芬蘭 流感病毒抗原檢測(cè)試劑盒（膠體金法）

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芬蘭 流感病毒抗原檢測(cè)試劑盒（膠體金法）

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【公司名稱】 廣州健侖生物科技有限公司
【市場(chǎng)部】    楊永漢

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【騰訊  】 2042552662
【公司地址】 廣州清華科技園創(chuàng)新基地番禺石樓鎮(zhèn)創(chuàng)啟路63號(hào)二期2幢101-103室

一，免疫組織化學(xué)簡(jiǎn)介
免疫組織化學(xué)又稱免疫細(xì)胞化學(xué)，是指帶顯色劑標(biāo)記的特異性抗體在組織細(xì)胞原位通過(guò)抗原抗體反應(yīng)和組織化學(xué)的呈色反應(yīng)，對(duì)相應(yīng)炕原進(jìn)行定性、定位、定量測(cè)定的一項(xiàng)新技術(shù)。它把免疫反應(yīng)的特異性、組織化學(xué)的可見(jiàn)性巧妙地結(jié)合起來(lái)，借助顯微鏡(包括熒光顯微鏡、電子顯微鏡)的顯像和放大作用，在細(xì)胞、亞細(xì)胞水平檢測(cè)各種抗原物質(zhì)(如蛋白質(zhì)、多肽、酶、激素、病原體以及受體等)。
二，免疫組化技術(shù)的基本原理
免疫組化技術(shù)是一種綜合定性、定位和定量；形態(tài)、機(jī)能和代謝密切結(jié)合為一體的研究和檢測(cè)技術(shù)。在原位檢測(cè)出病原的同時(shí)，還能觀察到組織病變與該病原的關(guān)系，確認(rèn)受染細(xì)胞類(lèi)型，從而有助于了解疾病的發(fā)病機(jī)理和病理過(guò)程。
免疫酶組化技術(shù)是通過(guò)共價(jià)鍵將酶連接在抗體上，制成酶標(biāo)抗體，再借酶對(duì)底物的特異催化作用，生成有色的不溶性產(chǎn)物或具有一定電子密度的顆粒，于普通顯微鏡或電鏡下進(jìn)行細(xì)胞表面及細(xì)胞內(nèi)各種抗原成分的定位，根據(jù)酶標(biāo)記的部位可將其分為直接法（一步法）、間接法（二步法）、橋聯(lián)法（多步法）等，用于標(biāo)記的抗體可以是用免疫動(dòng)物制備的多克隆抗體或特異性單克隆抗體，是特異性強(qiáng)的高效價(jià)的單克隆抗體。直接法是將酶直接標(biāo)記在*抗體上，間接法是將酶標(biāo)記在第二抗體上，檢測(cè)組織細(xì)胞內(nèi)的特定抗原物質(zhì)。目前通常選用免疫酶組化間接染色法。

First, the introduction of immunohistochemistry
Immunohistochemistry, also known as immunocytochemistry, refers to the specific reagent with a reagent labeled with a chromogenic reagent in situ through the antigen-antibody reaction and histochemical color reaction, the corresponding kang original qualitative, localization, quantitative determination of a New technology. It cleverly combines the specificity of immunological reactions and the visibility of histochemistry to detect the presence of various antigenic substances (such as proteins, proteins, and proteins) at the cellular and subcellular level through the imaging and amplification of microscopy (including fluorescence microscopy and electron microscopy) Polypeptides, enzymes, hormones, pathogens and receptors, etc.).
Second, the basic principles of immunohistochemistry
Immunohistochemistry is a comprehensive qualitative, positioning and quantitative; morphology, function and metabolism as one of the closely integrated research and testing technology. In situ detection of the pathogen at the same time, but also observed the relationship between tissue lesions and the pathogen to confirm the type of infected cells, which helps to understand the pathogenesis of the disease and pathological processes.
Enzyme immunohistochemistry is a covalent bond to the enzyme linked to the antibody, made of enzyme-labeled antibody, and then by the enzyme on the substrate for specific catalysis to generate colored insoluble products or have a certain electron density of particles in the ordinary microscope Or electron microscopy of cell surface and intracellular antigen components of the location, according to the site of enzyme markers can be divided into direct method (one-step method), indirect method (two-step method), bridge method (multi-step method) The antibody used for labeling may be a polyclonal antibody or a specific monoclonal antibody prepared by immunizing an animal, preferably a highly specific, highly titer monoclonal antibody. The direct method is to label the enzyme directly on the primary antibody, the indirect method is to label the enzyme on the secondary antibody and detect the specific antigenic substance in the tissue cells. At present, immunoenzymatic staining is often used indirect staining.