CL-101 LLC-PK1 豬腎細(xì)胞,ATCC 細(xì)胞|細(xì)胞系|細(xì)胞株|腫瘤細(xì)胞|細(xì)胞|貼壁細(xì)胞|懸浮細(xì)胞|,細(xì)胞庫(kù)管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和*培養(yǎng)條件
CL-101 LLC-PK1 豬腎細(xì)胞,ATCC 細(xì)胞|細(xì)胞系|細(xì)胞株|腫瘤細(xì)胞|細(xì)胞|貼壁細(xì)胞|懸浮細(xì)胞|,細(xì)胞庫(kù)管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和*培養(yǎng)條件
CL-101 LLC-PK1 豬腎細(xì)胞 的詳細(xì)介紹
ATCC® Number: | CL-101™ | Price: | |
Designations: | LLC-PK1 | Depositors: | Eli Lilly & Co. | Biosafety Level: | 1 | Shipped: | frozen | Medium & Serum: | See Propagation | | | Organism: | Sus scrofa (pig) | Morphology: | epithelial | Source: | Organ: kidney Strain: Hampshire Disease: normal | Cellular Products: | plasminogen activator | Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | | Age: | 3 to 4 weeks | Gender: | male | Propagation: | ATCC complete growth medium: The base medium for this cell line is Medium 199 containing 1.5 g/L sodium bicarbonate. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 3%. Temperature: 37.0°C | Subculturing: | Subc*tion Ratio: A subc*tion ratio of 1:3 to 1:8 is recommended Medium Renewal: Twice per week Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks. | Preservation: | culture medium 95%; DMSO, 5% | Related Products: | recommended serum:ATCC 30-2020 formerly distributed as:ATCC CRL-1392 | References: | 3520: Hull RN, Huseby RM. Enhanced production of plasminogen activator. US Patent 3,904,480 dated Sep 9 1975 22659: Perantoni A, Berman JJ. Properties of Wilms' tumor line (TuWi) and pig kidney line (LLC-PK1) typical of normal kidney tubular epithelium. In Vitro 15: 446-454, 1979. PubMed: 225262 28301: Loffler S, et al. CD9, a tetraspan transmembrane protein, renders cells susceptible to canine distemper virus. J. Virol. 71: 42-49, 1997. PubMed: 8985321 |
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