欧美……一区二区三区,欧美日韩亚洲另类视频,亚洲国产欧美日韩中字,日本一区二区三区dvd视频在线

上海復(fù)祥生物科技有限公司

當(dāng)前位置:> 供求商機> 低價供應(yīng) CRL-2749 OP9 小鼠骨髓基質(zhì)細(xì)胞

[供應(yīng)]低價供應(yīng) CRL-2749 OP9 小鼠骨髓基質(zhì)細(xì)胞

貨物所在地:上海上海市

更新時間:2024-10-13 21:00:05

有效期:2024年10月13日 -- 2025年4月13日

已獲點擊:281

聯(lián)系我時,請告知來自 化工儀器網(wǎng)

CRL-2749 OP9 小鼠骨髓基質(zhì)細(xì)胞,ATCC 細(xì)胞|細(xì)胞系|細(xì)胞株|腫瘤細(xì)胞|細(xì)胞,細(xì)胞庫管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻和*培養(yǎng)條件,

CRL-2749 OP9 小鼠骨髓基質(zhì)細(xì)胞 的詳細(xì)介紹

CRL-2749 OP9 小鼠骨髓基質(zhì)細(xì)胞

ATCC? Number: CRL-2749?    Price:
Designations: OP9
Depositors:  T Nakano
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties:adherent
Organism:Mus musculus (mouse)
Morphology:fibroblast

Source:Organ: bone marrow
Strain: (C57BL/6 x C3H)F2 -op/op
Tissue: stroma
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Applications:supports hematopoietic differention
Age: newborn newborn
Comments:The OP9 cell line was established from newborn op/op mouse calvaria. The cells do not produce functional macrophage colony-stimulating factor (M-CSF) due to an osteopetrotic mutation in the gene encoding M-CSF. The presence of M-CSF had inhibitory effects on the differentiation of embryonic stem (ES) cells to blood cells other than macrophages. OP9 cells can be used to coculture mouse embryonic stem cells (ES cells) to induce the differentiation of embryonic stem (ES) cells into blood cells of erythroid, myeloid, and B cell lineages. Coc*tion with OP9 does not require exogenous growth factors or complex embryoid structures. This system will facilitate the study of molecular mechanisms involved in development and differentiation of hematopoietic cells.
Propagation: ATCC complete growth medium: The base medium for this cell line is Alpha Minimum Essential medium without ribonucleosides and deoxyribonucleosides but with 2 mM L-glutamine and 1.5 g/L sodium bicarbonate . To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20%
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Subculturing: Protocol: Note: Cell density is important. If the subculture ratio is too low, the culture will not reach confluence. However, do not overgrow. Very large cells tend to appear after overgrowth and these cells are a warning sign that the OP9 cells will not support the maintenance of hematopoietic cells. Subculture just before confluence.
  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
  3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
  5. Transfer cell suspension to a centrifuge tube and spin at approximay 125 X g for 5 to 10 minutes. Discard supernatant.
  6. Resuspend the cell pellet in fresh growth medium. Add appropriate aliquots of the cell suspension to new culture vessels.
  7. Incubate cultures at 37°C.
    Interval: Maintain cultures at a cell concentration between 4 X 10(3) and 1 X 10(4) cells/cm2.
    Subc*tion Ratio: A subc*tion ratio of 1:4 to 1:5 is recommended
    Medium Renewal: Every 2 to 3 days
Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Doubling Time: 26 hrs
Related Products:recommended serum:ATCC 30-2020
References:61302: Nakano T, et al. Generation of lymphohematopoietic cells from embryonic stem cells in culture. Science 265: 1098-1101, 1994. PubMed: 8066449
64482: Nakano T, et al. In vitro development of primitive and definitive erythrocytes from different precursors. Science 272: 722-724, 1996. PubMed: 8614833
64484: Nakano T. Lymphohematopoietic development from embryonic stem cells in vitro. Semin. Immunol. 7: 197-203, 1995. PubMed: 7579206
64485: Motoyama N, et al. bcl-x prevents apoptotic cell death of both primitive and definitive erythrocytes at the end of maturation. J. Exp. Med. 189: 1691-1698, 1999. PubMed: 10359572
64486: Nakano T. In vitro development of hematopoietic system from mouse embryonic stem cells: a new approach for embryonic hematopoiesis. Int. J. Hematol. 65: 1-8, 1996. PubMed: 8990620
64487: Nakano T, et al. Development of erythroid cells from mouse embryonic stem cells in culture: potential use for erythroid transcription factor study. Leukemia 3: 496-500, 1997. PubMed: 9209437
64488: Suwabe N, et al. GATA-1 regulates growth and differentiation of definitive erythroid lineage cells during in vitro ES cell differentiation. Blood 92: 4108-4118, 1998. PubMed: 9834216
64489: Suzuki A, Nakano T. Development of hematopoietic cells from embryonic stem cells. Int. J. Hematol. 73: 1-5, 2001. PubMed: 11372743
64490: Eto K, et al. Megakaryocytes derived from embryonic stem cells implicate CalDAG-GEFI in integrin signaling. Proc. Natl. Acad. Sci. USA 99: 12819-12824, 2002. PubMed: 12239348


會員登錄

X

請輸入賬號

請輸入密碼

=

請輸驗證碼

收藏該商鋪

X
該信息已收藏!
標(biāo)簽:
保存成功

(空格分隔,最多3個,單個標(biāo)簽最多10個字符)

常用:

提示

X
您的留言已提交成功!我們將在第一時間回復(fù)您~

以上信息由企業(yè)自行提供,信息內(nèi)容的真實性、準(zhǔn)確性和合法性由相關(guān)企業(yè)負(fù)責(zé),化工儀器網(wǎng)對此不承擔(dān)任何保證責(zé)任。

溫馨提示:為規(guī)避購買風(fēng)險,建議您在購買產(chǎn)品前務(wù)必確認(rèn)供應(yīng)商資質(zhì)及產(chǎn)品質(zhì)量。

撥打電話
在線留言
在线观看中午中文乱码-2021国产一级在线观看| 韩漫一区二区在线观看-精品国产免费未成女一区二区三区| 亚洲国产日韩精品四区-dy888午夜福利精品国产97| 91精品国产无线乱码在线-999精品视频免费看| 日韩精品一区二区三区十八-日韩人妻少妇一区二区三区| 国产成人精品免费视频大全办公室-亚洲欧美日本综合在线| 日韩综合精品一区二区-丝袜美腿熟女人妻经典三级| 中文字幕日本在线资源-国产+成+人+亚洲欧洲自线| 日韩高清在线观看一区二区-美产av在线免费观看| 欧美mv日韩mv视频-熟妇人妻ⅴa精品中文| 欧美aa一级视频播放-久一一区二区三区大香蕉| 国产精品二区高清在线-91精品91久久久久久| 日本韩国亚洲欧美三级-日本东京不卡网一区二区三区| 亚洲午夜久久久精品影院-性感美女在线观看网站国产| 精品亚洲卡一卡2卡三卡乱码-一区三区二插女人高潮在线观看| 亚洲欧美日韩二区三区-国产在线欧美一区日韩二区| hd在线观看一区二区-免费一区二区三区毛片在线| 亚洲av日韩五月天久热精品-国产日韩欧美一区二区三区群战| 亚洲一区二区三在线观看-国产精品亚洲а∨天堂123| 中文字幕亚洲综合久久最新-久久精品视频免费久久久| 中文字幕日韩不卡久久-五月天中文字幕啊av| 欧美成人精品巨臀大屁股-亚洲综合欧美日韩一区| 一区二区国产高清在线-日本高清无卡一区二区三区| 久久影视av一区二区-人妻激情乱偷一区二区三区| 亚洲欧美另类综合偷拍-婷婷社区综合在线观看| 天天干天天天天天天天-亚洲综合av在线三区| 国产精品成人欧美激情-黄色床上完整版高清无遮挡| 在线观看中午中文乱码-2021国产一级在线观看| 日本女优一卡二卡在线观看-欧美大胆a级视频秒播| 亚洲女人黄色录像一区-日韩av电影在线免费看| 中文字幕亚洲综合久久最新-久久精品视频免费久久久| 青青操大香蕉在线播放-国产亚洲欧美精品在线观看| 日韩精品中文在线观看一区-亚洲bt欧美bt精品| 亚洲欧美一区二区中文-台湾中文综合网妹子网| 看肥婆女人黄色儿逼视频-秋霞电影一区二区三区四区| 精彩亚洲一区二区三区-中文字幕中文字幕在线色站| 白嫩美女娇喘呻吟高潮-久久一区二区三区日产精品| 韩漫一区二区在线观看-精品国产免费未成女一区二区三区| 国产精品二区高清在线-91精品91久久久久久| 免费午夜福利视频在线观看-亚洲成人日韩欧美伊人一区| 日韩精品中文在线观看一区-亚洲bt欧美bt精品|