HTB-20 BT-474 人乳腺導(dǎo)管癌細(xì)胞 HTB-20 BT-474 人乳腺導(dǎo)管癌細(xì)胞,ATCC 細(xì)胞|細(xì)胞系|細(xì)胞株|腫瘤細(xì)胞|細(xì)胞;細(xì)胞庫(kù)管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和*培養(yǎng)條件!
HTB-20 BT-474 人乳腺導(dǎo)管癌細(xì)胞
的詳細(xì)介紹
HTB-20 BT-474 人乳腺導(dǎo)管癌細(xì)胞
ATCC® Number: HTB-20™
Designations: BT-474
Depositors: EY Lasfargues
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent, patchy (The cells form adherent patches of epithelial-like cells The patches are compact multilayered colonies that rarely become confluent)
Organism: Homo sapiens (human)
Morphology: epithelial
HTB-20 BT-474 人乳腺導(dǎo)管癌細(xì)胞Source: Organ: mammary gland; breast
Tissue: duct
Disease: ductal carcinoma
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
HTB-20 BT-474 人乳腺導(dǎo)管癌細(xì)胞
Virus Susceptibility: mouse mammary tumor virus (RIII-MuMTV)
Tumorigenic: Yes
DNA Profile (STR): Amelogenin: X
CSF1PO: 10,11
D13S317: 11
D16S539: 9,11
D5S818: 11,13
D7S820: 9,12
THO1: 7
TPOX: 8
vWA: 15,16
Cytogenetic Analysis: The cell line is aneuploid human female (XO usually), with most chromosome counts in the hypertetraploid range. Several chromosomes (N11, N13, and N22) are absent, and others are clearly under-represented (N9, N14, and N15) with respect to the other normal chromosomes. Chromosome N7 tends towards over-representation in several karyotypes. Some of the missing normal chromosomes are represented by their involvement in the nine stable marker chromosomes: der(14)t(14;?)(q32,?), unknown, iso(13q), der(6)t(6;7)(q21;q21), der(11)t(11;?)(14;?), del(11)(p11), unknown, unknown, der(2)t(2;?)(p21;?). Several of the latter were reported by E. Lasfargues, et al. [22542]
Isoenzymes: AK-1, 1
ES-D, 1
G6PD, B
GLO-I, 1
Me-2, 0 HTB-20 BT-474 人乳腺導(dǎo)管癌細(xì)胞
PGM1, 1
PGM3, 1
Age: 60 years *
Gender: female
Ethnicity: Caucasian
Comments: The BT-474 line was isolated by E. Lasfargues and W.G. Coutinho from a solid, invasive ductal carcinoma of the breast.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC Hybri-Care Medium, Catalog No. 46-X. Hybri-Care Medium is supplied as a powder and should be reconstituted in 1 L cell-culture-grade water and supplemented with 1.5 g/L sodium bicarbonate. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Subculturing: Protocol: Remove medium, and rinse with 0.25% trypsin, 0.53 mM EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.
Subc*tion Ratio: A subc*tion ratio of 1:2 to 1:3 is recommended
Medium Renewal: 2 to 3 times per week HTB-20 BT-474 人乳腺導(dǎo)管癌細(xì)胞
Preservation: Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Related Products: recommended serum:ATCC 30-2020
Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 46-X
purified DNA:ATCC HTB-20D
References: 22542: Lasfargues EY, et al. Isolation of two human tumor epithelial cell lines from solid breast carcinomas. J. Natl. Cancer Inst. 61: 967-978, 1978. PubMed: 212572
22660: Lasfargues EY, et al. A human breast tumor cell line (BT-474) that supports mouse mammary tumor virus replication. In Vitro 15: 723-729, 1979. PubMed: 94035
32275: Littlewood-Evans AJ, et al. The osteoclast-associated protease cathepsin K is expressed in human breast carcinoma. Cancer Res. 57: 5386-5390, 1997. PubMed: 9393764
HTB-20 BT-474 人乳腺導(dǎo)管癌細(xì)胞
MLE-12 小鼠肺上皮細(xì)胞
saos-2 人成骨肉瘤細(xì)胞
MA104 非洲綠猴胚胎腎上皮細(xì)胞
ball-1 細(xì)胞
nci-h226 人肺鱗癌細(xì)胞
MCA38 小鼠結(jié)腸癌細(xì)胞
LTPA 小鼠胰腺癌細(xì)胞
NCI-H23 人非小細(xì)胞肺癌細(xì)胞
293.00 人胚腎細(xì)胞
HTB-20 BT-474 人乳腺導(dǎo)管癌細(xì)胞
293FT 人胚腎細(xì)胞-用于慢病毒包裝
293TN 人胚腎細(xì)胞--用于慢病毒包裝
293AV 人胚腎細(xì)胞-用于腺病毒包裝
293T 人胚腎細(xì)胞
208F 大鼠成纖維細(xì)胞
786-O 人腎透明細(xì)胞腺癌細(xì)胞
A172 人腦膠質(zhì)母細(xì)胞瘤細(xì)胞
A549 人肺癌細(xì)胞 細(xì)胞系購(gòu)買
AGS 人胃癌細(xì)胞
BEL7402 人肝癌細(xì)胞系
AZ-521 人胃癌細(xì)胞
B16F0 小鼠黑色素瘤細(xì)胞
Bel-7402 人肝癌細(xì)胞
Bel-7404 人肝癌細(xì)胞
BGC-823 人胃癌細(xì)胞
C6 大鼠腦膠質(zhì)瘤細(xì)胞
SW480 人結(jié)腸腺癌細(xì)胞
CaCo-2 人結(jié)腸癌細(xì)胞
Cho-K1 倉(cāng)鼠卵巢細(xì)胞亞株
COS-7 非洲綠猴SV40轉(zhuǎn)化的腎細(xì)胞
GES-1 人胃上皮細(xì)胞
HCT-8 人回盲腸癌細(xì)胞
HeLa 人宮頸癌細(xì)胞
Hepa1-6 小鼠肝癌細(xì)胞
HepG2 人肝癌細(xì)胞
hFOB1.19 人永生化成骨細(xì)胞
HGC-27 人胃癌細(xì)胞
Hs 746T 人胃癌細(xì)胞
HT-29 人結(jié)腸癌細(xì)胞
KB 人口腔表皮樣癌細(xì)胞
LoVo 人結(jié)腸癌細(xì)胞
LLC-PK1 豬腎近曲小管上皮細(xì)胞
MCF-10A 人正常乳腺上皮細(xì)胞
MCF-7 人乳腺癌細(xì)胞
MDA-MB-231 人乳腺癌細(xì)胞
MGC80-3 人胃癌細(xì)胞
MKN-45 人胃癌細(xì)胞
NCI-H460 人大細(xì)胞肺癌細(xì)胞
NIH-3T3 小鼠成纖維細(xì)胞
NUGC-3 人胃癌細(xì)胞
Raw264-7 小鼠單核細(xì)胞白血病細(xì)胞
SGC-7901 人胃癌細(xì)胞
SMMC-7721 人肝癌細(xì)胞
SNU-638 人胃癌細(xì)胞
SNU-739 人肝癌細(xì)胞
Tca8113 人舌鱗癌細(xì)胞 細(xì)胞系購(gòu)買
U251 人腦膠質(zhì)瘤細(xì)胞
U-373MG 人腦神經(jīng)膠質(zhì)瘤細(xì)胞
U-87 MG 人腦星形膠質(zhì)母細(xì)胞瘤
BT-474 人乳腺導(dǎo)管癌細(xì)胞
PANC-1 人胰腺癌細(xì)胞
HEK293 人胚腎細(xì)胞
Hep3B 人肝癌細(xì)胞
HL-7702 人正常肝細(xì)胞
Raw-Blue 小鼠單核細(xì)胞白血病細(xì)胞
WEHI-231 小鼠B淋巴細(xì)胞
OPM-2 人骨髓瘤細(xì)胞
NCI-H929 人骨髓瘤細(xì)胞
HTB-20 BT-474 人乳腺導(dǎo)管癌細(xì)胞
U266 人骨髓瘤細(xì)胞
EJ 人膀胱癌細(xì)胞
HOC 大鼠肝卵圓細(xì)胞
RWPE-1 人正常前列腺上皮細(xì)胞
CAL 27 人舌鱗癌細(xì)胞
Calu-3 人肺腺癌細(xì)胞
Eca-109 人食管癌細(xì)胞
DLD-1 人結(jié)直腸腺癌上皮細(xì)胞
5637.00 人膀胱癌細(xì)胞
SK-OV-3 人卵巢癌細(xì)胞
GBC-SD 人膽囊癌細(xì)胞
BEL7402 人肝癌細(xì)胞系
SW620 人結(jié)腸癌細(xì)胞
MRC-5 人胚肺成纖維細(xì)胞 細(xì)胞系購(gòu)買
PC9 人肺癌細(xì)胞
PC14 人肺癌細(xì)胞
BxPC 人原位胰腺腺癌細(xì)胞
PK(15) 豬腎上皮細(xì)胞
MDCK 狗腎上皮細(xì)胞
3D4/21 豬肺泡巨噬細(xì)胞
HTB-20 BT-474 人乳腺導(dǎo)管癌細(xì)胞
MHCC-97L 人肝癌細(xì)胞(低轉(zhuǎn)移)
MHCC-97H 人肝癌細(xì)胞系(高轉(zhuǎn)移)
MiaCaPa-2 人胰腺癌細(xì)胞
HN13 口腔鱗狀細(xì)胞癌細(xì)胞
Bend3 小鼠腦微血管內(nèi)皮細(xì)胞
QBC939 人膽管癌細(xì)胞
Ishikawa 人子宮內(nèi)膜癌細(xì)胞
A498 人腎癌細(xì)胞
143B
WEHI-231 小鼠B淋巴細(xì)胞
BN
P9R 小鼠骨髓基質(zhì)細(xì)胞
P9 小鼠骨髓基質(zhì)細(xì)胞
F98 大鼠腦膠質(zhì)瘤細(xì)胞
NCIH1299 人非小細(xì)胞肺癌細(xì)胞
NCIH1650-M3 人非小細(xì)胞肺癌細(xì)胞
293LVT 人胚腎細(xì)胞--用于慢病毒包裝
EC9706 人食管癌細(xì)胞
CNE 人鼻咽癌細(xì)胞
H322 人非小細(xì)胞肺癌細(xì)胞
Panc O2
S8 BEL7402 人肝癌細(xì)胞系
R5 細(xì)胞系購(gòu)買
Pig MSC 豬骨髓間質(zhì)干細(xì)胞
HCAEC 人冠狀動(dòng)脈內(nèi)皮細(xì)胞
marc-145 猴胚胎腎上皮細(xì)胞
3D4/21 豬肺泡巨噬細(xì)胞
PK(15) 豬腎上皮細(xì)胞
MADB-106 大鼠乳腺癌細(xì)胞
Sf9 昆蟲(chóng)卵巢細(xì)胞
DADY
HUVEC[HUV-EC-C] 人臍靜脈內(nèi)皮細(xì)胞
PC14 人肺癌細(xì)胞
PC9 人肺癌細(xì)胞
Eca-109 人食管癌細(xì)胞
CAL 27 人舌鱗癌細(xì)胞
HOC 肝卵圓細(xì)胞
EJ 人膀胱癌細(xì)胞
HTB-20 BT-474 人乳腺導(dǎo)管癌細(xì)胞
U266 人骨髓瘤細(xì)胞
293-mTLR5 人胚腎細(xì)胞
Raw-Blue 小鼠單核細(xì)胞白血病細(xì)胞
HL-7702 人正常肝細(xì)胞
Hep3B 人肝癌細(xì)胞
U-373MG 人腦神經(jīng)膠質(zhì)瘤細(xì)胞
Tca-8113 人舌鱗癌細(xì)胞
SNU-739 人肝癌細(xì)胞
SNU-638 人胃癌細(xì)胞
NUGC-3 人胃癌細(xì)胞
NIH-3T3 小鼠成纖維細(xì)胞
MKN-45 人胃癌細(xì)胞
LLC-PK1 豬腎近曲小管上皮細(xì)胞
Hs 746T 人胃癌細(xì)胞
Hepa1-6 小鼠肝癌細(xì)胞
HCT-8 人回盲腸癌細(xì)胞
BEL7402 人肝癌細(xì)胞系
HaCaT 人永生化表皮細(xì)胞
GES-1 人胃上皮細(xì)胞
B16-F0 小鼠黑色素瘤細(xì)胞
AZ-521 人胃癌細(xì)胞
786-O 人腎透明細(xì)胞腺癌細(xì)胞
208F 大鼠成纖維細(xì)胞
293TN 人胚腎細(xì)胞--用于慢病毒包裝
293FT 人胚腎細(xì)胞-用于慢病毒包裝
GP-293 人胚腎細(xì)胞
293-L.P. 人胚腎細(xì)胞
HTB-20 BT-474 人乳腺導(dǎo)管癌細(xì)胞