欧美……一区二区三区,欧美日韩亚洲另类视频,亚洲国产欧美日韩中字,日本一区二区三区dvd视频在线

上海復(fù)祥生物科技有限公司

當(dāng)前位置:> 供求商機(jī)> CRL-11268-293T 人胚腎細(xì)胞

[供應(yīng)]CRL-11268-293T 人胚腎細(xì)胞

貨物所在地:上海上海市

產(chǎn)地:美國(guó)

更新時(shí)間:2024-04-01 16:25:19

有效期:2024年4月1日 -- 2025年4月1日

已獲點(diǎn)擊:115

聯(lián)系我時(shí),請(qǐng)告知來(lái)自 化工儀器網(wǎng)

CRL-11268 293T 人胚腎細(xì)胞ATCC 細(xì)胞|細(xì)胞系|細(xì)胞株|腫瘤細(xì)胞|細(xì)胞|貼壁細(xì)胞|懸浮細(xì)胞|。細(xì)胞庫(kù)管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和*培養(yǎng)條件

CRL-11268 293T 人胚腎細(xì)胞原代細(xì)胞
ATCC® Number:  CRL-11268™       
Designations:  293T/17 [HEK 293T/17] 
Depositors:   Rockefeller Univ.  
Biosafety Level: 2 [Cells contain Adeno and SV-40 viral DNA sequences ] 
Shipped:  frozen  
Medium & Serum:  See Propagation 
Growth Properties: adherent 
Organism: Homo sapiens (human) 
Morphology: epithelial

 CRL-11268 293T 人胚腎細(xì)胞
Source: Organ: kidney 
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. 
  
Restrictions: The line is available with the following restriction: 1. The cell line was deposited at the ATCC by Rockefeller University and is provided for research purposes only. Neither the cell line nor the products derived from it may be sold or used for commercial purposes. Nor can the cells be distributed to third parties for purposes of sale, or producing for sale, cells or their products. The cells are provided as a service to the research community. They are provided without warranty of merchantability or fitness for a particular purpose or any other warranty, expressed or implied. 2. Any proposed commercial use of the cells, or their products, must first be negotiated with Cell Genesys, 500 Forbes Boulevard, South San Francisco, CA 94080 Attn: Robert H. Tidwell; Senior Vice President, Corporate Development. 
Antigen Expression: SV40 T antigen [45408] 
Age:  fetus  
Comments: The 293T/17 cell line is a derivative of the 293T (293tsA1609neo) cell line. 293T is a highly transfectable derivative of the 293 cell line into which the temperature sensitive gene for SV40 T-antigen was inserted. 293T cells were cloned and the clones tested with the pBND and pZAP vectors to obtain a line capable of producing high titers of infectious retrovirus, 293T/17. These cells constitutively express the simian virus 40 (SV40) large T antigen, and clone 17 was selected specifically for its high transfectability.293T/17 cells were cotransfected with the pCRIPenv- and the pCRIPgag-2 vectors to obtain the ANJOU 65 (see ATCC CRL-11269) cell line.ANJOU 65 cells were cotransfected with the pCRIPgag-2 and pGPT2E vectors to obtain the BOSC 23 (see ATCC CRL-11270) ecotropic envelope-expression packaging cell line.ANJOU 65 cells were also cotransfected with the pCRIPAMgag vector along with a plasmid expressing the gpt resistance gene to obtain the Bing (see ATCC CRL-11554) amphotropic envelope-expression packaging cell line. 
Propagation:  ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C CRL-11268 293T 人胚腎細(xì)胞
Subculturing:  Protocol: 
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.

Subc*tion Ratio: A subc*tion ratio of 1:4 to 1:8 is recommended 
Medium Renewal: Every 2 to 3 days 
Preservation:  Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO 
Storage temperature: liquid nitrogen vapor phase 
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
recommended serum:ATCC 30-2020
derivative:ATCC CRL-11269 
References: 45408: Sena-Esteves M, et al. Single-step conversion of cells to retrovirus vector producers with herpes simplex virus-Epstein-Barr virus hybrid amplicons. J. Virol. 73: 10426-10439, 1999. PubMed: 10559361
57446: Pensiero M, et al. Retroviral vectors produced by producer cell lines resistant to lysis by human serum. US Patent 5,952,225 dated Sep 14 1999
57447: Pensiero M, et al. Retroviral vectors produced by producer cell lines resistant to lysis by human serum. US Patent 6,329,199 dated Dec 11 2001
57448: Pear WS, et al. Production of High-Titer Helper-Free Retroviruses by Transient Transfection. Proc. Natl. Acad. Sci. USA 90: 8392-8396, 1993. PubMed: 7690960 
 

會(huì)員登錄

X

請(qǐng)輸入賬號(hào)

請(qǐng)輸入密碼

=

請(qǐng)輸驗(yàn)證碼

收藏該商鋪

X
該信息已收藏!
標(biāo)簽:
保存成功

(空格分隔,最多3個(gè),單個(gè)標(biāo)簽最多10個(gè)字符)

常用:

提示

X
您的留言已提交成功!我們將在第一時(shí)間回復(fù)您~

以上信息由企業(yè)自行提供,信息內(nèi)容的真實(shí)性、準(zhǔn)確性和合法性由相關(guān)企業(yè)負(fù)責(zé),化工儀器網(wǎng)對(duì)此不承擔(dān)任何保證責(zé)任。

溫馨提示:為規(guī)避購(gòu)買(mǎi)風(fēng)險(xiǎn),建議您在購(gòu)買(mǎi)產(chǎn)品前務(wù)必確認(rèn)供應(yīng)商資質(zhì)及產(chǎn)品質(zhì)量。

撥打電話
在線留言
国产精品一区二区在线免费-久久精品国产亚洲av热明星| 亚洲国产视频不卡一区-激情欧美视频一区二区| sobo欧美在线视频-免费av网址一区二区| 午夜精品午夜福利在线-内射无套内射国产精品视频| 蜜臀一区二区三区精品在线-99久久久精品免费看国产| 国产aa视频一区二区三区-国产精品久久久久久久毛片动漫| 熟妇勾子乱一区二区三区-欧美爱爱视频一区二区| 亚洲欧美日韩国产一区二区三区-国产欧美日韩一区二区免费| 蜜臀一区二区三区精品在线-99久久久精品免费看国产| 中文字幕日韩精品不卡一区二区-成人av在线观看一区二区| 白嫩美女娇喘呻吟高潮-久久一区二区三区日产精品| 亚洲国产精品日韩欧美-国产又粗又硬又大爽黄| 青青草原免费国产在线视频-精品人妻乱码一区二区三区四区| 婷婷亚洲欧美综合丁香亚洲-超刺激国语对白在线视频| 中文字幕亚洲综合久久最新-久久精品视频免费久久久| av中文字幕男人天堂-懂色av一区二区三区在线观看| 国产欧美日本不卡精美视频-日本后入视频在线观看| 国产在线一区二区三区欧美-久久偷拍精品视频久久| 中文字幕亚洲中文字幕-丰满老妇伦子交尾在线播放| 人妻丝袜中文字幕在线视频-亚洲成av人片一区二区三区| 哦啊好大用力欧美视频-麻豆国产传媒片在线观看| 激情字幕久久久字幕中文-一区二区三区免费黄片| 日韩高清在线观看一区二区-美产av在线免费观看| 99在线观看精品视频免费-国产极品一区二区三区四区| 日本亚洲精品中字幕日产2020-很黄很黄的裸交视频网站| 欧美精品国产白浆久久正在-国产精彩视频一区二区三区| 成人av一区二区蜜桃-亚洲色图激情人妻欧美| 俄罗斯胖老太太黄色特级片-国产精品黑丝美腿美臀| 亚洲欧洲成视频免费观看-国产福利一区二区在线观看| 一区二区三区国产高清mm-美女张开腿让帅哥桶爽| 亚洲另类熟女国产精品-懂色一区二区三区在线播放| 精品一区二区三区av在线-欧美黑人巨大精品一区二区| 97一区二区三区在线-欧美护士性极品hd4k| 日韩精品一区二区三区十八-日韩人妻少妇一区二区三区| 国产黄污网站在线观看-成人av电影中文字幕| 日韩中文字幕v亚洲中文字幕-日韩亚洲av免费在线观看| 18禁真人在线无遮挡羞免费-中文字幕精品一区二区三区四区| 欧美日本亚一级二级三区久久精品-日韩欧美一区二区久久婷婷| 国产在线不卡高清一区-日本一区二区三区四区无卡| av一区免费在线观看-中文字幕日韩国产精品视频| 中文字幕人妻少妇第一页-隔壁的女孩在线看中文字幕|