美國trinity成年人百日咳桿菌IgM免疫診斷試紙
【簡單介紹】
【詳細說明】
成年人百日咳桿菌IgM免疫診斷試紙
英文名稱:Bordela Pertussis IgM (quant.)
廣州健侖生物科技有限公司
(廣州健侖生物科技有限公司是集研制開發(fā)、銷售、服務于一體的優(yōu)良企業(yè),公司產(chǎn)品涉及臨床快速診斷試劑、食品安全檢測試劑,違禁品快速檢測,動物疾病防疫檢測試劑,免疫診斷試劑、臨床血液學和體液學檢驗試劑、微生物檢驗試劑、分子生物學檢驗試劑、臨床生化試劑、有機試劑等眾多領域,同時核心代理Panbio、FOCUS、Qiagen、IBL、CORTEZ、Fuller、Inbios、BinaxNOW、LumuQuick、日本富士、日本生研等多家有名診斷產(chǎn)品集團公司產(chǎn)品,致力于為商檢單位、疾病預防控制中心、海關出入境檢疫局、衛(wèi)生防疫單位,緝毒系統(tǒng),戒毒中心,檢驗檢疫單位、生化企業(yè)、科研院所、醫(yī)療機構等機構與行業(yè)提供*、高品質的產(chǎn)品服務。此外,本公司還開展食品、衛(wèi)生、環(huán)境、藥品等多方面的第三方檢測服務。)
主要用途:用于定量測定人血清、腦脊液或血漿中的百日咳抗體。
產(chǎn)品規(guī)格:96T/盒
存儲條件:4-8℃
保質期:18個月
【百日咳癥狀】
Early symptoms: cough, sneeze, runny nose, tearing, low or moderate fever, 3 to 4 days after the symptoms disappear, heat back, but the cough gradually increased, especially at night as heavy. This period of the most contagious, sustainable 7 to 10 days, if treated promptly, can effectively control the development of the disease.
Late symptoms: uncontrollable, there will be paroxysmal spasmodic cough, intensified once and for all, until the cough a lot of viscous sputum and vomiting stomach contents only. Spasm cough predisposing seizures often throat itch, chest tightness and other illnesses. . Infants due to the glottis may be due to vocal cord spasm and congestion blocked the occurrence of apnea due to hypoxia and cyanosis occurred, or even convulsions, can also be due to suffocation and death.
【檢測原理】
用抗原包被微量板孔,制成固相載體。加患者血清到板孔中,其所含的抗體特異性地與固相載體中現(xiàn)存抗原結合,形成免疫復合物。除去多余物質后,加入結合了堿性磷酸酶的IgG、IgA或IgM抗體,使之與上述免疫復合物反應。洗板,除去多余的結合物,加入底物(對硝基苯磷酸鹽)。其與酶結合的免疫復合物反應,產(chǎn)生有顏色產(chǎn)物,顏色強度與特異性抗體含量成正比。
成年人百日咳桿菌IgM免疫診斷試紙
【試劑盒的組成】
試劑盒組成 | IgG試劑盒 IgM試劑盒 IgA試劑盒 數(shù)量 / 容積 |
微孔條(此微孔條可拆下單獨使用,每條有8孔,共96孔,已經(jīng)包被了抗原) 1個微孔條框架 包被材料未被激活 | 12 12 12 |
標準血清(立即可用) 人血清溶于含蛋白的磷酸鹽緩沖液;抗HIV抗體、抗乙肝病毒(HBV)表面抗原和抗丙肝病毒(HCV)抗體均為陰性; 防腐劑:< 0.1% * 染色劑:紫紅色O | 2×2毫升 2×2毫升 2×2毫升 |
陰性對照血清(立即可用) 人血清溶于含蛋白的磷酸鹽緩沖液;抗HIV抗體、抗乙肝病毒(HBV)表面抗原和抗丙肝病毒(HCV)抗體均為陰性; 防腐劑:< 0.1% * 染色劑:里沙明綠 V | 1×2毫升 1×2毫升 1×2毫升 |
酶標記的抗人IgG, IgA, IgM (立即可用) 羊抗人IgG, IgA, IgM(多克隆),標記堿性磷酸酶后在蛋白穩(wěn)定劑中儲存 防腐劑: 0.01% 甲基異噻唑啉酮 0.01% 溴化硝基二堊烷 | 13毫升 13毫升 13毫升 |
濃縮洗液(可稀釋至1000毫升) 氯化鈉溶液,含吐溫20和30mM Tris 防腐劑: < 0.1%* | 1×33.3毫升 1×33.3毫升 1×33.3毫升 |
稀釋緩沖液 磷酸鹽緩沖液,內(nèi)含蛋白和吐溫20 防腐劑: < 0.1%* 0.01克 /升的溴酚藍鈉鹽 | 2×50毫升 2×50毫升 2×50毫升 |
終止液 1.2N 氫氧化鈉 | 15毫升 15毫升 15毫升 |
底物(立即可用) 對硝基苯磷酸鹽,不含其它溶劑的緩沖液 防腐劑:< 0.1% * (未開封瓶子中的底物可能會輕微變黃,但不會影響其質量) | 13毫升 13毫升 13毫升 |
帶有標準曲線和評估表的質量控制文件 (抗體以IU/毫升或U/毫升計量) | 1 1 1 |
ELISA classic百日咳 IgG:
| 平均消光值 (OD) | 檢測內(nèi)分析(CV%) | 平均消光值 (OD) | 檢測間分析(CV%) |
樣品 陰性 弱陽性 強陽性 |
0.252 0.512 1.742 |
13.71 9.61 4.50 |
- - - |
- - - |
陰性 陰性 弱陽性 | - - - | - - - | 0.264 0.360 0.613 | 8.51 11.72 16.50 |
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細菌能降解多種有機化合物的能力也常被用來清除污染,稱做生物復育(bioremediation )。舉例來說,科學家利用嗜甲烷菌(methanotroph)來分解美國佐治亞州的三氯乙烯和四氯乙烯污染。
細菌也是生物學的重要實驗材料,科學家曾用肺炎球菌發(fā)現(xiàn)并證實DNA是遺傳物質。隨著遺傳工程的興起,科學家已獲得了能產(chǎn)生胰島素等的大腸桿菌和對烴類有很強分解能力的假單胞菌的新菌株。
乙肝二對半檢查中, 如果乙肝表面抗原(HBsAg)、乙肝e抗原(HBeAg)和乙肝核心抗體(HBcAb)同時出現(xiàn)陽性,通常被稱為大三陽。提示乙型肝炎病毒在體內(nèi)復制(繁殖)活躍,且傳染性較強。這個所謂傳染性較強,是指通過血液傳播的能力較強,并不是說日常生活接觸可以傳播。所以與大三陽的患者(嚴格來講,不能都算是患者,因為乙肝攜帶者不算是真正意義上的病人) 親密接觸無須過分擔心緊張,尤其是男女朋友之間,不應該為此而斷絕關系,這是沒有必要的。但大三陽也有兩種情況,一種是大三陽攜帶,一種是大三陽肝炎。一種是肝功正常的患者,所以只是攜帶者而已。說明這些患者雖然病毒在體內(nèi)較活躍,但并沒有引起嚴重的肝損害。這些患者可以正常工作和學習。不需任何治療,任何關于護肝、抗病毒的治療都不需要,但是應該定期檢查肝功能,一旦出現(xiàn)異常,就要考慮是否該進行抗病毒治療了。不要喝酒。
大三陽攜帶者,如果仔細分的話,可以分為兩種。
(1)其一:自然攜帶,也就是說,嬰幼兒期感染乙肝病毒,稱為攜帶狀態(tài),以后一直都是攜帶狀態(tài),沒有轉變成肝炎。直至幾十年后攜帶狀態(tài)可能慢慢消失,病毒*被機體清楚,攜帶者變成正常人。乙肝感染不是終身感染。
(2)其二:非活動性攜帶,也就是說,患者本來是乙型肝炎狀態(tài)(記住,是肝炎,不是攜帶),肝功能異常,病毒含量較高,但經(jīng)過干擾素或核苷類藥抗病毒治療后取得療效,病毒含量水平下降,肝功能恢復正常,這時候看起來跟自然攜帶差不多,都是表面抗原陽性,肝功能正常,病毒含量低(檢測不出),但它跟自然攜帶又確實不大一樣。
The ability of bacteria to degrade many organic compounds is also often used to remove contamination, called bioremediation. For example, scientists use methanotrophs to decompose trichlorethylene and tetrachlorethylene in Georgia, the United States.
Bacteria are also important biological materials for experiments. Scientists have discovered with pneumococci and confirmed that DNA is a genetic material. With the advent of genetic engineering, scientists have acquired new strains of Pseudomonas that produce E. coli, such as insulin, and have a strong ability to decompose hydrocarbons.
In hepatitis B two-and-a-half check-ups, hepatitis B surface antigen (HBsAg), hepatitis B e antigen (HBeAg) and hepatitis B core antibody (HBcAb) are often positive at the same time. Tip Hepatitis B virus in vivo replication (reproduction) active, and highly contagious. The so-called highly contagious, refers to the strong ability to spread through the blood, does not mean that exposure to daily life can spread. Therefore, with Sanyang patients (strictly speaking, can not be regarded as patients, because hepatitis B carriers are not real sense of the patient) intimate contact without undue worry about tension, especially between men and women, should not be cut off for this reason Relationships, this is not necessary. However, there are two big Sanyang situation, one is carried Sanyanganyang, one is Big Three positive hepatitis. One is normal liver function patients, it is only carriers. Although these patients in the virus more active in the body, but did not cause serious liver damage. These patients can work and study properly. Without any treatment, any treatment of liver and antivirus is not needed, but liver function should be checked regularly. Once abnormal, it is necessary to consider whether antiviral therapy should be taken. do not drink alcohol.
Big Sanyang carriers, if carefully divided, can be divided into two kinds.
(1) First, it is naturally carried, that is to say, infants and young children are infected with hepatitis B virus and are called "carrying status". Since then they have always been carrying status and have not converted to hepatitis. Until decades later, the state of the carrier may slowly disappear, and the virus is compley cleared by the body and the carrier becomes a normal person. Hepatitis B infection is not a lifelong infection.
(2) The second: inactive carrying, that is, the patient was originally hepatitis B status (remember, is hepatitis, not to carry), abnormal liver function, high viral content, but after interferon or nucleosides Therapeutic efficacy after drug anti-virus treatment, viral levels decreased, liver function returned to normal, this time seems to carry almost the same nature, are surface antigen-positive, normal liver function, low virus content (undetectable), but it with nature Carry really is not the same.
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