欧美……一区二区三区,欧美日韩亚洲另类视频,亚洲国产欧美日韩中字,日本一区二区三区dvd视频在线

上海復(fù)祥生物科技有限公司

當(dāng)前位置:> 供求商機(jī)> 低價(jià)供應(yīng) CRL-2638 CT26.WT 小鼠結(jié)腸癌細(xì)胞

[供應(yīng)]低價(jià)供應(yīng) CRL-2638 CT26.WT 小鼠結(jié)腸癌細(xì)胞

貨物所在地:上海上海市

更新時(shí)間:2024-10-13 21:00:05

有效期:2024年10月13日 -- 2025年4月13日

已獲點(diǎn)擊:151

聯(lián)系我時(shí),請(qǐng)告知來(lái)自 化工儀器網(wǎng)

CRL-2638 CT26.WT 小鼠結(jié)腸癌細(xì)胞,原代細(xì)胞|細(xì)胞系|細(xì)胞株|菌種;細(xì)胞庫(kù)管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和*培養(yǎng)條件!

CRL-2638 CT26.WT 小鼠結(jié)腸癌細(xì)胞 的詳細(xì)介紹

CRL-2638 CT26.WT 小鼠結(jié)腸癌細(xì)胞

ATCC? Number: CRL-2638?    Price:
Designations: CT26.WT
Depositors:  N Restifo
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties:adherent
Organism:Mus musculus (mouse)
Morphology:fibroblast

Source:Strain: BALB/c
Organ: colon
Disease: carcinoma
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Tumorigenic:Yes
Antigen Expression:H-2d [53315]
Comments:CT26 is an N-nitroso-N-methylurethane-(NNMU) induced, undifferentiated colon carcinoma cell line. It was cloned to generate the cell line designated CT26.WT (ATCC CRL-2638).
CT26.WT was stably transduced with the retroviral vector LXSN that contains the lacZ gene encoding the model tumor associated antigen (TAA), beta-galactosidase (beta-gal) to obtain the lethal subclone CT26.CL25 (ATCC CRL-2639). [53315]
The growth rate and lethality of CT26.CL25 and CT26.WT is virtually identical despite the expression by CT26.CL25 of the model TAA, beta-galactosidase, in normal mice. [53315]
The cell line can be used with CT26.CL25 (ATCC CRL-2639) as a model for testing immunotherapy protocols and in studies on the host immune response.
A culture submitted to the ATCC in July of 2001 was found to be contaminated with mycoplasma. Progeny were cured by a 21-day treatment with BM Cycline.
The cells were assayed for mycoplasma, by the Hoechst stain, PCR and the standard culture test, after a six-week period following treatment. All tests were negative.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10.
Atmosphere: air, 95; carbon dioxide (CO2), 5
Temperature: 37.0°C
Subculturing: Protocol:
  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 0.25 (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
  3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
  5. Add appropriate aliquots of the cell suspension to new culture vessels.
  6. Incubate cultures at 37°C.

Subc*tion Ratio: A subc*tion ratio of 1:4 to 1:10 is recommended
Medium Renewal: Every 2 to 3 days
Preservation: Freeze medium: Complete growth medium supplemented with 5 (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001
recommended serum:ATCC 30-2020
derivative:ATCC CRL-2639
References:53315: Wang M, et al. Active immunotherapy of cancer with a nonreplicating recombinant fowlpox virus encoding a model tumor-associated antigen. J. Immunol. 154: 4685-4692, 1995. PubMed: 7722321


會(huì)員登錄

X

請(qǐng)輸入賬號(hào)

請(qǐng)輸入密碼

=

請(qǐng)輸驗(yàn)證碼

收藏該商鋪

X
該信息已收藏!
標(biāo)簽:
保存成功

(空格分隔,最多3個(gè),單個(gè)標(biāo)簽最多10個(gè)字符)

常用:

提示

X
您的留言已提交成功!我們將在第一時(shí)間回復(fù)您~

以上信息由企業(yè)自行提供,信息內(nèi)容的真實(shí)性、準(zhǔn)確性和合法性由相關(guān)企業(yè)負(fù)責(zé),化工儀器網(wǎng)對(duì)此不承擔(dān)任何保證責(zé)任。

溫馨提示:為規(guī)避購(gòu)買風(fēng)險(xiǎn),建議您在購(gòu)買產(chǎn)品前務(wù)必確認(rèn)供應(yīng)商資質(zhì)及產(chǎn)品質(zhì)量。

撥打電話
在線留言
亚洲愉拍自拍欧美精品app-亚洲一区不卡在线视频| 91精品国产无线乱码在线-999精品视频免费看| 国产欧美成人精品第一区-日本黄色精品一区二区| 久久99热这里都是精品啊-国产成人亚洲精品无码aV| 91精品国产影片一区二区三区-欧美精品久久久精品一区二区| 国产午夜精品理论片A级漫画-久久精品国产99亚洲精品| 黑丝av少妇精品久久久久久久-中文字幕久久久人妻无码| 99在线观看精品视频免费-国产极品一区二区三区四区| 中文字幕偷拍亚洲九色-亚洲视频不卡一区二区天堂| 久艹在线观看视频免费-人妻偷人精品一区二区三区| 俄罗斯胖老太太黄色特级片-国产精品黑丝美腿美臀| 婷婷综合在线视频观看-亚洲一区二区三区香蕉| 99精品只有久久精品免费-蜜臀一区二区三区精品久久久| 青青操视频在线观看国产-欧美成人乱码在线观看| 蜜臀一区二区三区精品在线-99久久久精品免费看国产| 久久99热这里都是精品啊-国产成人亚洲精品无码aV| 看肥婆女人黄色儿逼视频-秋霞电影一区二区三区四区| 黄片一区二区三区在线看-偷拍一区二区在线观看| 麻豆久久国产精品亚洲-日本理论中文字幕在线视频| 亚洲永久免费在线观看-亚洲欧美导航一区二区导航| 国产精品一区二区欧美视频-国产一区二区三区天码| 亚洲欧洲成视频免费观看-国产福利一区二区在线观看| 黄色美女网站大全中文字幕-欧美韩国日本一区二区| 亚洲综合av一区二区三区-高潮又爽又黄无遮挡激情视频| 国产av剧情护士麻豆-三级国产精品欧美在线观看| av中文字幕男人天堂-懂色av一区二区三区在线观看| 激情字幕久久久字幕中文-一区二区三区免费黄片| 国产精品成人欧美激情-黄色床上完整版高清无遮挡| 7m视频7m精品视频网站-亚洲综合香蕉视频在线| 美女把腿张开给帅哥桶-无码无套少妇18p在线直播| 国产精品电影在线一区-亚洲国产大片一区二区官网| 亚洲欧美日韩二区三区-国产在线欧美一区日韩二区| 国产在线观看高清精品-四季av一区二区三区中文字幕| 国产午夜精品理论片A级漫画-久久精品国产99亚洲精品| 可以免费看污污视频的网站-日韩欧美不卡视频在线观看| 91精品国产无线乱码在线-999精品视频免费看| 激情字幕久久久字幕中文-一区二区三区免费黄片| 亚洲黄片三级三级三级-国产成人一区二区在线视频| 97一区二区三区在线-欧美护士性极品hd4k| 天天干天天天天天天天-亚洲综合av在线三区| 国产aa视频一区二区三区-国产精品久久久久久久毛片动漫|