欧美……一区二区三区,欧美日韩亚洲另类视频,亚洲国产欧美日韩中字,日本一区二区三区dvd视频在线

上海復(fù)祥生物科技有限公司

當(dāng)前位置:> 供求商機(jī)> CRL-2784-2V6.11 JHU-67 人胚腎細(xì)胞

[供應(yīng)]CRL-2784-2V6.11 JHU-67 人胚腎細(xì)胞

貨物所在地:上海上海市

產(chǎn)地:美國(guó)

更新時(shí)間:2024-04-01 16:25:20

有效期:2024年4月1日 -- 2025年4月1日

已獲點(diǎn)擊:169

聯(lián)系我時(shí),請(qǐng)告知來(lái)自 化工儀器網(wǎng)

CRL-2784 2V6.11 [JHU-67] 人胚腎細(xì)胞ATCC 細(xì)胞|細(xì)胞系|細(xì)胞株|腫瘤細(xì)胞|細(xì)胞,。細(xì)胞庫(kù)管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和*培養(yǎng)條件

CRL-2784 2V6.11 [JHU-67] 人胚腎細(xì)胞
ATCC® Number:  CRL-2784™     
Designations:  2V6.11 [JHU-67]  
Depositors:   G Ketner  
Biosafety Level: 2 [Cells containing Adenovirus viral DNA sequences ]  
Shipped:  frozen  
Medium & Serum:  See Propagation  
Growth Properties: adherent 
Organism: Homo sapiens (human)  
Morphology: epithelial

 CRL-2784 2V6.11 [JHU-67] 人胚腎細(xì)胞
Source: Organ: kidney 
Cell Type: epithelialtransformed with adenovirus 5 DNA 
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.  
 
Restrictions: Part of the Johns Hopkins Special Collection  
Isolation:  Isolation date: 2001 
Age:  fetus  
Comments: This line was derived from the human embryonic kidney line, 293 in 2001. 293 cells were transfected with the plasmid pVgRXR bearing a Zeocin-resistance selectable marker to obtain 293VgRXR cells. This population was subsequently transfected with the plasmid pEKORF6 containing Ad5 E4 ORF 6 that encodes E4 34K. pEKORF6 is a derivative of the plasmid pIndHydro that contains a hygromycin resistance gene. The vectors contain SV40 viral DNA sequences. The 2V6.11 cell line was originally selected in hygromycin-containing medium and cloned by single-colony isolation with a cloning cylinder. The 2V6.11 cells inducibly express the human adenovirus E4, 34kDa protein (E4 ORF6). 2V6.11 cells exhibit little or no constitutive E4 biologic activity. Induction by ponasterone A, an ecdysone analog, however, results in E4 biologic activity and levels of E4 34kDa protein that are detectable by immunoblotting. These cells can be used as a tool to study the biology of the adenoviral E4 oncoprotein. 
Propagation:  ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Atmosphere: air, 95%; carbon dioxide (CO2), 5% 
Temperature: 37.0°C 
Growth Conditions: The depositor recommends periodic hygromycin selection. 
Subculturing:  Protocol: 
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
An inoculum of 5 X 10(3) to 1 X 10(4) viable cells/cm2 is recommended. 
Incubate cultures at 37°C.

Interval: Maintain cultures at a cell concentration between 2 X 10(4) and 2 X 10(5) cells/cm2. 
Subc*tion Ratio: A subc*tion ratio of 1:6 to 1:10 is recommended 
Medium Renewal: Two to three times weekly 
Preservation:  Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO 
Storage temperature: liquid nitrogen vapor phase 
Doubling Time:  23 hrs.  
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003
recommended serum:ATCC 30-2020
source culture:ATCC JHU-67 
 Related Links   
NCBI Entrez Search  
Make a Deposit  
Frequently Asked Questions  
Material Transfer Agreement  
Technical Support  
Related Cell Culture Products  
 
 CRL-2784 2V6.11 [JHU-67] 人胚腎細(xì)胞

會(huì)員登錄

X

請(qǐng)輸入賬號(hào)

請(qǐng)輸入密碼

=

請(qǐng)輸驗(yàn)證碼

收藏該商鋪

X
該信息已收藏!
標(biāo)簽:
保存成功

(空格分隔,最多3個(gè),單個(gè)標(biāo)簽最多10個(gè)字符)

常用:

提示

X
您的留言已提交成功!我們將在第一時(shí)間回復(fù)您~

以上信息由企業(yè)自行提供,信息內(nèi)容的真實(shí)性、準(zhǔn)確性和合法性由相關(guān)企業(yè)負(fù)責(zé),化工儀器網(wǎng)對(duì)此不承擔(dān)任何保證責(zé)任。

溫馨提示:為規(guī)避購(gòu)買(mǎi)風(fēng)險(xiǎn),建議您在購(gòu)買(mǎi)產(chǎn)品前務(wù)必確認(rèn)供應(yīng)商資質(zhì)及產(chǎn)品質(zhì)量。

撥打電話
在線留言
国产精品二区高清在线-91精品91久久久久久| 亚洲国产视频不卡一区-激情欧美视频一区二区| 午夜福利卫生纸福利院-一区二区三区久久亚洲| 亚洲愉拍自拍欧美精品app-亚洲一区不卡在线视频| av中文字幕男人天堂-懂色av一区二区三区在线观看| 黑丝av少妇精品久久久久久久-中文字幕久久久人妻无码| 熟女熟妇伦51788-国产av在线播放一区二区三区| 青青操大香蕉在线播放-国产亚洲欧美精品在线观看| 国产欧美日韩中文字幕在线-国产伊人一区二区三区四区| 韩漫一区二区在线观看-精品国产免费未成女一区二区三区| 日韩精品亚洲不卡一区二区-成人网在线视频精品一区二区三区| 日韩亚洲欧美综合在线-成人在线网站在线观看| 亚洲欧美一区二区中文-台湾中文综合网妹子网| 亚洲欧美精品在线一区-99热国产在线手机精品99| 国产av剧情护士麻豆-三级国产精品欧美在线观看| 欧美精品国产系列一二三国产真人-在线观看国产午夜视频| 一区二区国产高清在线-日本高清无卡一区二区三区| 欧美日韩国产亚洲免费-肉体粗喘娇吟国产91| 亚洲另类熟女国产精品-懂色一区二区三区在线播放| 青青操视频在线观看国产-欧美成人乱码在线观看| 欧美日本亚一级二级三区久久精品-日韩欧美一区二区久久婷婷| 欧美精品国产白浆久久正在-国产精彩视频一区二区三区| 亚洲综合久久综合激情-日韩欧美精品人妻二区少妇| 久久影视av一区二区-人妻激情乱偷一区二区三区| 九九热这里只有精品九九-欧美日韩人妻精品一二三| 精品少妇一区二区18-一区二区三区日韩在线播放| 亚洲一区二区少妇激情-国产精品美女久久高潮| 五月婷婷免费观看视频-男人操女人下面视频在线免费看| 国产精品一区二区在线免费-久久精品国产亚洲av热明星| 国产欧美成人精品第一区-日本黄色精品一区二区| 久久精品亚洲无中文东京热-日本妹子内谢视频一区| 国产成人精品亚洲精品密奴-国产成人AV无码精品| 国产老熟女激情小视频-成人一区二区人妻不卡视频| 中文字幕偷拍亚洲九色-亚洲视频不卡一区二区天堂| 午夜福利1区2区3区-午夜洗澡免费视频网站| 97香蕉久久国产在线观看-麻豆黄色广告免费看片| 国产一级片久久免费看同-麻豆精品尤物一区二区青青| 午夜福利院免费在线观看-久久精品日产第一区二区三区画质| 国产成人精品亚洲精品密奴-国产成人AV无码精品| 麻豆久久国产精品亚洲-日本理论中文字幕在线视频| 国产一级片久久免费看同-麻豆精品尤物一区二区青青|