CRL-1735 Lec1 倉(cāng)鼠卵巢細(xì)胞，ATCC 細(xì)胞|細(xì)胞系|細(xì)胞株|腫瘤細(xì)胞|細(xì)胞|貼壁細(xì)胞|懸浮細(xì)胞|,細(xì)胞庫(kù)管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和*培養(yǎng)條件
CRL-1735 Lec1 倉(cāng)鼠卵巢細(xì)胞，ATCC 細(xì)胞|細(xì)胞系|細(xì)胞株|腫瘤細(xì)胞|細(xì)胞|貼壁細(xì)胞|懸浮細(xì)胞|,細(xì)胞庫(kù)管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和*培養(yǎng)條件
CRL-1735 Lec1 倉(cāng)鼠卵巢細(xì)胞 的詳細(xì)介紹
CRL-1735 Lec1 倉(cāng)鼠卵巢細(xì)胞
ATCC? Number: CRL-1735?
Designations: Lec1 [originally named Pro-5WgaRI3C]
Depositors: P Stanley
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: loosely adherent
Organism: Cricetulus griseus （hamster, Chinese）
Morphology: epithelial
Source: Organ: ovary
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Applications: transfection host （Roche FuGENE? Transfection Reagents）
Gender: female
Comments: This line is a mutant clone derived from the parental CHO clone Pro-5 （a proline auxotroph, see ATCC CRL-1781） by selection for resistance to wheat germ agglutinin. The cells lack GlcNAc glycosyl transferase so that N-linked carbohydrates are blocked at the Man5-GlcNAC2-Asn intermediate. The line may be useful for studying the effects of altered N-linked glycosylation on the function and compartmentalization of endogenous glycoproteins or glycoproteins introduced by viral infection or transfection of foreign DNA.
BR>The population contains Pro+ revertants at low frequency （approximay 1 in 100,000 cells）, and should be suitable for complementation studies which require the Pro- marker.
The cells are proline auxotrophs and must be grown in a medium that contains proline （40 mg/L）.
The population contains Pro+ revertants at low frequency （approximay 1 in 100000 cells）, and should be suitable for complementation studies which require the Pro- marker.
Propagation: ATCC complete growth medium: Alpha minimum essential medium with ribonucleosides and deoxyribonucleosides, 90%; fetal bovine serum, 10%
Temperature: 37.0°C
Subculturing: Protocol: Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature （or at 37C） until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.
Subc*tion Ratio: A subc*tion ratio of 1:2 to 1:4 is recommended
Medium Renewal: 2 to 3 times per week
Preservation: Freeze medium: Complete growth medium 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Related Products: recommended serum:ATCC 30-2020
References: 1201: Stanley P, et al. Selection and characterization of eight phenotypically distinct lines of lectin-resistant Chinese hamster ovary cells. Cell 6: 121-128, 1975. PubMed: 1182798
23255: Stanley P, et al. Chinese hamster ovary cells selected for resistance to the cytotoxicity of phytohemagglutinin are deficient in a UDP-N-acetylglucosamine-- glycoprotein N-acetylglucosaminyltransferase activity. Proc. Natl. Acad. Sci. USA 72: 3323-3327, 1975. PubMed: 1059116
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