CCL-229 LoVo 人結(jié)腸癌細(xì)胞 

CCL-229 LoVo 人結(jié)腸癌細(xì)胞 的詳細(xì)介紹

ATCC® Number:  CCL-229™      
Designations:  LoVo  
Depositors:   M Romsdahl  
Biosafety Level: 1  
Shipped:  frozen  
Medium & Serum:  See Propagation  
Growth Properties: adherent 
Organism: Homo sapiens (human)  
Morphology: epithelial

 
Source: Organ: colon 
Tumor Stage: Dukes' type C, grade IV 
Disease: colorectal adenocarcinoma 
Derived from metastatic site: left supraclavicular region 
Cellular Products: carcinoembryonic antigen (CEA) 908 ng/10 exp6 cells/10 days  
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.  

Applications: transfection host (Roche FuGENE® Transfection Reagents) 
Tumorigenic: Yes  
Reverse Transcript: negative  
Oncogene: myc +; myb + ; ras +; fos +; p53 +; sis -; abl -; ros -; src -  
Antigen Expression: HLA A11, B15, B17, Cw1, Cw3; blood type B 
DNA Profile (STR): Amelogenin: XY 
CSF1PO: 10,11,13,14 
D13S317: 8, 11 
D16S539: 9, 12 
D5S818: 11, 12, 13 
D7S820: 9.3,10, 11 
TH01: 9.3 
TPOX: 8,9 
vWA: 17,18 
Cytogenetic Analysis: The stemline chromosome number is hyperdiploid with the 2S component occurring at about 2.7% and 3 marker chromosomes were common to all S metaphases. Karyotypes were generally homogeneous and stable. 
Isoenzymes:  ES-D, 1 
G6PD, B 
PGD, A 
PGM1, 2 
PGM3, 1-2 
Age:  56 years  
Gender:  male  
Comments: LoVo was initiated in 1971 from a fragment of a metastatic tumor nodule in the left supraclavicular region of a 56-year-old Caucasian male patient with a histologically proven diagnosis of adenocarcinoma of the colon. [1049] 
The cells are negative for expression of CSAp (CSAp-) and colon antigen 3. 
The line is positive for expression of c-myc, K-ras, H-ras, N-ras, Myb, sis and fos oncogenes. [22861]
Myb, and fos oncogenes. [22861] 
N-myc and sis oncogene expression were not detected. [22861] 
Tumor specific nuclear matrix proteins CC-3 and CC-4 are expressed. [23341] 
Propagation:  ATCC complete growth medium: The base medium for this cell line is ATCC-formulated F-12K Medium, Catalog No. 30-2004. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C 
Subculturing:  Subc*tion Ratio: A subc*tion ratio of 1:3 to 1:10 is recommended 
Medium Renewal: 2 to 3 times per week 
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. 
Add fresh culture medium, aspirate and dispense into new culture flasks. 
Preservation:  culture medium 95%; DMSO, 5% 
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2004
recommended serum:ATCC 30-2020 
References: 1047: Drewinko B, et al. Further biologic characteristics of a human carcinoembryonic antigen-producing colon carcinoma cell line. J. Natl. Cancer Inst. 61: 75-83, 1978. PubMed: 276641
1048: Drewinko B, Yand LY. Restriction of CEA synthesis to the stationary phase of growth of cultured human colon carcinoma cells. Exp. Cell Res. 101: 414-416, 1976. PubMed: 964319
1049: Drewinko B, et al. Establishment of a human carcinoembryonic antigen-producing colon adenocarcinoma cell line. Cancer Res. 36: 467-475, 1976. PubMed: 1260746  
22861: Trainer DL, et a l. Biological characterization and oncogene expression in human colorectal carcinoma cell lines. Int. J. Cancer 41: 287-296, 1988. PubMed: 3338874
23341: Keesee SK, et al. Nuclear matrix proteins in human colon cancer. Proc. Natl. Acad. Sci. USA 91: 1913-1916, 1994. PubMed: 8127905
26057: Drewinko B, et al. Response of exponentially growing, stationary-phase, and synchronized cultured human colon carcinoma cells to treatment with nitrosourea derivatives. Cancer Res. 39: 2630-2636, 1979. PubMed: 445465
32913: Miranda L, et al. Isolation of the human PC6 gene encoding the putative host protease for HIV-1 gp160 processing in CD4+ T lymphocytes. Proc. Natl. Acad. Sci. USA 93: 7695-7700, 1996. PubMed: 8755538 

MLE-12 小鼠肺上皮細(xì)胞
saos-2 人成骨肉瘤細(xì)胞
MA104 非洲綠猴胚胎腎上皮細(xì)胞
ball-1 細(xì)胞
nci-h226 人肺鱗癌細(xì)胞
MCA38 小鼠結(jié)腸癌細(xì)胞
LTPA 小鼠胰腺癌細(xì)胞 
NCI-H23 人非小細(xì)胞肺癌細(xì)胞
293.00     人胚腎細(xì)胞

293FT    人胚腎細(xì)胞-用于慢病毒包裝
293TN    人胚腎細(xì)胞--用于慢病毒包裝
293AV    人胚腎細(xì)胞-用于腺病毒包裝
293T    人胚腎細(xì)胞
208F    大鼠成纖維細(xì)胞
786-O    人腎透明細(xì)胞腺癌細(xì)胞
A172    人腦膠質(zhì)母細(xì)胞瘤細(xì)胞
A549    人肺癌細(xì)胞 細(xì)胞系購買
AGS    人胃癌細(xì)胞
BEL7402 人肝癌細(xì)胞系
AZ-521    人胃癌細(xì)胞
B16F0    小鼠黑色素瘤細(xì)胞
Bel-7402    人肝癌細(xì)胞
Bel-7404    人肝癌細(xì)胞
BGC-823    人胃癌細(xì)胞
C6    大鼠腦膠質(zhì)瘤細(xì)胞
SW480    人結(jié)腸腺癌細(xì)胞
CaCo-2    人結(jié)腸癌細(xì)胞
Cho-K1    倉鼠卵巢細(xì)胞亞株
COS-7    非洲綠猴SV40轉(zhuǎn)化的腎細(xì)胞
GES-1    人胃上皮細(xì)胞
HCT-8    人回盲腸癌細(xì)胞
HeLa    人宮頸癌細(xì)胞
Hepa1-6    小鼠肝癌細(xì)胞
HepG2    人肝癌細(xì)胞
hFOB1.19    人永生化成骨細(xì)胞
HGC-27    人胃癌細(xì)胞
Hs 746T    人胃癌細(xì)胞
HT-29    人結(jié)腸癌細(xì)胞
KB    人口腔表皮樣癌細(xì)胞
LoVo    人結(jié)腸癌細(xì)胞
LLC-PK1    豬腎近曲小管上皮細(xì)胞
MCF-10A    人正常乳腺上皮細(xì)胞
MCF-7    人乳腺癌細(xì)胞
MDA-MB-231    人乳腺癌細(xì)胞
MGC80-3    人胃癌細(xì)胞
MKN-45    人胃癌細(xì)胞
NCI-H460    人大細(xì)胞肺癌細(xì)胞
NIH-3T3    小鼠成纖維細(xì)胞
NUGC-3    人胃癌細(xì)胞
Raw264-7    小鼠單核細(xì)胞白血病細(xì)胞
SGC-7901    人胃癌細(xì)胞
SMMC-7721    人肝癌細(xì)胞
SNU-638    人胃癌細(xì)胞
SNU-739    人肝癌細(xì)胞
Tca8113    人舌鱗癌細(xì)胞    細(xì)胞系購買
U251    人腦膠質(zhì)瘤細(xì)胞
U-373MG    人腦神經(jīng)膠質(zhì)瘤細(xì)胞
U-87 MG    人腦星形膠質(zhì)母細(xì)胞瘤
BT-474    人乳腺導(dǎo)管癌細(xì)胞
PANC-1    人胰腺癌細(xì)胞
HEK293    人胚腎細(xì)胞
Hep3B    人肝癌細(xì)胞
HL-7702    人正常肝細(xì)胞
Raw-Blue    小鼠單核細(xì)胞白血病細(xì)胞
WEHI-231    小鼠B淋巴細(xì)胞
OPM-2    人骨髓瘤細(xì)胞
NCI-H929    人骨髓瘤細(xì)胞

U266    人骨髓瘤細(xì)胞
EJ    人膀胱癌細(xì)胞
HOC    大鼠肝卵圓細(xì)胞
RWPE-1    人正常前列腺上皮細(xì)胞
CAL 27    人舌鱗癌細(xì)胞
Calu-3    人肺腺癌細(xì)胞
Eca-109    人食管癌細(xì)胞
DLD-1    人結(jié)直腸腺癌上皮細(xì)胞
5637.00     人膀胱癌細(xì)胞
SK-OV-3    人卵巢癌細(xì)胞
GBC-SD    人膽囊癌細(xì)胞
BEL7402 人肝癌細(xì)胞系
SW620    人結(jié)腸癌細(xì)胞
MRC-5    人胚肺成纖維細(xì)胞     細(xì)胞系購買
PC9    人肺癌細(xì)胞
PC14    人肺癌細(xì)胞
BxPC    人原位胰腺腺癌細(xì)胞
PK（15）    豬腎上皮細(xì)胞
MDCK    狗腎上皮細(xì)胞
3D4/21    豬肺泡巨噬細(xì)胞
    
    
    
MHCC-97L    人肝癌細(xì)胞（低轉(zhuǎn)移）
MHCC-97H    人肝癌細(xì)胞系（高轉(zhuǎn)移）
MiaCaPa-2    人胰腺癌細(xì)胞
HN13    口腔鱗狀細(xì)胞癌細(xì)胞
Bend3    小鼠腦微血管內(nèi)皮細(xì)胞
QBC939    人膽管癌細(xì)胞
Ishikawa    人子宮內(nèi)膜癌細(xì)胞
A498    人腎癌細(xì)胞
143B    
WEHI-231    小鼠B淋巴細(xì)胞
BN    
P9R    小鼠骨髓基質(zhì)細(xì)胞
P9    小鼠骨髓基質(zhì)細(xì)胞
F98    大鼠腦膠質(zhì)瘤細(xì)胞
NCIH1299    人非小細(xì)胞肺癌細(xì)胞
NCIH1650-M3    人非小細(xì)胞肺癌細(xì)胞
293LVT    人胚腎細(xì)胞--用于慢病毒包裝
EC9706    人食管癌細(xì)胞
CNE    人鼻咽癌細(xì)胞
H322    人非小細(xì)胞肺癌細(xì)胞
Panc O2    
S8    BEL7402 人肝癌細(xì)胞系
R5    細(xì)胞系購買
Pig MSC    豬骨髓間質(zhì)干細(xì)胞
HCAEC    人冠狀動脈內(nèi)皮細(xì)胞
marc-145    猴胚胎腎上皮細(xì)胞
3D4/21    豬肺泡巨噬細(xì)胞
PK（15）    豬腎上皮細(xì)胞
MADB-106    大鼠乳腺癌細(xì)胞
Sf9    昆蟲卵巢細(xì)胞
DADY    
HUVEC[HUV-EC-C]    人臍靜脈內(nèi)皮細(xì)胞
PC14    人肺癌細(xì)胞
PC9    人肺癌細(xì)胞
Eca-109    人食管癌細(xì)胞
CAL 27    人舌鱗癌細(xì)胞
HOC    肝卵圓細(xì)胞
EJ    人膀胱癌細(xì)胞

U266    人骨髓瘤細(xì)胞
293-mTLR5    人胚腎細(xì)胞
Raw-Blue    小鼠單核細(xì)胞白血病細(xì)胞
HL-7702    人正常肝細(xì)胞
Hep3B    人肝癌細(xì)胞
U-373MG    人腦神經(jīng)膠質(zhì)瘤細(xì)胞
Tca-8113    人舌鱗癌細(xì)胞
SNU-739    人肝癌細(xì)胞
SNU-638    人胃癌細(xì)胞
NUGC-3    人胃癌細(xì)胞
NIH-3T3    小鼠成纖維細(xì)胞
MKN-45    人胃癌細(xì)胞
LLC-PK1    豬腎近曲小管上皮細(xì)胞
Hs 746T    人胃癌細(xì)胞
Hepa1-6    小鼠肝癌細(xì)胞
HCT-8    人回盲腸癌細(xì)胞
BEL7402 人肝癌細(xì)胞系
HaCaT    人永生化表皮細(xì)胞
GES-1    人胃上皮細(xì)胞
B16-F0    小鼠黑色素瘤細(xì)胞
AZ-521    人胃癌細(xì)胞
786-O    人腎透明細(xì)胞腺癌細(xì)胞
208F    大鼠成纖維細(xì)胞
293TN    人胚腎細(xì)胞--用于慢病毒包裝
293FT    人胚腎細(xì)胞-用于慢病毒包裝
GP-293    人胚腎細(xì)胞
293-L.P.    人胚腎細(xì)胞