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首先讓我們熱烈恭賀中國農(nóng)業(yè)科學(xué)院的老師成功發(fā)表文獻。老師之前訂購了恒遠生物高敏ELISA試劑盒，使用了我們的產(chǎn)品在《Science of the Total Environment》雜志上發(fā)表了文獻——Assessing the bioavailability and biotoxicity of spiromesifen and its main metabolite spiromesifen-enol (M01) reveals the defense mechanisms of earthworms (Eisenia fetida) IF影響因子高達10.75分！恒遠生物在此也非常感謝老師對我們信任與支持，希望通過我們的不懈努力能為科研事業(yè)做出更大的貢獻！

這篇文獻非常值得研讀，其中的很多實驗方法和思路值得我們?nèi)W(xué)習(xí)，一篇文獻的誕生要費很多精力和心血，老師在做實驗的過程中也遇到了很多難題，我們的技術(shù)人員也盡力提供幫助，互相學(xué)習(xí)互相進步。小編想要把這篇文獻分享給大家，希望能夠?qū)ψ隹蒲械男』锇閭冇幸稽c點幫助，也希望大家積極的參與我們的“寫文獻贏獎學(xué)金"活動，我等著你們的投稿哦，下一個領(lǐng)獎學(xué)金的說不定就是你！

【文獻標題】Assessing the bioavailability and biotoxicity of spiromesifen and its main metabolite spiromesifen-enol (M01) reveals the defense mechanisms of earthworms (Eisenia fetida)

【作者】Jianwei Fang , Binning Wang , Kuan Fang ,et.a

【作者單位】中國農(nóng)業(yè)科學(xué)院（ Chinese Academy of Agricultural Sciences）

【文獻中引用產(chǎn)品】

蚯蚓超氧化物歧化酶（SOD）ELISA試劑盒活性

蚯蚓過氧化氫酶（CAT）ELISA試劑盒活性

蚯蚓丙二醛（MDA）ELISA試劑盒

【關(guān)鍵詞】UPLC-MS/MS,Transcriptome sequencing,SEM,TEM,Common DEGs

【DOI】doi.org/10.1016/j.scitotenv.2021.151910

【影響因子(IF)】10.75

【出版期刊】《Science of the Total Environment》

【產(chǎn)品原文引用】

2.6. Determination of oxidative and immune stress effects

The protein content was determined according to the manufacturer's instructions using a protein assay kit (Solarbio; China). The reaction between Coomassie brilliant blue and protein was monitored at 595 nm, and the protein content was determined based on the change in absorbance. The ·OH? content, SOD activity, CAT activity, and MDA content were measured using an ELISA kit (Shanghai Hengyuan Biotechnology Co., Ltd.; China). The contents or activities were determined according to the changes in the absorbance induced by the color reaction.

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