CD80抗體

抗原
CD80 (CD80)
別名 CD8, Leu2, MAL, p32, B71, Cd28l, Ly-53, Ly53, MIC17, TSA1, B7, B7-1, B7.1, BB1, CD28LG, CD28LG1, LAB7
抗原表位
其他選擇 AA 1-242
Extracellular Domain(10),AA 1-242 (9),C-Term (7),AA 258-288 (6),AA 1-245 (4),AA 1-288 (4),AA 57-71 (4),Internal Region (3),N-Term (3),AA 35-288 (2),Extracellular (2),AA 1-224 (1),AA 37-245 (1),AA 45-63 (1)
適用
其他選擇 Human
Human(238),Murine (194),Rat ((Rattus)) (43),Dog (Canine) (14),Chicken (4),Bovine (4),Baboon (2),Monkey (1)
宿主
其他選擇 Rabbit
Mouse(211),Rat (91),Rabbit (84),Hamster (63),Armenian Hamster (12),Goat (8)
克隆類型
多克隆
標記
其他選擇 非結(jié)合性
PE(57),FITC (52),Biotin (48),APC (15),Cy5,PE (6),Alexa Fluor 647 (5),HRP (4),PerCP (4),SPRD (4),Alexa Fluor 350 (3),Alexa Fluor 488 (3),Alexa Fluor 555 (3),Allophycocyanin (3),Cy3 (3),Cy5 (3),Cy5.5 (3),Cy7 (3),PE-Cy5 (3),PE,Cy5 (2),Alkaline Phosphatase (AP) (1),DyLight 488 (1),DyLight 550 (1),DyLight 594 (1),DyLight 650 (1),DyLight 755 (1),Magnetic Particles (1),Sepharose (1)
zui低檢測濃度 ≤ 0.039 ng/well
應用范圍
其他選擇 ELISA
Flow Cytometry (FACS)(309),Western Blotting (WB) (123),Immunoprecipitation (IP) (84),ELISA (50),Immunofluorescence (IF) (38),Immunohistochemistry (IHC) (37),Immunofluorescence (Paraffin-embedded Sections) (IF (p)) (30),Functional Studies (Func) (25),Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (24),Immunohistochemistry (Frozen Sections) (IHC (fro)) (23),Immunocytochemistry (ICC) (19),Immunohistochemistry (Acetone-fixed) (IHC (af)) (8),Enzyme Immunoassay (EIA) (7),Blocking Antibody (Inhibition) (2),Immunoassay (IA) (2),Blocking Reagent (BR) (1),Dot Blot (DB) (1),Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (1),Immunohistochemistry (Frozen Paraffin-embedded Sections) (IHC (frpe)) (1),Immunostaining (IS) (1),Neutralization (Neut) (1),Proximity Ligation Assay (PLA) (1)
[Pubmed] 1 reference available
規(guī)格 100 μg
發(fā)貨至 中國 (更改)

CD80抗體

產(chǎn)品細節(jié)

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產(chǎn)品細節(jié)

顯示 隱藏
亞型 IgG
特異性 Human CD80 / B7-1
產(chǎn)品特性 Produced in rabbits immunized with purified, recombinant Human CD80 / B7-1 (rh CD80 / B7-1, Met 1-Asn 242, NP_005182.1). Total IgG was purified by Protein A affinity chromatography.
純化方法 Affinity Purified
過濾 0.2 μm filtered

目標詳細情況

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目標詳細情況

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別名 CD80
背景 The B-lymphocyte activation antigen B7-1 (referred to as B7), also known as CD80, is a member of cell surface immunoglobulin superfamily and is expressed on the surface of antigen-presenting cells including activated B cells, macrophages and dendritic cells. As costimulatory ligands, B7-1 which exists predominantly as dimer and the related protein B7-2, interact with the costimulatory receptors CD28 and cytotoxic T lymphocyte- associated antigen 4 (CTLA-4) expressed on T cells, and thus constitute one of the dominant pathways that regulate T cell activation and tolerance, cytokine production, and the generation of CTL. The B7/CD28/CTLA4 pathway has the ability to both positively and negatively regulate immune responses. CD80 is thus regarded as promising therapeutic targets for autoimmune diseases and various carcinomas.
NCBI Accession NP_005182.1
研究領(lǐng)域 Stem Cells, Hematopoietic Progenitors, Adaptive Immunity, CD Antigens, Surface Receptors of Immune Cells

使用細節(jié)

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使用細節(jié)

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應用備注 ELISA ELISA: 0.5-1 μg/mL This antibody can be used at 0.5-1 μg/mL with the appropriate secondary reagents to detect Human CD80 / B7-1. The detection limit for Human CD80 / B7-1 is approximay ≤ 0.039 ng/well.
限制 For Research Use only

貯存及處理

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貯存及處理

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緩沖液 PBS with 5% trehalose
儲存液 Without preservative
注意事項 Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
儲存條件 4 °C/-20 °C/-80 °C
儲存方法 This antibody can be stored at 2 °C-8 °C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 °C to -70 °C.
有效期 12 months

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