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【文獻標題】 microRNA-328 in exosomes derived from M2 macrophages exerts a promotive effect on the progression of pulmonary fibrosis via FAM13A in a rat model

【作者】Meng-Ying Yao1, Wei-Hong Zhang2, Wen-Tao Ma1,et.al

【作者單位】鄭州大學

【文獻中引用產(chǎn)品】

生物素標記羊抗兔二抗

【DOI】 doi.org/10.1038/s12276-019-0255-x

【影響因子(IF)】 5.14

【出版期刊】 《EXPERIMENTAL AND MOLECULAR MEDICINE》

【產(chǎn)品原文引用】

Immunohistochemistry (IHC)

IHC was performed on 4% buffered formalin-fixed and paraffin-embedded sections (4 μm thick). Next, the sections were incubated with 100 μL of primary antibodies,including rabbit-anti α-SMA (1:500, ab108424, Abcam,Cambridge, MA, USA) and anti-Collagen I (5 μg/mL,ab34710, Abcam, Cambridge, MA, USA) antibodies.Then, the sections were incubated with a biotinylated goat anti-rabbit secondary antibody (1:100, HY90046, Shanghai Hengyuan Biotechnology Co., Ltd., Shanghai, China).

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