CCL-9.1 NCTC 1469 小鼠正常肝細胞，原代細胞|細胞系|細胞株|菌種；細胞庫管理規(guī)范，提供的細胞株背景清楚，提供參考文獻和*培養(yǎng)條件！
CCL-9.1 NCTC 1469 小鼠正常肝細胞 的詳細介紹
CCL-9.1 NCTC 1469 小鼠正常肝細胞
| ATCC? Number: | CCL-9.1? | Price: | $329.00 |
| Designations: | NCTC clone 1469 [derivative of NCTC 721] | | Depositors: | VJ Evans | | Biosafety Level: | 1 | | Shipped: | frozen | | Medium & Serum: | See Propagation | | Growth Properties: | adherent | | Organism: | Mus musculus （mouse） | | Morphology: | epithelial
| | Source: | Organ: liver
Strain: C3H/An
Disease: normal | | Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. |
| | Isolation: | Isolation date: April, 1952 | | Virus Susceptibility: | Vesicular stomatitis virus
Human poliovirus 1 | | Antigen Expression: | H-2k | | Cytogenetic Analysis: | modal number = 41; range = 38 to 86.
Two marker chromosomes present: One metacentric with one pair of arms having achromatic gaps near centromere; and an acrocentric with salites. | | Age: | newborn | | Gender: | male | | Comments: | Tested and found negative for ectromelia virus （mousepox）. | | Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: horse serum to a final concentration of 10%.
Atmosphere: air, 95%; carbon dioxide （CO2）, 5%
Temperature: 37.0°C | | Subculturing: | Protocol: Subcultures are prepared by scraping. Remove old medium, add fresh medium and dislodge the cells from the floor of the flask. Aspirate cells and dispense the suspension into new flasks.
A standard trypsinizaton may be used if desired.
The culture medium in heavy cultures may be turbid in appearance and and give the gross impression of bacterial contamination.
It is characteristic of this line to shed viable cells into the medium, thus rendering the medium turbid. The shed cells are viable and may be used to initiate new cultures.
Subc*tion Ratio: A subc*tion ratio of 1:2 to 1:4 is recommended
Medium Renewal: 3 times per week | | Preservation: | Freeze medium: Complete growth medium supplemented with 5% （v/v） DMSO
Storage temperature: liquid nitrogen vapor phase | | Related Products: | Recommended medium （without the additional supplements or serum described under ATCC Medium）:ATCC 30-2002
recommended serum:ATCC 30-2040 | | References: | 22167: Hobbs GL, et al. Establishment of a clone of mouse liver cells from a single isolated cell. J. Natl. Cancer Inst. 18: 701-707, 1957. PubMed: 13502690
26070: Evans VJ, et al. The growth in vitro of massive cultures of liver cells. J. Natl. Cancer Inst. 12: 1245-1265, 1952. PubMed: 14939026
26074: . . J. Natl. Cancer Inst. 23: 823, 1959.
26076: . . J. Natl. Cancer Inst. 27: 29, 1961.
26078: Evans VJ, et al. Studies on culture lines derived from mouse liver parenchymatous cells grown in long-term tissue culture. Cancer Res. 12: 261-266, 1958. PubMed: 13523589
26079: . . Fed. Proc. 17: 967, 1958.
26081: Westfall BB. Characterization of cells in tissue culture. Natl. Cancer Inst. Monogr. 7: 147-158, 1962. PubMed: 14006338 |
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