欧美……一区二区三区,欧美日韩亚洲另类视频,亚洲国产欧美日韩中字,日本一区二区三区dvd视频在线

上海復(fù)祥生物科技有限公司

當(dāng)前位置:> 供求商機(jī)> CRL-2190-CRL-2190 HK-2 人腎近曲小管上皮細(xì)胞

[供應(yīng)]CRL-2190-CRL-2190 HK-2 人腎近曲小管上皮細(xì)胞

貨物所在地:上海上海市

產(chǎn)地:美國

更新時(shí)間:2024-04-01 16:25:21

有效期:2024年4月1日 -- 2025年4月1日

已獲點(diǎn)擊:83

聯(lián)系我時(shí),請(qǐng)告知來自 化工儀器網(wǎng)

CRL-2190 HK-2 人腎近曲小管上皮細(xì)胞,原代細(xì)胞|細(xì)胞系|細(xì)胞株|菌種;細(xì)胞庫管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和*培養(yǎng)條件!

CRL-2190 HK-2 人腎近曲小管上皮細(xì)胞

ATCC® Number:CRL-2190™    Price:$272.00
Designations:HK-2
Depositors: RA Zager
Biosafety Level:2 [Cells Contain Papilloma viral DNA sequences ]
Shipped:frozen
Medium & Serum:See Propagation
Growth Properties:adherent
Organism:Homo sapiens (human)
Morphology:epithelial
 
Source:Organ: kidney, cortex 
Tissue: proximal tubule 
Cell Type: human papillomavirus 16 (HPV-16) transformed
Cellular Products:alkaline phosphatase; gamma glutamyltranspeptidase; leucine aminopeptidase; acid phosphatase; cytokeratin; alpha 3, beta 1 integrin; fibronectin
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
 
Receptors:epidermal growth factor (EGF), expressed
DNA Profile (STR):Amelogenin: X,Y 
CSF1PO: 13 
D13S317: 9 
D16S539: 11,12 
D5S818: 12 
D7S820: 10,11 
THO1: 9 
TPOX: 8,9 
vWA: 17,18
Age:*****
Gender:male
Comments:HK-2 (human kidney 2) is a proximal tubular cell (PTC) line derived from normal kidney. 
The cells were immortalized by transduction with human papilloma virus 16 (HPV-16) E6/E7 genes. 
The recombinant retrovirus vector pLXSN 16 E6/E7 containing the HPV-16 E6/E7 genes was used to transfect the ectotropic packaging cell line Psi-2. 
Virus produced by the Psi-2 cells was used to infect the amphotropic packaging cell line PA317 (see ATCC CRL-9078). 
Virus produced by the PA317 cells was used to transduce primary PTCs. 
Although pLXSN 16 E6/E7 also confers resistance to neomycin, selection in G418 was not used to isolate transduced clones. 
The cell line appears to be derived from a single cell based on Southern and FISH analysis. 
The E6/E7 genes are present in the HK-2 genome as determined by PCR. 
The cells retain a phenotype indicative of well differentiated PTCs. 
They are positive for alkaline phosphatase, gamma glutamyltranspeptidase, leucine aminopeptidase, acid phosphatase, cytokeratin, alpha 3,beta 1 integrin, and fibronectin. 
The cells are negative for factor VIII related antigen, 6.19 antigen and CALLA endopeptidase. 
HK-2 cells retain functional characteristics of proximal tubular epithelium such as Na+ dependent / phlorizin sensitive sugar transport and adenylate cyclase responsiveness to parathyroid, but not to antidiuretic hormone. 
The cells are capable of gluconeogenesis as evidenced by their ability to make and store glycogen. 
HK-2 cells are anchorage dependent. 
The cells will not grow in methylcellulose, soft agar or suspension. 
HK-2 cells can reproduce experimental results obtained with freshly isolated PTCs.
Propagation:ATCC complete growth medium: The base medium for this cell line is provided by Invitrogen (GIBCO) as part of a kit: Keratinocyte Serum Free Medium (K-SFM), Kit Catalog Number 17005-042. This kit is supplied with each of the two additives required to grow this cell line (bovine pituitary extract (BPE) and human recombinant epidermal growth factor (EGF). To make the complete growth medium, you will need to add the following components to the base medium:
  • 0.05 mg/ml BPE - provided with the K-SFM kit
  • 5 ng/ml EGF - provided with the K-SFM kit. NOTE: Do not filter complete medium. 
    Atmosphere: air, 95%; carbon dioxide (CO2), 5% 
    Temperature: 37.0°C 
    Growth Conditions: Cell growth is dependent on epidermal growth factor. The cells should not be allowed to become confluent. Subculture at 80% of confluence.
Subculturing:Protocol:
  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 0.05% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
  3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
  5. To remove trypsin-EDTA solution, transfer cell suspension to centrifuge tube and spin at approximay 125 xg for 5 to 10 minutes.Discard supernatant and resuspend cells in fresh growth medium. Add appropriate aliquots of cell suspension to new culture vessels.
  6. Incubate cultures at 37°C.

會(huì)員登錄

X

請(qǐng)輸入賬號(hào)

請(qǐng)輸入密碼

=

請(qǐng)輸驗(yàn)證碼

收藏該商鋪

X
該信息已收藏!
標(biāo)簽:
保存成功

(空格分隔,最多3個(gè),單個(gè)標(biāo)簽最多10個(gè)字符)

常用:

提示

X
您的留言已提交成功!我們將在第一時(shí)間回復(fù)您~

以上信息由企業(yè)自行提供,信息內(nèi)容的真實(shí)性、準(zhǔn)確性和合法性由相關(guān)企業(yè)負(fù)責(zé),化工儀器網(wǎng)對(duì)此不承擔(dān)任何保證責(zé)任。

溫馨提示:為規(guī)避購買風(fēng)險(xiǎn),建議您在購買產(chǎn)品前務(wù)必確認(rèn)供應(yīng)商資質(zhì)及產(chǎn)品質(zhì)量。

撥打電話
在線留言
黑丝av少妇精品久久久久久久-中文字幕久久久人妻无码| 国产精品久久久精品一区-99久久免费精品国产男女性高好| 天天干天天日天天射天天舔-精品香蕉视频官网在线观看| 欧美精品国产白浆久久正在-国产精彩视频一区二区三区| 午夜福利卫生纸福利院-一区二区三区久久亚洲| 日本高清二区视频久二区-大香蕉在线视频大香蕉在线视频| 国产亚洲欧美一区91-亚洲欧美一区二区在线| 国产一级片久久免费看同-麻豆精品尤物一区二区青青| 97香蕉久久国产在线观看-麻豆黄色广告免费看片| mm在线精品视频在线观看-欧美国产日韩在线一区二区三区| 一区二区国产高清在线-日本高清无卡一区二区三区| 国产午夜精品理论片A级漫画-久久精品国产99亚洲精品| av中文字幕男人天堂-懂色av一区二区三区在线观看| 国产成人精品免费视频大全办公室-亚洲欧美日本综合在线| 国产高清av免费在线观看-黄片毛片大全一区二区三区| 国产精品成人欧美激情-黄色床上完整版高清无遮挡| 色综合色综合久久综合频道-埃及艳后黄版在线观看| 亚洲av综合av一区东京热-黄页免费视频网站在线观看| 少妇一区二区三区粉嫩av-国产精品区久久久久久久| 欧美伦乱淫老妇女激情吧-亚洲女邻居精品二区久久| 熟女熟妇伦51788-国产av在线播放一区二区三区| 亚洲视频一区二区三区免费-国产一级黄色大片在线| 国产精品久久99精品毛片-国产四季高清一区二区三区| 日韩二级视频在线观看-美女扒开奶罩露出奶子的视频网站| 99久热精品免费观看四虎-亚洲天堂精品视频在线| 交换朋友的妻子中文字幕-日本美女8x8x8x8| 亚洲国产日韩精品四区-dy888午夜福利精品国产97| 国模自慰一区二区三区-日韩一级黄色片天天看| 欧美精品国产系列一二三国产真人-在线观看国产午夜视频| 丝袜高跟熟女视频国产-熟女少妇亚洲一区二区| 欧美一级二级三级在线看-日韩精品欧美嫩草久久99| 91蜜桃传媒一二三区-日韩欧美国产一区呦呦| 欧美一区二区三区调教视频-三上悠亚国产精品一区二区三区| 黄色91av免费在线观看-欧美黄片一级在线观看| 国模自慰一区二区三区-日韩一级黄色片天天看| 欧美日韩国产综合新一区-国产综合av一区二区三区| 日韩综合精品一区二区-丝袜美腿熟女人妻经典三级| 国产一区二区三区四区五区麻豆-欧美一级在线视频播放| 国语自产偷拍精品视频偷拍-国产伊人这里只有精品视频| 午夜精品午夜福利在线-内射无套内射国产精品视频| 久久99热这里都是精品啊-国产成人亚洲精品无码aV|