甲型流感病毒核酸熒光探針法檢測試劑盒

廣州健侖生物科技有限公司

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甲型流感病毒核酸熒光探針法檢測試劑盒

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【Product Name】 Common Name: H7N9qin influenza virus nucleic acid detection kit (PCR-fluorescence probe method)
English name: Detection Kit for Avian Influenza Virus Subtype H7N9 RNA (PCR-Fluorescence Probing)
【Packing】 large package, 48 reaction / box
【expected usage】
Influenza virus can be divided into A (A), B (B), C (C) three types. Among them, influenza A virus can be divided into 1-16 subtypes according to the influenza virus hemagglutinin protein (HA), according to the virus neuraminidase protein (NA) can be divided into 1-9 subtypes, HA Different subtypes can be combined with different subtypes of NA to form different influenza viruses. The qin class, especially water qin, is the natural host for all of these flu viruses and the H7N9 qin flu virus is one of them. H7N9 influenza virus can infect both qin class, also can infect people. People infected with H7N9qin flu will develop severe pneumonia, symptoms include fever, cough, difficulty breathing and so on.
This kit is suitable for the detection of H7N9qin influenza virus RNA in samples of respiratory tract, serum, lung tissue, etc. It can be used for laboratory diagnosis and host animal monitoring of the virus infection. Test results for research purposes only, not for clinical diagnosis.
【Test principle】
This kit is based on real-time fluorescence PCR technology, respectively, selected H7N9qin influenza virus HA gene and NA gene conserved region as the amplification target region, design specific primer probe, by one-step real-time PCR amplification of H7N9qin influenza virus qualitative Detection. In addition to the specific primers and specific fluorescent probes in the reaction system, corresponding PCR reaction Buffer, reverse transcriptase, Hot-Start Taq enzyme, dNTPs, Mg2 + and other components can be achieved The H7N9qin influenza virus nucleic acid is sensitively and specifically detected.
【Main components】

Sample Requirements

1 Applicable sample types: respiratory samples, serum, lung tissue.

2 sample collection, storage and delivery.

Specimen collection, storage, delivery, please follow the "human infection H7N9 bird flu virus specimen collection and laboratory testing strategy."

It is recommended to take 200μl of liquid sample for nucleic acid extraction; tissue samples can be extracted with a grinding fluid. Zhongshan University Daan Gene Co., Ltd. can produce nucleic acid extraction kit, please follow the kit instructions; also choose other suitable commercial products. The internal standard solution in this kit is involved in the extraction process. If no internal standard is specified in the extraction kit, 4 μl of internal standard solution can be added to each 200 μl sample for nucleic acid extraction.

Negative controls in this kit are involved in the extraction and are used to monitor the environment. AIV H7N9 positive controls are not involved in the extraction and are used to control the quality of PCR reagents.

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【公司名稱】 廣州健侖生物科技有限公司
【市場部】    楊永漢

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【騰訊  】 2042552662
【公司地址】 廣州清華科技園創(chuàng)新基地番禺石樓鎮(zhèn)創(chuàng)啟路63號二期2幢101-103室