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※ 使用C18和“苯基"雜和鍵合，擴展色譜柱的選擇性分離
※ PH穩(wěn)定性好 1—10之間穩(wěn)定使用
※ 多步高密度鍵合技術，保證樣品出峰尖銳對稱
※ 低流失，滿足液相質譜LC-MS使用要求
產品簡介
詳細介紹
Sphinx 擴展您檢測的選擇性
※ 使用C18和“苯基”雜和鍵合，擴展色譜柱的選擇性分離
※ PH穩(wěn)定性好 1—10之間穩(wěn)定使用
※ 多步高密度鍵合技術，保證樣品出峰尖銳對稱
※ 低流失，滿足液相質譜LC-MS使用要求
為避免常規(guī)高密度鍵合C18色譜柱的疏水性過強對極性化合物分析能力差的問題，Sphinx選擇性鍵入“苯基”官能團，增加了和帶雙鍵樣品間的∏-∏作用，具有特殊的選擇性能，所以sphinx特別適合分析帶“苯環(huán)”和“雙鍵”的樣品。
NUCLEODUR® Sphinx RP |
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High purity silica phase for HPLC | |
Bifunctional RP phase, balanced ratio of propylphenyl and C18 ligands | |
Properties |
| Key features:
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| Technical characteristics: | |
| Recommended application: |
產品應用:
下圖表明Sphinx和常規(guī)C8/C18色譜柱在鍵合方法和選擇性上的區(qū)別
流動相：甲醇：水=60:40流 速：1.0毫升/分鐘
進樣量：3 ul溫 度：30℃檢測器：UV 270nm
樣 品：1.兒茶素 2.蘆丁 R1=R3=DH,R2=O-rutinose 3.漆黃素 R1=R3=OH,R2=H 4.槲皮素 R1=R2=R3=OH 5.shannai酚R1=H,R2=R3=OH 6.異鼠李素 R1=OCH3，R2=R3=OH
訂貨信息:
EC standard columns for analytical HPLC |
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Ordering information | |
Eluent in column acetonitrile – water |
Length -> | 30 mm | 50 mm | 75 mm | 100 mm | 125 mm | 150 mm | 250 mm |
NUCLEODUR® Sphinx RP, 1.8 µm | |||||||
2 mm ID | 760821.20 | 760822.20 | 760825.20 | 760823.20 |
| 760824.20 |
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3 mm ID | 760821.30 | 760822.30 |
| 760823.30 |
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4 mm ID | 760821.40 | 760822.40 |
| 760823.40 |
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4.6 mm ID | 760821.46 | 760822.46 |
| 760823.46 |
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EC guard columns*: | 4 x 2 mm | 761920.20 |
| 4 x 3 mm | 761920.30 |
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NUCLEODUR® Sphinx RP, 3 µm | |||||||
2 mm ID |
| 760806.20 |
| 760812.20 | 760807.20 | 760805.20 | 760808.20 |
3 mm ID |
| 760806.30 |
| 760812.30 | 760807.30 | 760805.30 | 760808.30 |
4 mm ID |
| 760806.40 |
| 760812.40 | 760807.40 | 760805.40 | 760808.40 |
4.6 mm ID |
| 760806.46 | 760813.46 | 760812.46 | 760807.46 | 760805.46 | 760808.46 |
EC guard columns*: | 4 x 2 mm | 761921.20 |
| 4 x 3 mm | 761921.30 |
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CC guard columns**: | 8 x 3 mm | 761557.30 |
| 8 x 4 mm | 761557.40 |
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NUCLEODUR® Sphinx RP, 5 µm | |||||||
2 mm ID |
| 760800.20 |
| 760809.20 | 760801.20 | 760802.20 | 760803.20 |
3 mm ID |
| 760800.30 |
| 760809.30 | 760801.30 | 760802.30 | 760803.30 |
4 mm ID |
| 760800.40 |
| 760809.40 | 760801.40 | 760802.40 | 760803.40 |
4.6 mm ID |
| 760800.46 | 760815.46 | 760809.46 | 760801.46 | 760802.46 | 760803.46 |
EC guard columns*: | 4 x 2 mm | 761922.20 |
| 4 x 3 mm | 761922.30 |
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CC guard columns**: | 8 x 3 mm | 761550.30 |
| 8 x 4 mm | 761550.40 |
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Guard columns in packs of 3, EC columns in packs of 1 | |||||||
Guard columns for EC columns |
| EC columns with ID | Use guard | ||||
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| 2 mm | 3 mm | 4 mm | 4.6 mm | |
* | Column Protection System | EC guard column | 4/2 | 4/3 | 4/3 | 4/3 | REF 718966 |
** | ChromCart® guard column system | CC guard column | 8/3 | 8/3 | 8/4 | 8/4 | REF 721359 |
Custom-packed EC columns with other dimensions are available on request. | |||||||
top of page | |||||||
VarioPrep columns for preparative HPLC |
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Ordering information | |
Eluent in column acetonitrile – water |
Length | 10 mm ID | 21 mm ID | 32 mm ID | 40 mm ID |
NUCLEODUR® Sphinx RP, 5 µm | ||||
50 mm | 762372.100 | 762372.210 |
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125 mm | 762375.100 | 762375.210 |
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150 mm |
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| 762371.400 |
250 mm | 762373.100 | 762373.210 | 762373.320 | 762373.400 |
VP guard columns with NUCLEODUR® Sphinx RP | ||||
| 8 mm ID | 16 mm ID | 32 mm ID | |
10 mm* | 762390.80 | 762390.160 |
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15 mm* |
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| 762392.320 | |
* VP guard column | Size | Pack of | for VP column | required holder | REF |
VP 10/8 | 10 x 8 mm ID | 2 | 10 mm ID | 8 mm | 718251 |
VP 10/16 | 10 x 16 mm ID | 2 | 21 mm ID | 16 mm | 718256 |
VP 15/32 | 15 x 32 mm ID | 1 | 32–40 mm ID | 32 mm | 718253 |
Custom-packed VP columns with other dimensions are available on request. | |||||
For details of the VarioPrep column system click here. | |||||
Standard program* of columns packed with NUCLEODUR® Sphinx RP |
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Column system | Particle size: | 1.8 µm | 3 µm | 5 µm | |
Analytical | EC columns (standard system, 2–4.6 mm) |
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Preparative | VarioPrep columns (VP, ≥ 8 mm) |
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* Please note! Most combinations of MN column types, available column dimensions and particle sizes can be custom-packed on request. | |||||
