IL10抗體

抗原
Interleukin 10 (IL10)
別名 CSIF, GVHDS, IL-10, IL10A, TGIF, Il-10, IL10X
適用
其他選擇 Rat ((Rattus))
Human(235),Murine (135),Rat ((Rattus)) (49),Pig (Porcine) (26),Chicken (12),Dog (Canine) (12),Bovine (9),Horse (Equine) (4),Sheep (Ovine) (3),Goat (2),Rabbit (1),Wild boar (Sus scrofa) (1)
宿主
其他選擇 Rabbit
Rat(137),Rabbit (131),Mouse (83),Sheep (6),Goat (4),Chicken (3)
克隆類型
多克隆
標(biāo)記
其他選擇 非結(jié)合性
Biotin(58),PE (14),FITC (12),APC (5),Alexa Fluor 488 (4),Alexa Fluor 647 (3),HRP (3),Alexa Fluor 350 (2),Alexa Fluor 555 (2),Cy3 (2),Cy5 (2),Cy5.5 (2),Cy7 (2),PerCP,Cy5.5 (2),Alkaline Phosphatase (AP) (1),Magnetic Particles (1),PE-Cy7 (1),Sepharose (1)
應(yīng)用范圍
其他選擇 Enzyme Immunoassay (EIA), Functional Studies (Func), Western Blotting (WB)
Western Blotting (WB)(213),ELISA (204),Flow Cytometry (FACS) (90),Immunohistochemistry (IHC) (42),Neutralization (Neut) (39),Functional Studies (Func) (32),Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (30),Enzyme Immunoassay (EIA) (29),Immunoprecipitation (IP) (29),Immunofluorescence (Paraffin-embedded Sections) (IF (p)) (19),Immunohistochemistry (Frozen Sections) (IHC (fro)) (17),ELISpot (12),Immunocytochemistry (ICC) (12),Blocking Antibody (Inhibition) (8),Immunofluorescence (IF) (8),Radioimmunoassay (RIA) (7),Intracellular Staining (ICS) (6),Immunoassay (IA) (5),ELISA (Detection) (4),Immunohistochemistry (Frozen Paraffin-embedded Sections) (IHC (frpe)) (3),Dot Blot (DB) (2),ELISA (Capture) (2),Transfected cell culture (TCC) (2),ELISpot (Detection) (1),Proximity Ligation Assay (PLA) (1)
[Pubmed] 2 references available
規(guī)格 0.1 mg
發(fā)貨至 中國 (更改)
發(fā)貨時間 29至35個工作日

IL10抗體

產(chǎn)品細(xì)節(jié)

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產(chǎn)品細(xì)節(jié)

顯示 隱藏
免疫原 Highly pure (>98%) recombinant Rat IL-10.
特異性 Recognizes Rat IL10. Other species not tested.
純化方法 Affinity Chromatography employing immobilized Rat IL-10 matrix.

目標(biāo)詳細(xì)情況

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目標(biāo)詳細(xì)情況

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別名 Interleukin-10 / IL10
背景 Interleukins (ILs) are a large group of cytokines that are produced mainly by leukocytes, although some are produced by certain phagocytes and auxiliary cells. ILs have a variety of functions, but most function to direct other immune cells to divide and differentiate. Each IL acts on a specific, limited group of cells through a receptor specific for that IL. Human IL10 is a non glycosylated polypeptide consisting of 178 amino acids. There is 73?% homology between the human and mouse IL10 proteins, however, the human IL10 acts on both human and mouse target cells, while the mouse IL10 has species specific activity. The cellular sources of IL10 are CD4+ T cells and T cell clones, thymocytes, B cells and B cell lymphomas, macrophages, mast cell lines and keratinocytes. IL10 will stimulate the growth of stem cells, mast cells and thymocytes. IL10 enhances cytotoxic T cell development, and costimulates B cell differentiation and immunoglobulin secretion. IL10 inhibits cytokine production by macrophages and suppresses macrophage class II MHC expression. The human IL10 gene is on human chromosome 1.Synonyms: CSIF, Cytokine synthesis inhibitory factor, IL-10, TGIF
基因ID 25325
NCBI Accession NP_036986
UniProt P29456

使用細(xì)節(jié)

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使用細(xì)節(jié)

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應(yīng)用備注 Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of RatIL-10 (30.00?ng/mL), a concentration of 0.45-0.80?μg/mL of this antibody is required. ELISAIndirect: To detect Rat IL-10 by indirect ELISA (using 100 μL/well antibody solution) aconcentration of 0.5-2.0?μg/mL of this antibody is required. This antigen affinity purifiedantibody, in conjunction with compatible secondary reagents, allows the detection of atleast 0.2-0.4?ng/well of recombinant Rat IL-10. Sandwich: To detect Rat IL-10 by sandwich ELISA (using 100 μL/well antibody solution) aconcentration of 0.5-2.0?μg/mL of this antibody is required. This antigen affinity purifiedantibody, in conjunction with Biotinylated Anti-Rat IL-10 (PP1115B1 or PP1115B2) as a
限制 For Research Use only

貯存及處理

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貯存及處理

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溶解方式 Restore in sterile water to a concentration of 0.1-1.0?mg/mL.
緩沖液 PBS,?pH 7.2 without preservatives.
儲存液 Without preservative
注意事項(xiàng) Avoid repeated freezing and thawing. Centrifuge vial prior to opening.
儲存條件 4 °C/-20 °C
儲存方法 The lyophilized antibody is stable at -20 °C for one year from despatch. The reconstituted antibody is stable for two weeks at 2-8 °C. Frozen aliquots are stable for six months when stored at -20 °C.
有效期 12 months

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