著絲粒ZWILCH蛋白抗體

英文名稱 ZWILCH
中文名稱 著絲粒ZWILCH蛋白抗體
別    名 hZwilch; KNTC1AP; MGC111034; Protein zwilch homolog; ZWILC_HUMAN; zwilch;y ZWILCH protein; Zwilch, kinetochore associated, homolog.
  
說 明 書 0.1ml  0.2ml  
研究領域 細胞生物  免疫學  
抗體來源 Rabbit
克隆類型 Polyclonal
交叉反應 Human, Mouse, Rat, Horse, Rabbit, 
產品應用 WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:50-200 （石蠟切片需做抗原修復） 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 67kDa
細胞定位 細胞核 
性    狀 Lyophilized or Liquid
濃    度 1mg/1ml
免 疫 原 KLH conjugated synthetic peptide derived from human ZWILCH
亞    型 IgG
純化方法 affinity purified by Protein A
儲 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存條件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

PubMed PubMed
產品介紹 background:
Zwilch is the human homolog of the Drosophila Zwilch protein. The Drosophila Zwilch forms a complex with both ROD Rough Deal) and ZWINT (Zeste-White 10, also designated ZW10) proteins. This complex is important for chromosome segregation because it recruits cytoplasmic Dynein to the kinetochore and plays a crucial role in the spindle checkpoint. The role of Zwilch in complex is thought to be evolutionarily conserved because the human homologs of Zwilch, ZWINT and ROD coimmunoprecipitate in a human cell line called HeLa. The human Zwilch, ZWINT and ROD complex localizes to the kinetochores at prometaphase. Mutations were discovered in Zwilch, ZWINT and ROD during a screen for mutations in alleles encoding putative chromosome instability genes in cases of human colorectal cancer. These mutations may contribute in part to the chromosomal instability phenotype of colorectal tumor cells.

Function:
Essential component of the mitotic checkpoint, which prevents cells from prematurely exiting mitosis. Required for the assembly of the dynein-dynactin and MAD1-MAD2 complexes onto kinetochores. xy-0311R-AF488    Rabbit Anti-Biotin/Alexa Fluor 488Alexa Fluor 488標記的兔抗生物素抗體IgG
xy-0311R-AF555    Rabbit Anti-Biotin/Alexa Fluor 555Alexa Fluor 555標記的兔抗生物素抗體IgG
xy-0311R-AF647    Rabbit Anti-Biotin/Alexa Fluor 647Alexa Fluor 647標記的兔抗生物素抗體IgG
xy-0311R-xyE-xyy5.5    Rabbit Anti-Biotin/PE-Cy5.5PE-Cy5.5標記的兔抗生物素抗體IgG
xy-0311R-xyE-xyy7    Rabbit Anti-Biotin/PE-Cy7PE-Cy7標記的兔抗生物素抗體IgG
xy-0311R-Bio    Rabbit Anti-Biotin/Bio生物素標記的兔抗生物素抗體IgG
xy-0311R-Axy    Rabbit Anti-Biotin/AP堿性磷酸酶（AP）標記的兔抗生物素抗體IgG
xy-0311R-FITxy    Rabbit Anti-Biotin/FITCFITC標記的兔抗生物素抗體IgG
xy-0311R-Golxy    Rabbit Anti-Biotin/Gold膠體金標記的兔抗生物素抗體IgG
xy-0311R-RBITxy    Rabbit Anti-Biotin/RBITC羅丹明標記的兔抗生物素抗體IgG
xy-0326R-RBITxy    Rabbit Anti-Bov IgG/RBITC羅丹明標記的兔抗牛IgG
xy-0326R-Axy    Rabbit Anti-Bov IgG/AP堿性磷酸酶（AP）標記的兔抗牛IgG
xy-0326R-xyE-xyy7    Rabbit Anti-Bov IgG/PE-Cy7PE-Cy7標記的兔抗牛IgG
xy-0326R-Golxy    Rabbit Anti-Bov IgG/Gold膠體金標記的兔抗牛IgG
xy-0326R-Bio    Rabbit Anti-Bov IgG/Bio生物素標記的兔抗牛IgG
xy-0326R-xyy3    Rabbit Anti-Bov IgG/Cy3Cy3標記的兔抗牛IgG
xy-0326R-xyy5    Rabbit Anti-Bov IgG/Cy5Cy5標記的兔抗牛IgG
xy-0326R-xyy5.5    Rabbit Anti-Bov IgG/Cy5.5Cy5.5標記的兔抗牛IgG
xy-0326R-xyy7    Rabbit Anti-Bov IgG/Cy7Cy7標記的兔抗牛IgG
xy-0326R-xyE    Rabbit Anti-Bov IgG/PEPE標記的兔抗牛IgG
xy-0326R-xyE-xyy3    Rabbit Anti-Bov IgG/PE-Cy3PE-Cy3標記的兔抗牛IgG
xy-0326R-xyE-xyY5    Rabbit Anti-Bov IgG/PE-CY5PE-CY5標記的兔抗牛IgG
xy-0326R-Axyxy    Rabbit Anti-Bov IgG/APCAPC標記的兔抗牛IgG
xy-0326R-AF350    Rabbit Anti-Bov IgG/Alexa Fluor 350Alexa Fluor 350標記的兔抗牛IgG
xy-0326R-AF488    Rabbit Anti-Bov IgG/Alexa Fluor 488Alexa Fluor 488標記的兔抗牛IgG
xy-0326R-AF555    Rabbit Anti-Bov IgG/Alexa Fluor 555Alexa Fluor 555標記的兔抗牛IgG
xy-0327R-xyE-xyy3    Rabbit Anti-Bovine IgM/PE-Cy3PE-Cy3標記的兔抗牛IgM
xy-0327R-xyE-xyY5    Rabbit Anti-Bovine IgM/PE-CY5PE-CY5標記的兔抗牛IgM
xy-0327R-Axyxy    Rabbit Anti-Bovine IgM/APCAPC標記的兔抗牛IgM
xy-0327R-AF350    Rabbit Anti-Bovine IgM/Alexa Fluor 350Alexa Fluor 350標記的兔抗牛IgM
xy-0327R-AF647    Rabbit Anti-Bovine IgM/Alexa Fluor 647Alexa Fluor 647標記的兔抗牛IgM
xy-0327R-xyy3    Rabbit Anti-Bovine IgM/Cy3Cy3標記的兔抗牛IgM
xy-0327R-xyy5    Rabbit Anti-Bovine IgM/Cy5Cy5標記的兔抗牛IgM
xy-0327R-xyy5.5    Rabbit Anti-Bovine IgM/Cy5.5Cy5.5標記的兔抗牛IgM