培安有限公司 |
010-65528800
高 (市場(chǎng)部)
- 電話:
- 010-65528800
- 手機(jī):
- 售后電話:
- 010-64278205
- 傳真:
- 010-65519722
- 聯(lián)系我時(shí),
- 告知來自化工儀器網(wǎng)
- 個(gè)性化:
- www.pynnco.net
- 商鋪網(wǎng)址:
- http://www.hbwxwy.cn/st26894/
- 公司網(wǎng)站:
- http://www.pynnco.com
利用壓力循環(huán)技術(shù)(PCT)從植物組織中提取RNA
閱讀:1280發(fā)布時(shí)間:2009-02-27
提 供 商: | 培安有限公司 | 資料大?。?/th> | 90.7686B |
---|---|---|---|
資料圖片: | 下載次數(shù): | 328次 | |
資料類型: | PDF 文件 | 瀏覽次數(shù): | 1280次 |
Extraction of nucleic acids from plants is a time consuming and labor intensive process. Two common methods of
extraction are (i) grinding individual samples with mortar and pestle, and (ii) mechanical mincing. Unfortunay,
these methods are prone to contamination, as the same tools are often reused sequentially for multiple
preparations. This risk of contamination is often unacceptable, especially in studies using rare or valuable
specimens, such as when breeding selection is critical (particularly with the advent of genetically
engineered crops), or when inlectual property discovery or protection is involved. Here we compare
cell isruption by Qiagen RNeasy with mortar and pestle to pressure cycling technology PCT) for the
extraction of RNA from corn sprouts, grape seeds, and grape skin.
利用壓力循環(huán)技術(shù)(PCT)從植物組織中提取RNA
從植物中提取核酸是一件非常耗時(shí)耗力的工作。目前常見的提取方法有人工研磨和機(jī)械破碎兩種方法。因?yàn)檠心スぞ吆推扑榈额^經(jīng)常反復(fù)使用,所以這兩種 方法非常容易造成污染。在對(duì)非常或者非常有價(jià)值的樣品進(jìn)行研究時(shí),污染常常不能被接受。本文介紹了利用壓力循環(huán)技術(shù)(PCT)和傳統(tǒng)研磨技術(shù)兩種不同 的方法從玉米芽、葡萄種子和葡萄皮中提取RNA,并對(duì)這兩種方法進(jìn)行了比較。
以上信息由企業(yè)自行提供,信息內(nèi)容的真實(shí)性、準(zhǔn)確性和合法性由相關(guān)企業(yè)負(fù)責(zé),化工儀器網(wǎng)對(duì)此不承擔(dān)任何保證責(zé)任。 溫馨提示:為規(guī)避購買風(fēng)險(xiǎn),建議您在購買產(chǎn)品前務(wù)必確認(rèn)供應(yīng)商資質(zhì)及產(chǎn)品質(zhì)量。