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當(dāng)前位置:上海信裕生物科技有限公司>>技術(shù)文章>>Pim-3原癌基因(PIM3)重組蛋白
Pim-3原癌基因(PIM3)重組蛋白
Recombinant Pim-3 Oncogene (PIM3)
FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!
Organism species | Homo sapiens (Human) |
Product No. | XY637Hu01 |
Source | Prokaryotic expression |
Host | E.coli |
Purity | > 95% |
UOM | 50ug |
Predicted Molecular Mass | 39.6kDa |
Concentration | n/a |
Applications | SDS-PAGE; WB; ELISA; IP. |
Endotoxin Level | <1.0EU per 1µg (determined by the LAL method) |
Residues | Met1~Leu326 with two N-terminal Tags, His-tag and T7-tag |
Formulation | Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl. |
Pim-3原癌基因(PIM3)重組蛋白 SEQUENCES
The sequence of the target protein is listed below.
MLLSKFGSLA HLCGPGGVDH LPVKILQPAK ADKESFEKAY QVGAVLGSGG FGTVYAGSRI ADGLPVAVKH VVKERVTEWG SLGGATVPLE VVLLRKVGAA GGARGVIRLL DWFERPDGFL LVLERPEPAQ DLFDFITERG ALDEPLARRF FAQVLAAVRH CHSCGVVHRD IKDENLLVDL RSGELKLIDF GSGALLKDTV YTDFDGTRVY SPPEWIRYHR YHGRSATVWS LGVLLYDMVC GDIPFEQDEE ILRGRLLFRR RVSPECLI RWCLSLRPSE RPSLDQIAAH PWMLGADGGV PESCDLRLCT LDPDDVASTT SSSESL
Pim-3原癌基因(PIM3)重組蛋白 USAGE
Reconstitute in sterile PBS, pH7.2-pH7.4.
STORAGE AND STABILITY
Storage: Avoid repeated freeze/thaw cycles. Store at 2-8oC for one month. Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
Pim-3原癌基因(PIM3)重組蛋白 About the MARKER (complimentary)
Effective Size Range: 10kDa to 70kDa.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
Ready-to-use: No need to heat, dilute or add reducing agents before use.
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