欧美……一区二区三区,欧美日韩亚洲另类视频,亚洲国产欧美日韩中字,日本一区二区三区dvd视频在线

上海復祥生物科技有限公司

當前位置:> 供求商機> CCL-171-MRC-5 人胚肺細胞

[供應]CCL-171-MRC-5 人胚肺細胞

貨物所在地:上海上海市

產(chǎn)地:美國

更新時間:2024-04-18 10:31:54

有效期:2024年4月18日 -- 2025年4月18日

已獲點擊:113

聯(lián)系我時,請告知來自 化工儀器網(wǎng)

CCL-171 MRC-5 人胚肺細胞,原代細胞|細胞系|細胞株|菌種;細胞庫管理規(guī)范,提供的細胞株背景清楚,提供參考文獻和*培養(yǎng)條件!

CCL-171 MRC-5 人胚肺細胞
ATCC® Number:  CCL-171™        
Designations:  MRC-5  
Depositors:   JP Jacobs  
Biosafety Level: 1  
Shipped:  frozen  
Medium & Serum:  See Propagation  
Growth Properties: adherent 
Organism: Homo sapiens (human)  
Morphology: fibroblast

 
Source: Organ: lung 
Disease: normal 
Cell Type: fibroblast 
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.  
 
Isolation:  Isolation date: September, 1966 
Applications: efficacy testing [92587] 
testing [92346] [92389] 
transfection host (Roche FuGENE® Transfection Reagents)
viruscide testing [92579] [92582] 
Virus Susceptibility: poliovirus 1; herpes simplex; vesicular stomatitis (Indiana) 
Reverse Transcript: negative  
DNA Profile (STR): Amelogenin: X,Y 
CSF1PO: 11,12 
D13S317: 11,14 
D16S539: 9,11 
D5S818: 11,12 
D7S820: 10,11 
THO1: 8 
TPOX: 8 
vWA: 15 
Cytogenetic Analysis: Chromosome Frequency Distribution 50 Cells: 2n = 46. This is a normal diploid human cell line with 46,XY karyotype. The modal chromosome number was 46, occurring in 70% of cells. The rate of polyploidy was 3.6%. Both X and Y chromosomes were normal. 
Isoenzymes:  G6PD, B 
Age:  14 weeks gestation  
Gender:  male  
Ethnicity:  Caucasian  
Comments: The MRC-5 cell line was derived from normal lung tissue of a 14-week-old male fetus by J.P. Jacobs in September of 1966. 
The cells are capable of 42 to 46 population doublings before the onset of senescence. 
Propagation:  ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C 
Atmosphere: air, 95%; carbon dioxide (CO2), 5% 
Subculturing:  Protocol: 
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.

會員登錄

X

請輸入賬號

請輸入密碼

=

請輸驗證碼

收藏該商鋪

X
該信息已收藏!
標簽:
保存成功

(空格分隔,最多3個,單個標簽最多10個字符)

常用:

提示

X
您的留言已提交成功!我們將在第一時間回復您~

以上信息由企業(yè)自行提供,信息內容的真實性、準確性和合法性由相關企業(yè)負責,化工儀器網(wǎng)對此不承擔任何保證責任。

溫馨提示:為規(guī)避購買風險,建議您在購買產(chǎn)品前務必確認供應商資質及產(chǎn)品質量。

撥打電話
在線留言
人妻少妇无乱码中文字幕-人成免费视频一区二区| 久久精品亚洲国产av久-国产精品视频一区二区免费| 久久高清超碰av热热久久-国产高清不卡免费视频| 99久久精品一区二区成人-麻豆国产av玩弄放荡人妇系列| 国产av一区二区三区日韩接吻-av网址在线播放网站| 三上悠亚免费观看在线-青青草原在线视频观看精品| 男人的天堂久久精品激情-最新亚洲精品a国产播放| 国产精品久久99精品毛片-国产四季高清一区二区三区| 在线观看中午中文乱码-2021国产一级在线观看| 亚洲区欧美区在线视频-亚洲碰碰人人AV熟女天堂| 精品老熟妇一区二区三区-日韩丰满一区二区三区| 一级特黄大片亚洲高清-国产精品视频伊人久久| 午夜福利院免费在线观看-久久精品日产第一区二区三区画质| 91亚洲美女视频在线-熟妇人妻精品一区二区三区蜜臀| 91蜜桃传媒一二三区-日韩欧美国产一区呦呦| 国产一区二区无套内射-国内精品久久久久久久齐pp| 国产精品久久久精品一区-99久久免费精品国产男女性高好| 国产午夜精品理论片A级漫画-久久精品国产99亚洲精品| 久久久国产精品电影片-精品孕妇人妻一区二区三区| 亚洲av成人一区国产精品网-国产偷_久久一级精品a免费| 亚洲黄片三级三级三级-国产成人一区二区在线视频| 国产传媒中文字幕在线观看-午夜福利视频在线播放观看| 麻豆久久国产精品亚洲-日本理论中文字幕在线视频| 国产精品二区高清在线-91精品91久久久久久| 国产精品熟女视频一区二区-国产日韩精品欧美一区喷水| 国产av剧情护士麻豆-三级国产精品欧美在线观看| 欧美精品午夜一二三区-a屁视频一区二区三区四区| 可以免费看污污视频的网站-日韩欧美不卡视频在线观看| 欧美字幕一区二区三区-好吊妞欧美一区二区在线观看| 欧美aa一级视频播放-久一一区二区三区大香蕉| 黄片黄片在线免费观看-激情综合网激情五月俺也去| 亚洲女人黄色录像一区-日韩av电影在线免费看| 男女做爰猛烈啪啪吃奶在线观看-人妻连裤丝袜中文字幕| 蜜臀一区二区三区精品在线-99久久久精品免费看国产| 在线免费观看黄片喷水-国产精品白丝网站在线观看| 国产精品久久久精品一区-99久久免费精品国产男女性高好| 国产午夜精品理论片A级漫画-久久精品国产99亚洲精品| 亚洲欧美另类综合偷拍-婷婷社区综合在线观看| 日本少妇激情一区二区-亚洲自偷自拍熟女另类蜜臀| 久久精品亚洲无中文东京热-日本妹子内谢视频一区| 亚洲精品激情一区二区-久久成人国产欧美精品一区二区|