欧美……一区二区三区,欧美日韩亚洲另类视频,亚洲国产欧美日韩中字,日本一区二区三区dvd视频在线

上海復(fù)祥生物科技有限公司

當(dāng)前位置:上海復(fù)祥生物科技有限公司>>細(xì)胞庫>>小鼠正常細(xì)胞>>CRL-2279MS1 小鼠胰島內(nèi)皮細(xì)胞

MS1 小鼠胰島內(nèi)皮細(xì)胞

參   考   價:面議
具體成交價以合同協(xié)議為準(zhǔn)

產(chǎn)品型號:CRL-2279

品       牌:ATCC

廠商性質(zhì):生產(chǎn)商

所  在  地:上海市

更新時間:2024-11-14 20:35:18瀏覽次數(shù):2221

聯(lián)系我時,請告知來自 化工儀器網(wǎng)
供貨周期 一周 規(guī)格 T25
貨號 MS1 應(yīng)用領(lǐng)域 醫(yī)療衛(wèi)生,化工,生物產(chǎn)業(yè),制藥
主要用途 科學(xué)研究
CRL-2279 MS1 小鼠胰島內(nèi)皮細(xì)胞,原代細(xì)胞|細(xì)胞系|細(xì)胞株|菌種;細(xì)胞庫管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和培養(yǎng)條件!

CRL-2279 MS1 小鼠胰島內(nèi)皮細(xì)胞 的詳細(xì)介紹

CRL-2279 MS1 小鼠胰島內(nèi)皮細(xì)胞

ATCC® Number:  CRL-2279™

Designations:  MS1 (MILE SVEN 1)

Depositors:   JL Arbiser

Biosafety Level: 2 [CELLS CONTAIN PAPOVAVIRUS ]

Shipped:  froz


Organism: Mus musculus (mouse)en

Medium & Serum:  See Propagation

Growth Properties: adherent

Morphology: endothelial


CRL-2279

Source: Organ: pancreas

Strain: C57BL/6

Tissue: islet of Langerhans; endothelium

Cell Type: SV40 transformed

Cellular Products: tissue inhibitor of bioreactive matrix metalloproteinase (high levels)

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.


Receptors: vascular endothelial growth factor (VEGF), expressed

Comments: MS1 is a pancreatic islet endothelial cell line established in 1994.

Primary islet endothelial cells were transduced with a temperature sensitive SV40 large T antigen (tsA-58-3) constructed and screened for resistance to G418.

Resistant colonies were isolated in cloning rings and screened for uptake of diI-Ac-LDL.

The line retains many properties of endothelial cells including uptake of acetylated LDL and expression of both Factor VIII related antigen and VEGF receptor.

The cells are useful for the study of signal transduction of angigenic factors.

It is the only line known that can give rise to benign hemangiomas.

Propagation:  ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 5%.

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Temperature: 37.0°C

Subculturing:  Protocol:

Remove and discard culture medium.

Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.

Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.

Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

Add appropriate aliquots of the cell suspension to new culture vessels.

Incubate cultures at 37°C.

Subc*tion Ratio: A subc*tion ratio of 1:4 to 1:8 is recommended

Medium Renewal: 2 to 3 times per week

Preservation:  Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO

Storage temperature: liquid nitrogen vapor phase

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002

recommended serum:ATCC 30-2020

References: 22603: Arbiser JL, et al. Oncogenic H-ras stimulates tumor angiogenesis by two distinct pathways. Proc. Natl. Acad. Sci. USA 94: 861-866, 1997. PubMed: 9023347

51452: Arbiser JL, et al. Overexpression of VEGF 121 in immortalized endothelial cells causes conversion to slowly growing angiosarcoma and high level expression of the VEGF receptors VEGFR-1 and VEGFR-2 in vivo. Am. J. Pathol. 156: 1469-1476, 2000. PubMed: 10751370


















NCTC 1469 小鼠正常肝

CCL-9.1 NCTC 1469 小鼠正常肝細(xì)胞

HT22 小鼠海馬神經(jīng)元細(xì)胞系

HT22 小鼠海馬神經(jīng)元細(xì)胞系$r$n上海復(fù)祥生物

TC-1 小鼠肺上皮細(xì)胞

TC-1 小鼠肺上皮細(xì)胞$r$n上海復(fù)祥生物提供

會員登錄

X

請輸入賬號

請輸入密碼

=

請輸驗(yàn)證碼

收藏該商鋪

X
該信息已收藏!
標(biāo)簽:
保存成功

(空格分隔,最多3個,單個標(biāo)簽最多10個字符)

常用:

提示

X
您的留言已提交成功!我們將在第一時間回復(fù)您~

以上信息由企業(yè)自行提供,信息內(nèi)容的真實(shí)性、準(zhǔn)確性和合法性由相關(guān)企業(yè)負(fù)責(zé),化工儀器網(wǎng)對此不承擔(dān)任何保證責(zé)任。

溫馨提示:為規(guī)避購買風(fēng)險,建議您在購買產(chǎn)品前務(wù)必確認(rèn)供應(yīng)商資質(zhì)及產(chǎn)品質(zhì)量。

撥打電話
在線留言
婷婷亚洲欧美综合丁香亚洲-超刺激国语对白在线视频| 亚洲精品一区网站在线观看-黄页视频免费观看网站| 看肥婆女人黄色儿逼视频-秋霞电影一区二区三区四区| 欧美精品一区二区不卡-精品国产一区二区三区香蕉网址| 亚洲另类自拍唯美另类-99国产精品兔免久久| 三上悠亚免费观看在线-青青草原在线视频观看精品| 日韩精品一区二区三区十八-日韩人妻少妇一区二区三区| 国产成人精品亚洲精品密奴-国产成人AV无码精品| 国产欧美日本一区二区-一区二区三区亚洲在线播放| 成人免费资源在线观看-欧美国产日韩高清在线综合| 精品少妇一区二区18-一区二区三区日韩在线播放| 亚洲欧美另类综合偷拍-婷婷社区综合在线观看| hd在线观看一区二区-免费一区二区三区毛片在线| 精彩亚洲一区二区三区-中文字幕中文字幕在线色站| 99在线观看精品视频免费-国产极品一区二区三区四区| 日本中文字幕啊啊啊啊-久久精品伊人久久精品伊人| 国产在线不卡高清一区-日本一区二区三区四区无卡| 久久影视av一区二区-人妻激情乱偷一区二区三区| 国产午夜精品理论片A级漫画-久久精品国产99亚洲精品| 国产aa视频一区二区三区-国产精品久久久久久久毛片动漫| 中文字幕日韩精品不卡在线一区-国产tv日韩在线观看视频| 久久久国产精品电影片-精品孕妇人妻一区二区三区| 亚洲黄片三级三级三级-国产成人一区二区在线视频| 国产一区二区无套内射-国内精品久久久久久久齐pp| 97人妻精品一区二区三区爱与-日韩精品亚洲专区在线观看| 免费av一区在线观看-国产精品视频高潮流白浆视频免费| 少妇人妻无码久久久久久-综合图片亚洲网友自拍| 日韩综合精品一区二区-丝袜美腿熟女人妻经典三级| 97人妻精品一区二区三区爱与-日韩精品亚洲专区在线观看| 亚洲美女喘息呻吟的网站-国产免费一区二区三区三洲| 国产传媒中文字幕在线观看-午夜福利视频在线播放观看| 中文字幕人妻少妇第一页-隔壁的女孩在线看中文字幕| 亚洲国产日韩精品四区-dy888午夜福利精品国产97| 精品一区二区三区av在线-欧美黑人巨大精品一区二区| 免费午夜福利在线观看-黄色日本黄色日本韩国黄色| 亚洲精品激情一区二区-久久成人国产欧美精品一区二区| 中文字幕偷拍亚洲九色-亚洲视频不卡一区二区天堂| 欧美日韩国产综合新一区-国产综合av一区二区三区| 成人av一区二区蜜桃-亚洲色图激情人妻欧美| 午夜福利1区2区3区-午夜洗澡免费视频网站| 日本欧美在线视频观看-国产一区二区三区无码下载快播|