欧美……一区二区三区,欧美日韩亚洲另类视频,亚洲国产欧美日韩中字,日本一区二区三区dvd视频在线

上海復(fù)祥生物科技有限公司

當(dāng)前位置:> 供求商機(jī)> CRL-11610-RWPE-2 人前列腺正常細(xì)胞

[供應(yīng)]CRL-11610-RWPE-2 人前列腺正常細(xì)胞

貨物所在地:上海上海市

產(chǎn)地:美國(guó)

更新時(shí)間:2024-04-18 10:42:09

有效期:2024年4月18日 -- 2025年4月18日

已獲點(diǎn)擊:135

聯(lián)系我時(shí),請(qǐng)告知來(lái)自 化工儀器網(wǎng)

CRL-11610 RWPE-2 人前列腺正常細(xì)胞,原代細(xì)胞|細(xì)胞系|細(xì)胞株|菌種;細(xì)胞庫(kù)管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和*培養(yǎng)條件!

CRL-11610 RWPE-2 人前列腺正常細(xì)胞

ATCC® Number:CRL-11610™    Price: 
Designations:RWPE-2
Depositors: Michigan State University, National Cancer Institute
Biosafety Level:2 [Cells contain Human Papilloma viral (HPV) sequences ]
Shipped:frozen
Medium & Serum:See Propagation
Growth Properties:adherent
Organism:Homo sapiens (human)
Morphology:CRL-11610 RWPE-2 人前列腺正常細(xì)胞epithelial
 
Source:Organ: prostate 
Disease: normal 
Cell Type: epithelial
Cellular Products:cytokeratin 18 [46793] 
cytokeratin 8 [46793]
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
 
Receptors:androgen receptor, expressed ( [46793] upregulated upon exposure to androgen)
Tumorigenic:Yes
Antigen Expression:kallikrein 3, KLK3 (prostate specific antigen, PSA); Homo sapiens, expressed (upon exposure to androgen) ( [46793] upon exposure to androgen)
DNA Profile (STR):Amelogenin: X,Y 
CSF1PO: 13 
D13S317: 8,14 
D16S539: 9,11 
D5S818: 12,15 
D7S820: 10,11 
THO1: 8,9.3 
TPOX: 8,11 
vWA: 14,18
Cytogenetic Analysis:At passage 14, a majority of the cells were in the near diploid range (48-54), with the main population being 51, XY. [46795]
Isoenzymes:AK-1, 1 
ES-D, 2 
G6PD, B 
GLO-I, 1-2 
Me-2, 0 
PGM1, 2 
PGM3, 1
Gender:CRL-11610 RWPE-2 人前列腺正常細(xì)胞male
Ethnicity:Caucasian, White
Comments:Tumor Suppressor Gene(s):pRB + [PubMed: 9214605]
RWPE-2 cells were derived from RWPE-1 cells (ATCC CRL-11609) by transformation with Ki-ras using the Kirsten murine sarcoma virus (Ki-MuSV) [PubMed: 9214605]. Epithelial cells from the peripheral zone of a histologically normal adult human prostate were transfected with a plasmid carrying one copy of the human papilloma virus 18 (HPV-18) genome to establish the RWPE-1 cell line (ATCC CRL-11609). RWPE-1 cells neither grow in agar nor form tumors when injected into nude mice with or without Matrigel. However, RWPE-2 cells form small colonies in soft agar and form tumors when injected into nude mice [PubMed: 9214605]. Further, a family of tumorigenic cell lines, that mimics multiple steps in prostate cancer progression, was also derived from RWPE-1 cells by exposure to N-methyl-N-nitrsourea (MNU). See the WPE1-NA22 (ATCCCRL-2849), WPE1-NB14 (ATCC CRL-2850), WPE1-NB11 (ATCC CRL-2851) and WPE1-NB26 (ATCC CRL-2852) cell ines.
The depositor reports that the RWPE-1 cell line (ATCC CRL-11609) was screened, and found negative for, Hepatitis B virus, Hepatitis C virus and Human immunodeficiency virus.
Propagation:ATCC complete growth medium: The base medium for this cell line is provided by Invitrogen (GIBCO) as part of a kit: Keratinocyte Serum Free Medium (K-SFM), Kit Catalog Number 17005-042. This kit is supplied with each of the two additives required to grow this cell line (bovine pituitary extract (BPE) and human recombinant epidermal growth factor (EGF). To make the complete growth medium, you will need to add the following components to the base medium:
  • 0.05 mg/ml BPE - provided with the K-SFM kit
  • 5 ng/ml EGF - provided with the K-SFM kit. NOTE: Do not filter complete medium. 
    Atmosphere: air, 95%; carbon dioxide (CO2), 5% 
    Temperature: 37.0°C
Subculturing:CRL-11610 RWPE-2 人前列腺正常細(xì)胞Protocol:
  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with Ca++/Mg++ free Dulbecco's phosphate-buffered saline (D-PBS).
  3. Add 2.0 to 3.0 ml (to a T-25 flask) or 3.0 to 4.0 ml (to a T-75 flask) of 0.05% Trypsin - 0.53mM EDTA solution, diluted 1:1 with D-PBS, and place flask in a 37C incubator for 5 to 8 minutes. Observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 10 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach.
  4. Add 6.0 to 8.0 ml of 0.1% Soybean Trypsin Inhibitor (or 2% fetal bovine serum in D-PBS), as appropriate, and aspirate cells by gently pipetting.
  5. Transfer cell suspension to centrifuge tube and spin at approximay 125 x g for 5 to 7 minutes.
  6. Discard supernatant and resuspend cells in fresh serum-free growth medium. Add appropriate aliquots of cell suspension to new culture vessels. An inoculum of 2 X 10(4) to 4 X 10(4) viable cells/sq. cm is recommended.
  7. Incubate cultures at 37C. We recommend that you maintain cultures at a cell concentration between 4 X 10(4) and 8 X 10(4) cells/sq. cm.
Cells grown under serum-free or reduced serum conditions may not attach strongly during the 24 hours after subculture and should be disturbed as little as possible during that period. 
Subc*tion Ratio: A subc*tion ratio of 1:3 to 1:5 is recommended 
Medium Renewal: Every 2 days
Preservation:Freeze medium: Complete growth medium supplemented with 10% (v/v) DMSO 
Storage temperature: liquid nitrogen vapor phase
Related Products:parental cell line:ATCC CRL-11609
purified DNA:ATCC CRL-11610D
derived from same individual:ATCC CRL-2849
derived from same individual:ATCC CRL-2850
derived from same individual:ATCC CRL-2852
derived from same individual:ATCC CRL-2851
derivative:ATCC CRL-2853
derived from same individual:ATCC CRL-2854
References:46792: Webber MM, Rhim JS. Immortalized and malignant human prostatic cell lines. US Patent 5,824,488 dated Oct 20 1998
46793: Bello D, et al. Androgen responsive adult human prostatic epithelial cell lines immortalized by human papillomavirus 18. Carcinogenesis 18: 1215-1223, 1997. PubMed: 9214605
46795: Webber MM, et al. Acinar differentiation by non-malignant immortalized human prostatic epithelial cells and its loss by malignant cells. Carcinogenesis 18: 1225-1231, 1997. PubMed: 9214606
53180: Webber MM, et al. Immortalized and tumorigenic adult human prostatic epithelial cell lines: characteristics and applications. Part I. Cell markers and immortalized nontumorigenic cell lines. Prostate 29: 386-394, 1996. PubMed: 8977636
53181: Webber MM, et al. Immortalized and tumorigenic adult human prostatic epithelial cell lines: characteristics and applications Part 2. Tumorigenic cell lines. Prostate 30: 58-64, 1997. PubMed: 9018337
53182: Webber MM, et al. Immortalized and tumorigenic adult human prostatic epithelial cell lines: characteristics and applications. Part 3. Oncogenes, suppressor genes, and applications. Prostate 30: 136-142, 1997. PubMed: 9051152
53183: Kremer R, et al. ras Activation of human prostate epithelial cells induces overexpression of parathyroid hormone-related peptide. Clin. Cancer Res. 3: 855-859, 1997. PubMed: 9815759
53192: Jacob K, et al. Osteonectin promotes prostate cancer cell migration and invasion: a possible mechanism for metastasis to bone. Cancer Res. 59: 4453-4457, 1999. PubMed: 10485497
CRL-11610 RWPE-2 人前列腺正常細(xì)胞

會(huì)員登錄

X

請(qǐng)輸入賬號(hào)

請(qǐng)輸入密碼

=

請(qǐng)輸驗(yàn)證碼

收藏該商鋪

X
該信息已收藏!
標(biāo)簽:
保存成功

(空格分隔,最多3個(gè),單個(gè)標(biāo)簽最多10個(gè)字符)

常用:

提示

X
您的留言已提交成功!我們將在第一時(shí)間回復(fù)您~

以上信息由企業(yè)自行提供,信息內(nèi)容的真實(shí)性、準(zhǔn)確性和合法性由相關(guān)企業(yè)負(fù)責(zé),化工儀器網(wǎng)對(duì)此不承擔(dān)任何保證責(zé)任。

溫馨提示:為規(guī)避購(gòu)買(mǎi)風(fēng)險(xiǎn),建議您在購(gòu)買(mǎi)產(chǎn)品前務(wù)必確認(rèn)供應(yīng)商資質(zhì)及產(chǎn)品質(zhì)量。

撥打電話
在線留言
亚洲一区二区免费av-中文字幕人妻久久久一区二区三区| 亚洲欧美日韩二区三区-国产在线欧美一区日韩二区| 国产精品成人欧美激情-黄色床上完整版高清无遮挡| 国产在线一区二区三区欧美-久久偷拍精品视频久久| av噜噜国产在线观看-欧美视频亚洲视频一区二区三区| 国产小黄片高清在线观看-涩涩鲁精品亚洲一区二区| 小12萝自慰喷水亚洲网站-chinese偷拍一区二区三区| 色噜噜噜噜一区二区三区-欧美最猛黑人做爰视频| 亚洲另类熟女国产精品-懂色一区二区三区在线播放| 亚洲国产中文欧美一区二区三区-国产精品一区二区视频成人| 三级a级一级大片在线观看-日韩av有码免费观看| 欧美日韩国产在线资源-超碰成人国产一区二区三区| 婷婷六月视频在线观看-久久亚洲综合国产精品| 两性污污视频网站在线观看-亚洲欧美日韩激情一区| 日本高清二区视频久二区-大香蕉在线视频大香蕉在线视频| 欧美日韩成人在线观看-久久五月婷婷免费视频| 亚洲欧美一区二区中文-台湾中文综合网妹子网| 亚洲av专区在线观看国产-丰满人妻av一区二区三区| 久久精品亚洲国产av久-国产精品视频一区二区免费| 哦啊好大用力欧美视频-麻豆国产传媒片在线观看| 久久精品亚洲国产av久-国产精品视频一区二区免费| 国产精品v欧美精品v日韩精品-国产欧美日韩精品区一区二污污污| 欧美日韩亚洲1区2区-黄污视频在线观看不卡| 久久精品国产96精品-日韩人成理论午夜福利| av噜噜国产在线观看-欧美视频亚洲视频一区二区三区| 久色高清精品在线国产-国产精品视频一区三区四区| 亚洲一区二区三在线观看-国产精品亚洲а∨天堂123| 国产一区二区三区四区五区麻豆-欧美一级在线视频播放| 国产美女裸露无遮挡双奶网站-国产精品色午夜视频免费看| 日本高清二区视频久二区-大香蕉在线视频大香蕉在线视频| 中文字幕亚洲中文字幕-丰满老妇伦子交尾在线播放| 日韩精品一区二区三区粉嫩av-欧美亚洲国产中文字幕| 亚洲一区二区少妇激情-国产精品美女久久高潮| 国产小黄片高清在线观看-涩涩鲁精品亚洲一区二区| 欧美一区二区三区调教视频-三上悠亚国产精品一区二区三区| 欧美精品国产系列一二三国产真人-在线观看国产午夜视频| 99久久精品一区二区成人-麻豆国产av玩弄放荡人妇系列| 亚洲精品蜜桃在线观看-国产欧美日韩在线观看精品观看| 午夜精品午夜福利在线-内射无套内射国产精品视频| 人妻少妇无乱码中文字幕-人成免费视频一区二区| 欧美日韩国产综合四区-爆操极品尤物熟妇14p|