PCR核酸試劑 糞便寄生蟲多重檢測(cè)PCR熒光試劑盒

廣州健侖生物科技有限公司

單管多重檢測(cè)溶組織內(nèi)阿米巴，隱孢子蟲屬，賈第鞭毛蟲和內(nèi)部對(duì)照。
One tube multiplex for detection of Entamoeba histolytica, Cryptosporidium spp., Giardia lamblia and internal control.

PCR核酸試劑 糞便寄生蟲多重檢測(cè)PCR熒光試劑盒

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【公司名稱】 廣州健侖生物科技有限公司
【市場(chǎng)部】    楊永漢

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【公司地址】 廣州清華科技園創(chuàng)新基地番禺石樓鎮(zhèn)創(chuàng)啟路63號(hào)二期2幢101-103室

（2）柱上吸附的過度標(biāo)記蛋白可繼續(xù)增加NaCl的濃度至 
2.0mol/L洗脫完。 
經(jīng)過DEAE-纖維素層析后的標(biāo)記抗體，其抗體量一般約損失50%，因此有些要求不太高的抗體，如抗細(xì)菌熒光抗體，不一定要這樣處理，可用染色效價(jià)測(cè)定的稀釋法除去非特異性染色。 
（五）熒光抗體稀釋法 
先測(cè)定熒光抗體特異性染色與非特異性染色的效價(jià)，若二者效價(jià)相差較大，則可將熒光抗體稀釋至一臨界濃度，使特異性染色呈陽性，而使非特異性染色保持陰性，稀釋方法和染色效價(jià)測(cè)定方法相同。 
（六）純化抗原法 
用各種方法提純單一成分的抗原是產(chǎn)生單價(jià)特異性抗體的zui主要條件。近代免疫化學(xué)技術(shù)（免疫吸收法）和柱層析法等提供了很大的可能性，可參考有關(guān)專著。 
（七）純化抗體法---免疫吸收法 
例如抗IgA血清的純化方法---免疫吸收法。如分泌型IgA（ SIgA）抗原純度不高，所制的抗血清常與IgG呈交叉反應(yīng)，為此需要吸收，常采用純化的人IgG戊二醛聚合物加以吸收純化。

(2) Over-labeled protein adsorbed on the column can continue to increase the concentration of NaCl to
2.0mol / L eluting finished.
After DEAE-cellulose chromatography of the labeled antibody, the amount of antibody is generally about 50% loss, so some less demanding antibodies, such as anti-bacterial fluorescent antibodies, do not have to deal with this method can be used to determine the dilution of the dye titer Non-specific staining is removed.
(E) Fluorescent antibody dilution method
First determine the fluorescent antibody-specific staining and non-specific staining titer, if the difference between the two titer, you can dilute the fluorescent antibody to a critical concentration, so that specific staining was positive, leaving non-specific staining remained negative, The same dilution method and determination of the titer.
(Vi) Purified antigen method
Purification of a single component of antigen by various means is the most important condition for the production of monovalent specific antibodies. Modern immunochemical techniques (immunosuppression) and column chromatography provides a great possibility, you can refer to the monograph.
(G) Purified antibody method --- immunosorbent assay
For example, anti-IgA serum purification method --- immune absorption method. Such as secreted IgA (SIgA) antigen purity is not high, the resulting antisera often cross-react with IgG, which needs to be absorbed, often using purified human IgG glutaraldehyde polymer to be purified by absorption.