Invitrogen 15596-018 TRlzol Reagent* 介紹:
產品名稱:TRlzol Reagent;TRlzol;TRlzol試劑;TRlzol抽提試劑盒
貨 號:15596-018
規(guī) 格:200ml
品 牌:Invitrogen
國產/進口:進 口
儲存溫度:-20°C
特惠價格:致 電
庫存狀態(tài):現 貨
銷售商:上?;鄯f生物科技有限公司
慧穎生物專業(yè)供應Invitrogen 15596-018 TRlzol Reagent* ,常備現貨,*。主營Life Invitrogen產品,囊括TRlzol ?Reagent、TRIZOL LS Reagent 、B-27添加劑、凝膠填料、常規(guī)化學試劑、培養(yǎng)基及原料試劑、各類胎牛血清、馬血清等,如有需要,詳詢。
Invitrogen 15596-018 TRlzol Reagent* Description:
TRIzol? Reagent is a ready-to-use reagent, designed to isolate high quality total RNA (as well as DNA and proteins) from cell and tissue samples of human, animal, plant, yeast, or bacterial origin, within one hour. TRIzol? Reagent is a monophasic solution of phenol, guanidine isothiocyanate, and other proprietary components which facilitate the isolation of a variety of RNA species of large or small molecular size. TRIzol? Reagent maintains the integrity of the RNA due to highly effective inhibition of RNase activity while disrupting cells and dissolving cell components during sample homogenization. TRIzol? Reagent allows for simultaneous processing of a large number of samples, and is an improvement to the single-step RNA isolation method developed by Chomcynski and Sacchi (Chomczynski & Sacchi, 1987). TRIzol? Reagent allows the user to perform sequential precipitation of RNA, DNA, and proteins from a single sample (Chomczynski, 1993). After homogenizing the sample with TRIzol? Reagent, chloroform is added, and the homogenate is allowed to separate into a clear upper aqueous layer (containing RNA), an interphase, and a red lower organic layer (containing the DNA and proteins). RNA is precipitated from the aqueous layer with isopropanol. DNA is precipitated from the interphase/organic layer with ethanol. Protein is precipitated from the phenol-ethanol supernatant by isopropanol precipitation. The precipitated RNA, DNA, or protein is washed to remove impurities, and then resuspended for use in downstream applications.
.Isolated RNA can be used in RT-PCR, Northern Blot analysis, Dot Blot hybridization, poly(A)+ selection, in vitro translation, RNase protection assay, and molecular cloning.
. Isolated DNA can be used in PCR, Restriction Enzyme digestion, and Southern Blots.
. Isolated protein can be used for Western Blots, recovery of some enzymatic activity, and some immunoprecipitation.
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,慧穎現貨,確保正品來源。Invitrogen產品種類較多,本展臺無法一一體現,以上是部分產品,若有需要的產品,請及時與我們取得,咨詢。
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