NRK-49F 大鼠正常腎成纖維細胞， ATCC 細胞|細胞系|細胞株|腫瘤細胞|細胞|貼壁細胞|懸浮細胞|,細胞庫管理規(guī)范,提供的細胞株背景清楚,提供參考文獻和*培養(yǎng)條件
NRK-49F 大鼠正常腎成纖維細胞， ATCC 細胞|細胞系|細胞株|腫瘤細胞|細胞|貼壁細胞|懸浮細胞|,細胞庫管理規(guī)范,提供的細胞株背景清楚,提供參考文獻和*培養(yǎng)條件
NRK-49F 大鼠正常腎成纖維細胞 的詳細介紹
NRK-49F 大鼠正常腎成纖維細胞
| ATCC? Number: | CRL-1570? | Price: | $329.00 |
| Designations: | NRK-49F | | Depositors: | JE DeLarco | | Biosafety Level: | 1 | | Shipped: | frozen | | Medium & Serum: | See Propagation | | Growth Properties: | adherent | | Organism: | Rattus norvegicus （rat） | | Morphology: | fibroblast
| | Source: | Organ: kidney
Disease: normal | | Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. |
| | Applications: | transfection host （Roche FuGENE? Transfection Reagents） | | Receptors: | epidermal growth factor （EGF）; multiplication stimulating activity （MSA） | | Comments: | NRK-49F cells are contact inhibited and very sensitive to transformation by chemicals and viruses.
Cultures should be subcultured before they become confluent.
If allowed to become confluent, the cells will transform. | | Propagation: | ATCC complete growth medium: Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose, 95%; bovine calf serum, 5%
Temperature: 37.0°C
Atmosphere: air, 95%; carbon dioxide （CO2）, 5% | | Subculturing: | Subc*tion Ratio: A subc*tion ratio of 1:3 to 1:4 is recommended
Medium Renewal: Twice per week
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature （or at 37C） until the cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks. | | Preservation: | culture medium 95%; DMSO, 5% | | Related Products: | Recommended medium （without the additional supplements or serum described under ATCC Medium）:ATCC 30-2002 | | References: | 22713: de Larco JE, Todaro GJ. Epithelioid and fibroblastic rat kidney cell clones: epidermal growth factor （EGF） receptors and the effect of mouse sarcoma virus transformation. J. Cell. Physiol. 94: 335-342, 1978. PubMed: 304450
23197: Huu D, et al. Persistent infection of a rat kidney cell line with Rauscher murine leukemia virus. J. Bacteriol. 92: 1133-1140, 1966. PubMed: 5926742
32442: Sachsenmeier KF, et al. Transforming growth factor-beta1 inhibits nucleosomal fragmentation in human keratinocytes following loss of adhesion. J. Biol. Chem. 271: 5-8, 1996. PubMed: 8550610
32705: Schultz-Cherry S, Hinshaw VS. Influenza virus neuraminidase activates latent transforming growth factor beta. J. Virol. 70: 8624-8629, 1996. PubMed: 8970987
33046: Panneerselvam K, Freeze HH. Mannose enters mammalian cells using a specific transporter that is insensitive to glucose. J. Biol. Chem. 271: 9417-9421, 1996. PubMed: 8621609
33159: Centrella M, et al. Rapid flux in transforming growth factor-beta receptors on bone cells. J. Biol. Chem. 271: 18616-18622, 1996. PubMed: 8702513 |
|
14
