CRL-1993 HMy2.CIR 人B淋巴母細胞，ATCC 細胞|細胞系|細胞株|腫瘤細胞|細胞|貼壁細胞|懸浮細胞|；細胞庫管理規(guī)范，提供的細胞株背景清楚，提供參考文獻和*培養(yǎng)條件！
CRL-1993 HMy2.CIR 人B淋巴母細胞
| ATCC® Number: | CRL-1993™ | | |
| Designations: | HMy2.CIR [C1R, HMy2.C1R] | | Depositors: | P Cresswell | | Biosafety Level: | 2 [Cells contain Eptein-Barr Viral DNA sequences ] | | Shipped: | frozen | | Medium & Serum: | See Propagation | | Growth Properties: | suspension | | Organism: | Homo sapiens (human) | | Morphology: | lymphoblast
| | Source: | Cell Type: B lymphoblast; | | Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | | | | DNA Profile (STR): | Amelogenin: X
CSF1PO: 6,10
D13S317: 11,13
D16S539: 9,13
D5S818: 10,13
D7S820: 7,12
THO1: 8
TPOX: 8
vWA: 17 | | Comments: | The CRL-1621cell line is reported to be positive for Epstein-Barr nuclear antigen (EBNA +) and Epstein-Barr viral capsid antigen (EBVCA +). Since the HMy2.CIR cell line was derived from the HMy.2 B lymphoblastoid cell line, a fast growing mutant of the ARH-77 cell line (ATCC CRL-1621), it is assumed to be EBNA positive.
The HMy2.CIR cell line was derived from the HMy.2 B lymphoblastoid cell line, a fast growing mutant of the ARH-77 cell line (ATCC CRL-1621), by gamma irradiation and selection for loss of HLA class I antigen expression. [23312]
The cells express no HLA A or B locus products, but do express small amounts of HLA Cw4.
The cells are useful as host for transfected class I major histocompatibility antigen genes. | | Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Iscove's Modified Dulbecco's Medium, Catalog No. 30-2005. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C | | Subculturing: | Protocol: Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10(5) viable cells/ml.
Interval: Maintain between 2 X 10(5) and 1 X 10(6) viable cells/ml.
Medium Renewal: Every 2 to 3 days | | Preservation: | Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase | | Related Products: | parental cell line:ATCC CRL-1621
derivative:ATCC CRL-2369
derivative:ATCC CRL-2370
derivative:ATCC CRL-2371 | | References: | 23312: Storkus WJ, et al. Reversal of natural killing susceptibility in target cells expressing transfected class I HLA genes. Proc. Natl. Acad. Sci. USA 86: 2361-2364, 1989. PubMed: 2784569 |
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