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insphero 3D細胞懸滴培養(yǎng)板構(gòu)建3D骨肉瘤模型
點擊次數(shù):3154 發(fā)布時間:2014-11-17
insphero公司聯(lián)合蘇黎世大學的骨科研究實驗室、化學和生物化學研究所,應(yīng)用insphero 3D細胞懸滴培養(yǎng)板,構(gòu)建3D骨肉瘤模型。
文章“An in vitro osteosarcoma 3D microtissue model for drug development”于2014年11月10號發(fā)表于 Journal of Biotechnology上
(鏈接地址:http://www.ncbi.nlm.nih.gov/pubmed/25234575)
作者介紹:
Markus Rimanna, 1, Sandra Laternsera, 1, Ana Gvozdenovicb, Roman Muffb, Bruno Fuchsb, Jens M. Kelmc, Ursula Graf-Hausnera, ,
a Institute of Chemistry and Biological Chemistry (ICBC), Zurich University of Applied Sciences, Waedenswil, Switzerland
b Laboratory for Orthopedic Research, Department of Orthopedics, University of Zurich, Switzerland
c InSphero AG, Schlieren, Switzerland
文章亮點:
1.Microtissues (3D) from three different osteosarcoma cell lines were developed.
取三種不同的骨肉瘤細胞系(SaOS-2、HOS和MG-63細胞),采用insphero 3D細胞懸滴培養(yǎng)板構(gòu)建3D骨肉瘤微組織
2. Microtissues from patient-derived osteosarcoma were produced.
骨肉瘤細胞系(SaOS-2、HOS和MG-63細胞),均取自成骨肉瘤和軟骨肉瘤病人
3.Inherent osteosarcoma tissue heterogeneity was shown with different microtissues.
不同的微組織,會顯示出骨肉瘤固有的組織異質(zhì)性
4.Five drug responses were analysed in 3D showing differences compared to 2D.
用五種藥物(阿霉素、順鉑、牛磺羅定、培美曲塞、紫杉醇)對3D骨肉瘤微組織用藥,實驗結(jié)果是不同于常規(guī)2D細胞培養(yǎng)的
總結(jié):
The 3D microtissues reflect the tissue heterogeneity of OS and are potential suitable tools for drug development towards personalised medicine
3D微組織能夠反映出骨肉瘤(OS)固有的組織異質(zhì)性,它將成為藥物開發(fā)以及走向個人化醫(yī)療過程中的有潛力工具。