Recombinant Methylmalonyl Coenzyme A Epimerase (MCEE) 

甲基丙二酰輔酶A異構(gòu)酶(MCEE)重組蛋白

[ PROPERTIES ]

Residues: Gln37~Ala176

Host: Two N-terminal Tags, His-tag and T7-tag

Accession: Q96PE7

Host: E. coli

Subcellular Location: Mitochondrion

Purity: >95%

Endotoxin Level: <1.0EU per 1μg

(determined by the LAL method).

Formulation: Supplied as lyophilized form in 20mM Tris,150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT,0.01% sarcosyl, 5% trehalose, and preservative.

Predicted isoelectric point: 8.0

Predicted Molecular Mass: 18.8kDa

Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

[ USAGE ]

Reconstitute in sterile ddH₂O.

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles.

             Store at 2-8oC for one month.

             Aliquot and store at -80oC for 12 months.

Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

[SEQUENCE ]

The sequence of the target protein is listed below.

QVTG SVWNLGRLNH VAIAVPDLEK AAAFYKNILG AQVSEAVPLP EHGVSVVFVNLGNTKMELLH PLGRDSPIAG FLQKNKAGGM HHICIEVDNI NAAVMDLKKK KIRSLSEEVKIGAHGKPVIF LHPKDCGGVL VELEQA

[  IDENTIFICATION  ]

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