欧美……一区二区三区,欧美日韩亚洲另类视频,亚洲国产欧美日韩中字,日本一区二区三区dvd视频在线

上海復(fù)祥生物科技有限公司

當(dāng)前位置:上海復(fù)祥生物科技有限公司>>細(xì)胞庫>>人正常細(xì)胞>>HUV-EC-C人臍靜脈內(nèi)皮細(xì)胞

人臍靜脈內(nèi)皮細(xì)胞

參   考   價(jià): ¥ 1

訂  貨  量: ≥1 件

具體成交價(jià)以合同協(xié)議為準(zhǔn)

產(chǎn)品型號(hào):HUV-EC-C

品       牌:其他品牌

廠商性質(zhì):生產(chǎn)商

所  在  地:上海市

更新時(shí)間:2024-11-12 20:48:36瀏覽次數(shù):2743

聯(lián)系我時(shí),請(qǐng)告知來自 化工儀器網(wǎng)
供貨周期 一周 規(guī)格 T25
貨號(hào) HUV-EC-C 應(yīng)用領(lǐng)域 醫(yī)療衛(wèi)生,化工,生物產(chǎn)業(yè),制藥
主要用途 普通細(xì)胞株-科研實(shí)驗(yàn)
CRL-1730 HUV-EC-C 人臍靜脈內(nèi)皮細(xì)胞,
ATCC 細(xì)胞|細(xì)胞系|細(xì)胞株|腫瘤細(xì)胞|細(xì)胞|貼壁細(xì)胞|懸浮細(xì)胞|,
細(xì)胞庫管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和培養(yǎng)條件

CRL-1730 HUV-EC-C 人臍靜脈內(nèi)皮細(xì)胞

ATCC® Number: CRL-1730™ Price: $280.00

Designations: HUV-EC-C

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens (human)

Morphology: endothelial

CRL-1730 HUV-EC-C 人臍靜脈內(nèi)皮細(xì)胞

Source: Organ: umbilical vein

Tissue: vascular endothelium

Disease: normal

Cell Type: endothelial

Cellular Products: factor VIII [23284]

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.


Applications: transfection host (technology from amaxa)

Tumorigenic: No

DNA Profile (STR): Amelogenin: X

CSF1PO: 11,12

D13S317: 9,11

D16S539: 11,12

D5S818: 11,12

D7S820: 8,12

THO1: 6,9.3

TPOX: 8,11

vWA: 16

Cytogenetic Analysis: This is a hypodiploid human cell line. The modal chromosome number

was 45 occurring in 72% of cells counted. The rate of polyploid cells was 15.8%. All cells had monosomic N13 and the subclone with additional monosomic N15 predominates. Other coexisting subclones include those with 46,XX,-11,-13,i(11p),i(11q) and 46,XX,+11,-13 karyotypes. Both X chromosomes appear normal.

Comments: Endothelial Cell Growth Supplement (ECGS) and unidentified factors from bovine pituitary, hypothalamus or whole brain extracts are mitogenic for this line. [23284]

The cells have a life expectancy of 50 to 60 population doublings.

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated of F-12K Medium, Catalog No. 30-2004. To make the complete growth medium, add the following components to the base medium: 0.1 mg/ml heparin; 0.03-0.05 mg/ml endothelial cell growth supplement (ECGS); adjust to a final concentration of 10% fetal bovine serum.

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Temperature: 37.0°C

Subculturing: Protocol:

1.Remove and discard culture medium.

2.Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.

3.Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.

4.Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

5.Add appropriate aliquots of the cell suspension to new culture vessels.

6.Incubate cultures at 37°C.

Subc*tion Ratio: A subc*tion ratio of 1:2 to 1:3 is recommended

Medium Renewal: Two to three times per week

Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO

Storage temperature: liquid nitrogen vapor phase

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2004

recommended serum:ATCC 30-2020

References: 23284: Hoshi H, McKeehan WL. Brain- and liver cell-derived factors are required for growth of human endothelial cells in serum-free culture. Proc. Natl. Acad. Sci. USA 81: 6413-6417, 1984. PubMed: 6333682

29192: Zahedi K. Characterization of the binding of serum amyloid P to laminin. J. Biol. Chem. 272: 2143-2148, 1997. PubMed: 8999915

33021: Soker S, et al. Characterization of novel vascular endothelial growth factor (VEGF) receptors on tumor cells that bind VEGF165 via its exon 7-endoded domain. J. Biol. Chem. 271: 5761-5767, 1996. PubMed: 8621443



CRL-1730 HUV-EC-C
























HK-2 人腎近曲小管上皮細(xì)胞

CRL-2190 HK-2 人腎近曲小管上皮細(xì)胞,

人肺成纖維細(xì)胞

CCL-153 HFL1 人肺成纖維細(xì)胞,原代細(xì)胞

人肝星形細(xì)胞

LX-2 人肝星形細(xì)胞,$r$nATCC 細(xì)胞|細(xì)

會(huì)員登錄

X

請(qǐng)輸入賬號(hào)

請(qǐng)輸入密碼

=

請(qǐng)輸驗(yàn)證碼

收藏該商鋪

X
該信息已收藏!
標(biāo)簽:
保存成功

(空格分隔,最多3個(gè),單個(gè)標(biāo)簽最多10個(gè)字符)

常用:

提示

X
您的留言已提交成功!我們將在第一時(shí)間回復(fù)您~

以上信息由企業(yè)自行提供,信息內(nèi)容的真實(shí)性、準(zhǔn)確性和合法性由相關(guān)企業(yè)負(fù)責(zé),化工儀器網(wǎng)對(duì)此不承擔(dān)任何保證責(zé)任。

溫馨提示:為規(guī)避購買風(fēng)險(xiǎn),建議您在購買產(chǎn)品前務(wù)必確認(rèn)供應(yīng)商資質(zhì)及產(chǎn)品質(zhì)量。

撥打電話
在線留言
激情字幕久久久字幕中文-一区二区三区免费黄片| 日韩欧美国产综合久久-国产精品一起草在线观看| 亚洲av综合av一区东京热-黄页免费视频网站在线观看| 国产免费一区二区三区不-日本少妇免费一区二区三区| 黄片黄片在线免费观看-激情综合网激情五月俺也去| 国内精产熟女自线一二三区-六月丁香婷婷在线观看| 中文字幕偷拍亚洲九色-亚洲视频不卡一区二区天堂| 亚洲欧美激情自拍色图-国产亚洲精品sese在线播放| 中文字幕日韩精品不卡在线一区-国产tv日韩在线观看视频| 亚洲精品激情一区二区-久久成人国产欧美精品一区二区| 熟女少妇免费一区二区-麻豆一区二区三区免费在线观看| 狠狠狠狠爱精品一二三四区-l舌熟女av国产精品| 欧美日韩成人在线观看-久久五月婷婷免费视频| 亚洲女人黄色录像一区-日韩av电影在线免费看| 欧美黄色三级视频网站-国产九九热视频在线观看| 久久网站中文字幕精品-三级精品久久中文字幕| 91蜜桃传媒一二三区-日韩欧美国产一区呦呦| 人妻日韩精品中文字幕图片-麻豆极度性感诱人在线露脸| 久久网站中文字幕精品-三级精品久久中文字幕| 国产精品一区二区在线免费-久久精品国产亚洲av热明星| 青青草原免费国产在线视频-精品人妻乱码一区二区三区四区| 久久精品人妻一区二区三区极品-久久99热这里只有精品免费| 日本中文字幕永久在线人妻蜜臀-欧美一区二区的网站在线观看| 深夜三级福利在线播放-日韩精品一区二区在线天天狠天| 亚洲欧美日韩国产一区二区三区-国产欧美日韩一区二区免费| 欧美日本亚一级二级三区久久精品-日韩欧美一区二区久久婷婷| 国产精品美女在线网址-久草免费福利在线观看视频| 精彩亚洲一区二区三区-中文字幕中文字幕在线色站| 精彩亚洲一区二区三区-中文字幕中文字幕在线色站| 日韩综合精品一区二区-丝袜美腿熟女人妻经典三级| 日韩精品人妻系列一区-亚洲女同性一区二区三区| 欧美精品一区二区三区爽爽爽-日韩国产精品亚洲经典| 亚洲综合久久综合激情-日韩欧美精品人妻二区少妇| 91九色蝌蚪丝袜人妻-国产精品9999网站| 天天日天天干天天综合-99久久综合狠狠综合久久| 五月婷婷六月在线观看视频-亚洲黑寡妇黄色一级片| 日本亚洲精品中字幕日产2020-很黄很黄的裸交视频网站| 中文字幕日韩不卡久久-五月天中文字幕啊av| 欧美精品一区二区三区爽爽爽-日韩国产精品亚洲经典| 亚洲综合av一区二区三区-高潮又爽又黄无遮挡激情视频| 亚洲视频一区二区三区免费-国产一级黄色大片在线|