欧美……一区二区三区,欧美日韩亚洲另类视频,亚洲国产欧美日韩中字,日本一区二区三区dvd视频在线

上海復(fù)祥生物科技有限公司

當(dāng)前位置:> 供求商機(jī)> CRL-1651 Cos-7 非洲綠猴sv40轉(zhuǎn)化的腎細(xì)胞

[供應(yīng)]CRL-1651 Cos-7 非洲綠猴sv40轉(zhuǎn)化的腎細(xì)胞

貨物所在地:上海上海市

更新時間:2024-05-11 13:42:19

有效期:2024年5月11日 -- 2025年1月4日

已獲點(diǎn)擊:214

聯(lián)系我時,請告知來自 化工儀器網(wǎng)

CRL-1651 Cos-7 非洲綠猴sv40轉(zhuǎn)化的腎細(xì)胞,ATCC 細(xì)胞|細(xì)胞系|細(xì)胞株|腫瘤細(xì)胞|細(xì)胞|貼壁細(xì)胞|懸浮細(xì)胞|,細(xì)胞庫管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和*培養(yǎng)條件

CRL-1651 Cos-7 非洲綠猴sv40轉(zhuǎn)化的腎細(xì)胞,ATCC 細(xì)胞|細(xì)胞系|細(xì)胞株|腫瘤細(xì)胞|細(xì)胞|貼壁細(xì)胞|懸浮細(xì)胞|,細(xì)胞庫管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和*培養(yǎng)條件

CRL-1651 Cos-7 非洲綠猴sv40轉(zhuǎn)化的腎細(xì)胞 的詳細(xì)介紹

 

ATCC® Number:CRL-1651™   Price:$269.00
Designations:COS-7
Depositors: Y Gluzman
Biosafety Level:2 [Cells Contain SV-40 viral DNA sequences ]
Shipped:frozen
Medium & Serum:See Propagation
Growth Properties:adherent
Organism:Cercopithecus aethiops
Morphology:fibroblast
Source:Organ: kidney
Cell Type: SV40 transformed
Cellular Products:T antigen      
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
 
Applications:transfection host (Nucleofection technology from Lonza
Roche FuGENE® Transfection Reagents)
Virus Susceptibility:SV40 (lytic growth); SV40 tsA209 at 40C; SV40 mutants with deletions in the early region
Comments:This is an African green monkey kidney fibroblast-like cell line suitable for transfection by vectors requiring expression of SV40 T antigen. This line contains T antigen, retains complete permissiveness for lytic growth of SV40, supports the replication of ts A209 virus at 40C, and supports the replication of pure populations of SV40 mutants with deletions in the early region.The line was derived from the CV-1 cell line (ATCC ® CCL-70?) by transformation with an origin defective mutant of SV40 which codes for wild type T antigen.
Propagation:ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C          
Subculturing:Protocol:
  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
  3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
  5. Add appropriate aliquots of the cell suspension to new culture vessels.
  6. Incubate cultures at 37°C.

  

會員登錄

X

請輸入賬號

請輸入密碼

=

請輸驗(yàn)證碼

收藏該商鋪

X
該信息已收藏!
標(biāo)簽:
保存成功

(空格分隔,最多3個,單個標(biāo)簽最多10個字符)

常用:

提示

X
您的留言已提交成功!我們將在第一時間回復(fù)您~

以上信息由企業(yè)自行提供,信息內(nèi)容的真實(shí)性、準(zhǔn)確性和合法性由相關(guān)企業(yè)負(fù)責(zé),化工儀器網(wǎng)對此不承擔(dān)任何保證責(zé)任。

溫馨提示:為規(guī)避購買風(fēng)險,建議您在購買產(chǎn)品前務(wù)必確認(rèn)供應(yīng)商資質(zhì)及產(chǎn)品質(zhì)量。

撥打電話
在線留言
91麻豆精品人妻系例| 欧美亚洲另类久久综合| 亚洲美女被黑人疯狂进入| 97色欧美视频在线观看| 精品女厕一区二区三区| 后入美臀少妇一区二区| 亚洲欧美另类国产成人| 成人午夜国产一区二区| 人妻被粗大的猛烈进出| 中文岳妇荡欲丰满肥熟| 国产美女都是黄色的视频| 伊人五月开心六月丁香| 亚洲精品亚洲精亚洲精品| 欧美高清一区二区三区精品| 精品一区二区三区水蜜桃| 精品国产av一区二区三| 色综合91精品中文字幕| 亚洲熟女精品区一区二三| 国产91高潮流白浆在线麻豆| 三级国产视频在线观看| 国产成人av网址在线观看| 一区二区三区精品人妻18| 国产人妻一区二区三区四区| 免费的久久久精品视频| 中文字幕av一区二区三区高清| 国产精品福利片免费视频| 综合一区中文字幕乱码| 一区二区三区久久国产精品| 黑人与中国少妇内射视频| 国产亚洲精品久久麻豆| 成人一区二区免费视频| 九色综合一区二区三区| 免费的久久久精品视频| 91精品国产综合久久不乱| 一区二区三区久久伊人| 欧美日韩国产另类求看| 日韩国产欧美一区二区三区| 久国产精品一区国产精品| 免费久久精品麻豆一区二区av| 国产白丝叫黄在线观看| av大片一区二区三区|