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甲型流感導致腦膜炎核酸檢測試劑盒

廣州健侖生物科技有限公司

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甲型流感導致腦膜炎核酸檢測試劑盒

【產品名稱】通用名稱：H7N9qin流感病毒核酸檢測試劑盒（PCR-熒光探針法）
英文名稱：Detection Kit for Avian Influenza Virus Subtype H7N9 RNA (PCR-Fluorescence Probing)  
【包裝規(guī)格】大包裝，48反應/盒
【預期用途】
流感病毒可分為甲（Ａ）、乙（Ｂ）、丙（Ｃ）三型。其中，甲型流感依據流感病毒血凝素蛋白（HA）的不同可分為1-16種亞型，根據病毒神經氨酸酶蛋白（NA）的不同可分為1-9種亞型，HA不同亞型可以與NA的不同亞型相互組合形成不同的流感病毒。而qin類特別是水qin是所有這些流感病毒的自然宿主，H7N9qin流感病毒是其中的一種。H7N9流感病毒既可以感染qin類，也可以感染人。人感染H7N9qin流感會出現嚴重肺炎，癥狀包括發(fā)燒、咳嗽、呼吸困難等。
本試劑盒適用于檢測呼吸道樣本、血清、肺組織等樣本中H7N9qin流感病毒RNA，可用于該病毒感染的實驗室診斷和宿主動物的監(jiān)控。檢測結果僅供研究，不用于臨床診斷。
【檢驗原理】
本試劑盒基于實時熒光PCR技術，分別選取H7N9qin流感病毒的HA基因和NA基因保守區(qū)作為擴增靶區(qū)域，設計特異性引物探針，通過一步法實時熒光PCR體系擴增對H7N9qin流感病毒進行定性檢測。反應體系中除特異性引物和特異性熒光探針外，還配以對應的PCR反應Buffer、逆轉錄酶、Hot-Start Taq酶、核苷酸單體（dNTPs）、Mg2+等成分，可實現對H7N9qin流感病毒核酸靈敏特異地檢測。

【Product Name】 Common Name: H7N9qin influenza virus nucleic acid detection kit (PCR-fluorescence probe method)
English name: Detection Kit for Avian Influenza Virus Subtype H7N9 RNA (PCR-Fluorescence Probing)
【Packing】 large package, 48 reaction / box
【expected usage】
Influenza virus can be divided into A (A), B (B), C (C) three types. Among them, influenza A virus can be divided into 1-16 subtypes according to the influenza virus hemagglutinin protein (HA), according to the virus neuraminidase protein (NA) can be divided into 1-9 subtypes, HA Different subtypes can be combined with different subtypes of NA to form different influenza viruses. The qin class, especially water qin, is the natural host for all of these flu viruses and the H7N9 qin flu virus is one of them. H7N9 influenza virus can infect both qin class, also can infect people. People infected with H7N9qin flu will develop severe pneumonia, symptoms include fever, cough, difficulty breathing and so on.
This kit is suitable for the detection of H7N9qin influenza virus RNA in samples of respiratory tract, serum, lung tissue, etc. It can be used for laboratory diagnosis and host animal monitoring of the virus infection. Test results for research purposes only, not for clinical diagnosis.
【Test principle】
This kit is based on real-time fluorescence PCR technology, respectively, selected H7N9qin influenza virus HA gene and NA gene conserved region as the amplification target region, design specific primer probe, by one-step real-time PCR amplification of H7N9qin influenza virus qualitative Detection. In addition to the specific primers and specific fluorescent probes in the reaction system, corresponding PCR reaction Buffer, reverse transcriptase, Hot-Start Taq enzyme, dNTPs, Mg2 + and other components can be achieved The H7N9qin influenza virus nucleic acid is sensitively and specifically detected.
【Main components】

組分名稱		規(guī)格	數量
PCR檢測試劑	AIV H7 PCR反應液A	816µl/管	1
	AIV N9 PCR反應液A	816µl/管	1
	AIV H7N9 PCR反應液B	288µl/管	1
	AIV H7N9內標溶液	240µl/管	1
質控品	陰性質控品	200µl/管	1
	AIV H7N9陽性質控品	200µl/管	1

Sample Requirements

1 Applicable sample types: respiratory samples, serum, lung tissue.

2 sample collection, storage and delivery.

Specimen collection, storage, delivery, please follow the "human infection H7N9 bird flu virus specimen collection and laboratory testing strategy."

It is recommended to take 200μl of liquid sample for nucleic acid extraction; tissue samples can be extracted with a grinding fluid. Zhongshan University Daan Gene Co., Ltd. can produce nucleic acid extraction kit, please follow the kit instructions; also choose other suitable commercial products. The internal standard solution in this kit is involved in the extraction process. If no internal standard is specified in the extraction kit, 4 μl of internal standard solution can be added to each 200 μl sample for nucleic acid extraction.

Negative controls in this kit are involved in the extraction and are used to monitor the environment. AIV H7N9 positive controls are not involved in the extraction and are used to control the quality of PCR reagents.

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【公司名稱】 廣州健侖生物科技有限公司
【市場部】    楊永漢

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【騰訊  】 2042552662
【公司地址】 廣州清華科技園創(chuàng)新基地番禺石樓鎮(zhèn)創(chuàng)啟路63號二期2幢101-103室

濕疹型藥疹一般具有以下幾個特點：①濕疹型藥疹為后天獲得，雖然和過敏素質與遺傳有密切關系，但藥物過敏不能直接由親體遺傳；②濕疹型藥疹一般均發(fā)生于多次藥物接觸后，而絕少發(fā)生于*接觸者；③濕疹型藥疹的*發(fā)病均有潛伏期，一般至少經1周左右，而再次發(fā)病則無潛伏期，可以即刻發(fā)??；④濕疹型藥疹的激發(fā)劑量一般均較低，患者一旦致敏，則往往可由極少量的藥物接觸麗致發(fā)作；⑤濕疹型藥疹一般具有典型的癥狀或體征；⑥濕疹型藥疹患者僅見于少數用藥患者。在早期或急性階段，患處有成片的紅斑，密集或疏散的小丘疹，或是肉眼難見的水皰，嚴重時有大片滲液及糜爛；在亞急性狀態(tài)，滲液減少及結痂，患處由鮮紅變暗紅，沒有大片的糜爛；在慢性狀態(tài)，滲液更少或*干燥而結痂，往往和鱗屑混合而成鱗屑痂，患處顏色更暗或是發(fā)十色素沉著，有時色素減少，在皮紋尤其運動程度較大的部位容易發(fā)生裂口，長期摩擦搔抓能引起顯著的苔蘚樣化，和神經性皮炎(慢性單純苔蘚)不易區(qū)別。 濕疹常有多種形態(tài)，容易減輕、加重或復發(fā)，邊界一般不太清楚。皮疹容易發(fā)生于兩側并或多或少的對稱，根據急性或慢性程度而有紅斑、丘疹、水皰、糜爛、鱗屑、痂、色素增加或減少、皸裂或苔蘚樣化等不同的表現，其中數種表現往往混雜在一起，有時先后發(fā)生。如有繼發(fā)性感染，還可有膿皰等皮損。

慢性濕疹往往是由急性濕疹經過亞急性階段轉變而成，但這種變化過程沒有明顯的界線，可同時存在著急性、亞急性及慢性的表現。有些病人的初起皮疹已經是慢性濕疹。

急性濕疹主要表現為紅斑、散布或成群的紅色丘疹、肉眼難見的水皰，有繼發(fā)感染時可起膿皰，嚴重時滲液較多，露出紅潤潮濕的糜爛面。

皮損的部位不定，可為局限性，也可彌漫散布于全身各處，在不同部位可有不同的表現，例如，頭皮濕疹常因化膿性感染而有膿皰瘡樣厚痂，而部濕疹往往是成片紅斑或分散的水皰丘疹，成年男人的胡須處濕疹可像須瘡，軀干濕疹常是紅斑鱗屑性；乳房濕疹zui常見于婦女尤其喂乳母親，奶頭容易皸裂而疼痛；手掌及足底的慢性濕疹因角化過度而像胼胝，皮紋處容易裂開而成皸裂性濕疹；發(fā)生于肘窩及腘窩的肢體濕疹常是慢性濕疹，而發(fā)生于小腿的墜積性濕疹常是靜脈曲張綜合征的一種表現。

Eczema type drug eruption generally has the following characteristics: ① eczema type drug eruption acquired for the day after tomorrow, although the allergenic quality and genetic are closely related, but the drug allergy can not be directly inherited by the parent; ② eczema drug eruption usually occurred after multiple drug contact , And seldom occurred in the first contact; ③ the first onset of eczema-type drug eruption has a latent period, usually at least 1 week, while the incidence of the disease has no incubation period, the disease can be immediay; ④ eczema-type drug eruption dose are generally lower , Once the patient sensitized, you can often be caused by a very small amount of drug exposure caused by Polyglot; ⑤ eczema type drug eruption usually has the typical symptoms or signs; ⑥ eczema type drug eruption patients found only in a small number of patients. In the early or acute stage, the affected area has a patch of erythema, dense or scattered papules, or the unseen blisters, severe exudate and erosion; in the subacute state, exudate and scab, the affected area Dark red dark red, no large pieces of erosion; in chronic conditions, exudate less or compley dry and scab, often mixed with scaly scabs, the lesion is darker or ten pigmentation, sometimes reduced pigment, In the dermatoglyphic areas especially prone to greater movement of the gap, long-term friction scratching can cause significant mossification, and neurodermatitis (chronic simple moss) is not easy to distinguish. Eczema often a variety of forms, easy to reduce, aggravate or relapse, the border is generally not clear. Rashes easily occur on both sides and more or less symmetrical, according to the degree of acute or chronic erythema, papules, blisters, erosion, scales, scabs, pigment increase or decrease, chapped or mossy and other different performance, the number of The performance is often mixed together, sometimes in succession. If secondary infection, there may be pustular lesions.

Chronic eczema often results from a subacute phase of acute eczema. However, there is no clear boundary for this change, and acute, subacute, and chronic manifestations can occur at the same time. The initial rash in some patients is already chronic eczema.

Acute eczema mainly for the erythema, scattered or groups of red papules, the eye blister visible, secondary infection can be pustular, exudate in severe cases, exposing the rosy moist erosion surface.

Lesions of the site of indefinite, can be limited, but also diffuse throughout the body can spread in different parts may have different manifestations, for example, often due to suppurative scalp eczema impetious thick crust, and Department of eczema Often is a piece of erythema or scattered blisters pimples, adult men's beard eczema can be like a sore, trunk eczema often erythema scales; breast eczema is most common in women, especially breastfeeding mothers, nipples easily chapped and pain; palms and feet The bottom of the chronic eczema due to hyperkeratosis and the corpus callosum, dermatoglyphics easily rupture and chapped eczema; occurred in the elbow fossa and popliteal limb eczema is often chronic eczema, and occurs in the calf hypostatic eczema often A manifestation of varicose syndrome.