目錄:北京索萊寶科技有限公司>>生化試劑>>堿基、核酸及衍生物>> D8020Deoxyribonucleic acid sodium salt from calf thymus
Deoxyribonucleic acid sodium salt from calf thymus
貨號：D8020
規(guī)格：50mg/支
小牛胸腺DNA(CT-DNA)是一種天然的DNA廣泛用于研究DNA綁定抗癌劑和DNA綁定代理,調(diào)節(jié)DNA結(jié)構(gòu)和功能。
小牛胸腺DNA的DNA也被運(yùn)用于物理化學(xué)研究行為的解決方案。 溶解性：溶于水，參考濃度1mg/ml，4℃條件下輕輕搖晃為佳方法，但不可劇烈震蕩。
Preparation Note
This product is extracted using a method that causes shearing yielding a highly polymerized mixture of double and single stranded DNA . However, double stranded DNA is the predominant form.
This product is prepared from calf thymus tissue of unspecified gender.
Quality
"Highly Polymerized"
Reconstitution
Solutions of DNA have been stored successfully for several months at 4 C, pH 7.5-8, in 10 mM Tris with 1 mM EDTA and without a bacteriostatic agent. At low concentrations (μg/ml) DNA tends to absorb onto the surfaces of plastic tubes. It is not recommended to store DNA in highly alkaline solutions since DNA tends to degrade at alkaline pH greater than 8.0.
This product can be dissolved at 2 mg/ml in water. To reduce further shearing of the DNA, no sonication or stirring should be used. Gentle inversion overnight at 0-4 °C is recommended to compley solubilize the DNA. The presence of 1 mM EDTA is recommended to prevent nucleases from degrading the DNA.
Physical form
fibrous preparation
Application
Calf thymus DNA (CT-DNA) is a natural DNA widely used in studies of DNA binding anticancer agents and DNA binding agents that modulate DNA structure and function. Calf thymus DNA is also used in physicochemical studies of DNA behavior in solution.
Physical properties
λmax 259 nm (100 mM phosphate buffer, pH 7.0).
DNA from calf thymus is 41.9 mole % G-C and 58.1 mole % A-T.1 An OD of 1.0 at 260 nm corresponds to ~ 50 μg of double-stranded DNA.
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