ATP6V0D2 抗體 

抗原
ATPase, H+ Transporting, Lysosomal V0 Subunit D2 (ATP6V0D2)
別名 AI324824, V-ATPase, 1620401A02Rik
抗原表位
其他選擇 Isoform 1
Isoform 1(3)
適用
其他選擇 Arabidopsis thaliana, Chlamydomonas reinhardtii (C. reinhardtii), Barley (Hordeum vulgare), Tomato (Solanum lycopersicum), Lilium longiflorum (Trumpet lily), Rice (Oryza sativa), Maize/Corn (Zea mays), Pteris vittata (Chinese ladder brake)
Arabidopsis thaliana(6),Tomato (Solanum lycopersicum) (3),Nicotiana tabacum (2),Barley (Hordeum vulgare) (1),Chlamydomonas reinhardtii (C. reinhardtii) (1),Lilium longiflorum (Trumpet lily) (1),Maize/Corn (Zea mays) (1),Pinus sylvestris (1),Pteris vittata (Chinese ladder brake) (1),Rice (Oryza sativa) (1),Ricinus communis (1)
宿主
其他選擇 Rabbit
Rabbit(5),Goat (2)
克隆類型
多克隆
應(yīng)用范圍
其他選擇 Western Blotting (WB), Immunohistochemistry (IHC)
Western Blotting (WB)(7),ELISA (4),Immunohistochemistry (IHC) (1)
[Pubmed] 5 references available
規(guī)格 200 μL
發(fā)貨至 中國 (更改)
發(fā)貨時間 24至30個工作日

ATP6V0D2 抗體 

產(chǎn)品細節(jié)

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產(chǎn)品細節(jié)

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免疫原 KLH-conjugated synthetic peptide chosen from subunit E of plant V-ATPase including Arabidopsis thaliana At4g11150. Peptide is conserved in vacuolar H+-ATPase subunit E, isoform 1 to 3 (VHA-E1).
交叉反應(yīng) （詳細） No cross-reactivity with: mangrove plants,
產(chǎn)品特性 Expected / apparent Molecular Weight of the Antigene: 26 / 31 kDa (Arabidopsis thaliana)
純化方法 serum

目標詳細情況

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 目標詳細情況

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別名 V-ATPase
背景 AGI Code: At4g11150
Plant vacuole V-ATPase is responsible for energization of transport of ions and metabolites, and acts as well 'house-keeping' and as a stress response enzyme. V-ATPase is a multi-subunit enzyme composed of a membrane sector and a cytosolic catalytic sector. It is related to the FoF1 ATP synthase. Alternative protein names: Vacuolar proton pump subunit E, Protein EMBRYO DEFECTIVE 2448
分子量 expected: 26 kDa, apparent: 31 kDa (Arabidopsis thaliana)
UniProt Q39258

使用細節(jié)

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 使用細節(jié)

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應(yīng)用備注 Recommended Dilution: 1 : 2 000 - 1 : 5000 with alkaline phosphatase (WB), 1 : 50 (IHC).
Cellular [compartment marker] of tonoplast membrane
說明

V-ATPase is very sensitive for the redox of the SDS buffer. We recommend using at least 50-100 mM DTT freshly prepared before handling the sample.Immunostaining protocol using V-ATPase antibodies can be found here.

限制 For Research Use only

貯存及處理

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貯存及處理

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狀態(tài) Lyophilized
溶解方式 For reconstitution add 10 µL of sterile water.
注意事項 Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Once reconstituted make aliquots to avoid repreated freeze-thaw cycles.
儲存條件 -20 °C

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