Interleukin 4 (IL4) 抗體

抗原
Interleukin 4 (IL4)
別名 IL4, IL-4, BCGF-1, BCGF1, BSF-1, BSF1, Il-4, Il4e12, IL2
適用
其他選擇 Murine
Human(220),Murine (148),Rat ((Rattus)) (32),Bovine (11),Pig (Porcine) (7),Dog (Canine) (6),Rabbit (4),Goat (2),Horse (Equine) (2),Musid (2),Sheep (Ovine) (2),Rhesus Monkey (1),Wild boar (Sus scrofa) (1)
宿主
其他選擇 Rabbit
Rabbit(147),Rat (139),Mouse (111),Sheep (6),Goat (3),Guinea Pig (3)
克隆類型
多克隆
標記
其他選擇 非結(jié)合性
Biotin(52),PE (29),FITC (16),APC (12),HRP (8),Alexa Fluor 488 (4),Allophycocyanin (3),PerCP (3),Alexa Fluor 350 (2),Alexa Fluor 555 (2),Alexa Fluor 647 (2),Cy3 (2),Cy5 (2),Cy5.5 (2),Cy7 (2),Cy7,PE (2),DyLight 488 (2),DyLight 550 (2),DyLight 594 (2),DyLight 650 (2),DyLight 755 (2),PE,Cy7 (2),Alkaline Phosphatase (AP) (1)
應(yīng)用范圍
其他選擇 Enzyme Immunoassay (EIA), Functional Studies (Func), Western Blotting (WB)
ELISA(204),Western Blotting (WB) (200),Flow Cytometry (FACS) (126),Immunohistochemistry (IHC) (50),Neutralization (Neut) (35),Immunohistochemistry (Frozen Sections) (IHC (fro)) (29),Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (27),Immunoprecipitation (IP) (26),Enzyme Immunoassay (EIA) (23),Immunocytochemistry (ICC) (23),ELISpot (21),Immunofluorescence (Paraffin-embedded Sections) (IF (p)) (20),Functional Studies (Func) (19),Immunofluorescence (IF) (18),Blocking Antibody (Inhibition) (8),Intracellular Staining (ICS) (8),ELISA (Detection) (4),Immunoassay (IA) (4),Transfected cell culture (TCC) (4),Dot Blot (DB) (3),ELISA (Capture) (3),Blocking Reagent (BR) (1),ELISpot (Capture) (1),ELISpot (Detection) (1),Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (1),Immunohistochemistry (Frozen Paraffin-embedded Sections) (IHC (frpe)) (1),Radioimmunoassay (RIA) (1)
[Pubmed] 2 references available
規(guī)格 0.1 mg
發(fā)貨至 中國 (更改)
發(fā)貨時間 29至35個工作日

Interleukin 4 (IL4) 抗體

產(chǎn)品細節(jié)

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產(chǎn)品細節(jié)

顯示 隱藏
免疫原 Highly pure (>98%) recombinant mIL-4 (murine Interleukin-4).
特異性 Recognises Mouse Interleukin-4 (IL-4).
純化方法 Affinity chromatography.

目標詳細情況

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Interleukin 4 (IL4) 抗體目標詳細情況

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別名 Interleukin-4 / IL4
背景 IL4 is a pleiotropic cytokine produced by activated T cells, mast cells and basophils. It is a ligand for Interleukin 4 receptor. The Interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. IL4 elicits many different biological responses, but has two dominant functions. The first is regulating differentiation of naive CD4+ T cell to the Th2 type. Th2 cells produce IL4, IL5, IL10 and IL13, which tend to favor a humoral immune response while suppressing a cell mediated immune response controlled by Th1 cells. STAT6, a signal transducer and activator of transcription, has been shown to play a central role in mediating the immune regulatory signal of this cytokine. The second is regulating IgE and IgG1 production by B cells. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported.Synonyms: B-cell stimulatory factor 1, BSF1, Binetrakin, IL-4, Lymphocyte stimulatory factor 1, Pitrakinra
基因ID 16189
NCBI Accession NP_067258
UniProt P07750

Interleukin 4 (IL4) 抗體使用細節(jié)

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使用細節(jié)

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應(yīng)用備注 Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of mIL-4 (7.5?ng/mL), aconcentration of 0.25-0.40?μg/mL of this antibody is required. ELISA: (Indirect): To detect mIL-4 by indirect ELISA (using 100 μL/well antibody solution) aconcentration of 0.5-2.0?μg/mL of this antibody is required. This antigen affinity purifiedantibody, in conjunction with compatible secondary reagents, allows the detection of atleast 0.2-0.4?ng/well of recombinant mIL-4. (Sandwich): To detect mIL-4 by sandwich ELISA (using 100 μL/well antibody solution) aconcentration of 0.5-2.0?μg/mL of this antibody is required. This antigen affinity purifiedantibody, in conjunction with Biotinylated Anti-Murine IL-4 (PP011B) as a detectionantibody, allows the detection of at least 0.2-0.4?ng/well of recombinant mIL-4. Western Blot: To detect mIL-4 by Western Blot analysis this antibody can be used at a concentration of
限制 For Research Use only

貯存及處理

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貯存及處理

顯示 隱藏
溶解方式 Restore in sterile water to a concentration of 0.1-1.0?mg/mL.
濃度 1.0 mg/mL
緩沖液 PBS,?pH 7.2 without preservatives.
儲存液 Without preservative
注意事項 Avoid repeated freezing and thawing. Centrifuge vial prior to opening!
儲存條件 4 °C/-20 °C
儲存方法 Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.

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