CCL-120 CCRF-SD 人外周血淋巴細(xì)胞
ATCC® Number: CCL-120™ Price: $338.00
Designations: CCRF-SB [CCRF SB]
Depositors: GE Foley
Biosafety Level: 2 [Cells Contain HERPESVIRUS ]
Shipped
: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Homo sapiens (human
)
Morphology: lymphoblast
CCL-120 CCRF-SD 人外周血淋巴細(xì)胞
Source: Organ: peripheral blood
Disease: acute lymphoblastic leukemia
Cell Type: B lymphoblast;
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Antigen Expression: HLA A1, A2, B12, B17, Cw2
DNA Profile (STR): Amelogenin: X,Y
CSF1PO: 10,12
D13S317: 10,12
D16S539: 9,13
D5S818: 11,12
D7S820: 11,12
THO1: 9,10
TPOX: 8
vWA: 18
Isoenzymes: G6PD, B
Age: 11.5 years
Gender: male
Ethnicity: Caucasian
Comments: A T cell line (HSB-2, ATCC CCL-120.1) was also obtained from the same buffy coat preparation.
Derived from a buffy coat preparation from a patient with ALL.
The cells are EBNA positive.
Propagation: ATCC complete growth medium: RPMI 1640 medium, 90%; fetal bovine serum, 10% - OR - Iscove's modified Dulbecco's medium, 90%; fetal bovine serum, 10%
Temperature: 37.0°C
Subculturing: Protocol: Cultures can be maintained by addition or replacement of medium. Establish new cultures at 3 X 10(5) viable cells/ml.
Medium Renewal: Add fresh medium (10% to 20% by volume) every 2 to 3 days
Related Products: derived from same individual:ATCC CCL-120.1
References: 24413: . M.D. Anderson Hospital and Tumor Inst. Monograph. 21: ; 1967.
25967: Adams RA. Formal discussion: the role of transplantation in the experimental investigation of human leukemia and lymphoma. Cancer Res. 27: 2479-2482, 1967. PubMed: 4170381
CCL-120 CCRF-SD