于使用本產(chǎn)品可能發(fā)生的侵權(quán)或其他違規(guī)行為，我們不承擔(dān)任何責(zé)任。 |
| 其他 | 該抗體可檢測內(nèi)源蛋白 |
| 背景 | GAPDH encodes a member of the glyceraldehyde-3-phosphate dehydrogenase protein family. Glyceraldehyde-3-phosphate dehydrogenase has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. The product of GAPDH catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). Glyceraldehyde-3-phosphate dehydrogenase has additionally been identified to have uracil DNA glycosylase activity in the nucleus. Also, Glyceraldehyde-3-phosphate dehydrogenase contains a peptide that has antimicrobial activity against E. coli, P. aeruginosa, and C. albicans. Studies of a similar protein in mouse have assigned a variety of additional functions including nitrosylation of nuclear proteins, the regulation of mRNA stability, and acting as a transferrin receptor on the cell surface of macrophage. Many pseudogenes similar to this locus are present in the human genome. Alternative splicing results in multiple transcript variants. |
Fig. Western blot analysis of 293T (1， Rat brain (2， NIH 3T3 (3， Sheep Muscle (4， 兔 testis (5， diluted at 1:20000. Secondary antibody was diluted at 1:20000.
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