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KitContents
Please refer to complete instructions manual supplied with the product

SpeciesReactivity
Human

Form
Stds=0-25 ng/ml; Sample=50 ul; Sensitivity= 0.05 ng/ml; 75 min assay; Quantitative; Competitive assay; 96 tests

Samples
Serum. Other fluids not optimized.

SpCrossreactivity
The following compounds were tested for cross-reactivity with the Direct Pregnenolone ELISA kit with pregnenolone cross-reacting at 100%: Pregnenolone (100%), Progesterone (6%), Dehydroisoandrosterone (5.2%), 5a-Androstandiol (4.7%). Epiandrosterone, Pregnenolone Sulfate, Androstandione, 5a-Androsterone, DHEAS, Etiocholanolone were <1%. The following steroids were tested but cross-reacted at less than 0.1%: Adrenosterone, Aldosterone, Androstenedione, Cholesterol, Corticosterone, 5a-DHT, 17ß-Estradiol, Estriol and Testosterone. Pregnolonekit has been designed and tested for human serum samples. It may be optimized for other biological fluids. It has not been tested in animals (rat, mouse, etc). Since the steroid hormone is the same in all species, this kit should work in most species as long as the sample cocn is within the range of this kit.

MSDS
TMB (substrate), H2SO4 (1% in a buffered stop solution), and Prolcin-300 (0.1% v/v in standards, sample diluent and HRP-conjugates

For research use only, not for diagnostic or therapeutic use.