您好, 歡迎來到化工儀器網(wǎng)！ 登錄| 免費注冊| 產(chǎn)品展廳| 收藏商鋪|
您好, 歡迎來到化工儀器網(wǎng)！ 登錄| 免費注冊| 產(chǎn)品展廳| 收藏商鋪|
18906011628
重組溶葡萄球菌素/Lysostaphin
| Synonyms | Lysostaphin, EC 3.4.24.75, Glycyl-glycine endopeptidase. |
| Description | Lysostaphin, an endopeptidase specific for the cell wall peptidoglycan of staphylococci, is an extremely potent anti-staphylococcal agent. Lysostaphin is used as a research and diagnostic tool. Because it lyses staphylococci efficiently, it is widely used when preparing staphylococcal DNA or other cellular components for genetic and biochemical studies and for the preparation of protoplasts for transformation. Preparation and analysis of bacterial DNA has become a powerful tool used by clinical and other microbiologists in epidemiological studies aimed at tracing sources of infection or bacterial contamination. The Mw of lysostaphin is 26,921 (Recsei et al, PNAS 1987). |
| Source | Escherichia Coli. |
| Physical Appearance | Sterile Filtered lyophilized powder. |
| Formulation | The protein was lyophilized without any additives. |
| Stability | Lysostaphin although stable at 4°C for 6 months, should be stored desiccated below -18°C. Please prevent freeze-thaw cycles. Lysostaphin has optimal stability in the range of pH 4.5, and optimal activity in the range of pH 8. For a stock solution it is recommended to work with 10mg/ml lysostaphin in 10mM sodium acetate pH 4.5. |
| Solubility | It is recommended to reconstitute the lyophilized Lysostaphin in 20mM sodium acetate, pH 4.5, which can then be further diluted to other aqueous solutions. |
| Purity | 96.9% as determined by RP-HPLC. |
| Biological Activity | Determined by the decrease in turbidity of a suspension of heat-killed Staphylococcus aureus at pH8.0, 30°C. For a reaction buffer it is recommended to work with 200mM Tris-HCl pH 8. |
| Specific Activity | Determined to be 4,243 units/mg. |
| Protein content | Protein quantitation was carried out by two independent methods 1. UV spectroscopy at 280 nm using the absorbency value of 2.02 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (InliGenetics). 2. Analysis RP-HPLC, using a calibrated solution of Lysostaphin as a Reference Standard. |
| Usage | Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. |
請輸入賬號
請輸入密碼
請輸驗證碼
以上信息由企業(yè)自行提供，信息內(nèi)容的真實性、準確性和合法性由相關(guān)企業(yè)負責(zé)，化工儀器網(wǎng)對此不承擔(dān)任何保證責(zé)任。
溫馨提示：為規(guī)避購買風(fēng)險，建議您在購買產(chǎn)品前務(wù)必確認供應(yīng)商資質(zhì)及產(chǎn)品質(zhì)量。
