卡氏肺孢子蟲檢測試劑盒（PCR-熒光探針法）

廣州健侖生物科技有限公司

One tube multiplex for detection AND quantification of Pneumocystis jirovecii and internal control.
一個管多重檢測和定量的卡氏肺孢子蟲和內(nèi)部控制。
Principle
Multiplex real-time PCR for detection of pathogen genes by TaqMan® technology
Targets
One tube multiplex PCR:
Pneumocystis jirovecii
internal control
Specimen
This test is for use with extracted nucleic acid from respiratory samples (throat/nasal swabs, bronchoalveolar lavage and sputum) of human origin
Storage
-20°C
Shelf life
12 months from manufacture

卡氏肺孢子蟲檢測試劑盒（PCR-熒光探針法）

我司還提供其它進口或國產(chǎn)試劑盒：登革熱、瘧疾、流感、A鏈球菌、合胞病毒、腮病毒、乙腦、寨卡、黃熱病、基孔肯雅熱、克錐蟲病、違禁品濫用、肺炎球菌、軍團菌、化妝品檢測、食品安全檢測等試劑盒以及日本生研細菌分型診斷血清、德國SiFin診斷血清、丹麥SSI診斷血清等產(chǎn)品。

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【公司名稱】 廣州健侖生物科技有限公司
【市場部】    楊永漢

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【騰訊  】 2042552662
【公司地址】 廣州清華科技園創(chuàng)新基地番禺石樓鎮(zhèn)創(chuàng)啟路63號二期2幢101-103室

三、第二抗體的染色
12、加100 ul（2滴）雙染增強劑（試劑7），孵育；
13、用TPBS沖洗3 min×3次；
14、加100 ul（2滴）血清封閉溶液（試劑1）到切片上，室溫孵育10 min，倒掉（不能沖洗）；
15、加入稀釋好的一抗（按照說明推薦濃度和孵育條件）；
16、用TPBS沖洗5 min×3次；
17、加入100 ul（2滴）生物素化的二抗（試劑2)，室溫孵育10 min；
18、用TPBS沖洗5 min×3次；
19、加入100 ul（2滴）鏈霉親和素-HRP（試劑5）的二抗，室溫孵育10 min；
20、用TPBS沖洗5 min×3次；
21、 準備底物溶液：將1滴試劑6A和6B加入到1 ml去離子水中，充分混勻。再加入1滴試劑6C，充分混勻；
22、 加100 ul（2滴）配好的HRP底物溶液，室溫孵育（鏡下掌握孵育時間）；
23、 使用含有0.05%吐溫20的去離子水沖洗終止反應(yīng)；
24、 使用復(fù)染試劑染色（根據(jù)需要選擇）；
25、 封片劑（試劑8）封片，顯微鏡觀察并拍照。

Third, the second antibody staining
12, add 100 ul (2 drops) double staining enhancer (reagent 7), incubation;
13, rinsed with TPBS 3 min × 3 times;
14. Add 100 ul (2 drops) serum blocking solution (Reagent 1) to the sections and incubate at room temperature for 10 min.
15, add a good dilution of the first antibody (according to the instructions recommended concentration and incubation conditions);
16, rinsed with TPBS 5 min × 3 times;
17. Add 100 ul (2 drops) biotinylated secondary antibody (Reagent 2) and incubate for 10 min at room temperature.
18, rinsed with TPBS 5 min × 3 times;
19. Add 100 ul (2 drops) streptavidin-HRP (Reagent 5) secondary antibody and incubate for 10 min at room temperature.
20, rinsed with TPBS 5 min × 3 times;
21. Prepare Substrate Solution: Add 1 drop of Reagents 6A and 6B to 1 ml of deionized water and mix well. Then add 1 drop of reagent 6C, mix well;
22, add 100 ul (2 drops) with a good solution of HRP substrate, incubated at room temperature (microscopy grasp the incubation time);
23. Stop the reaction by rinsing with deionized water containing 0.05% Tween 20;
24, using stained reagents staining (according to need to choose);
25, sealing agent (reagent 8) sealing, microscopic observation and photographing.