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上海橋星貿(mào)易有限公司
主營產(chǎn)品： 進口透析袋,密理博ECL發(fā)光液,B27無血清培養(yǎng)基,Gibco膠原酶 |
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聯(lián)系電話
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上海橋星貿(mào)易有限公司
主營產(chǎn)品： 進口透析袋,密理博ECL發(fā)光液,B27無血清培養(yǎng)基,Gibco膠原酶 |
聯(lián)系電話
M2206 Klenow I Promega試劑? 原裝*，質(zhì)量保證，現(xiàn)貨供應(yīng)
規(guī)格：500u
M2206 Klenow I Promega試劑? 產(chǎn)品描述：
其它組分：
DNA Polymerase 10X Buffer【子貨號：M195A，包裝：1 x 1ml，，運保溫度：-20℃】
DNA Polymerase I Large (Klenow) Fragment【子貨號：M220C，包裝：1 x 500u，，運保溫度：-20℃】
描述：
說明 |
| DNA Polymerase I Large (Klenow) Fragment (DNA 聚合酶I 大(Klenow) 片段) 是DNA 依賴的DNA 聚合酶，它沒有完整的E. coli DNA Polymerase I 所具有的 5` → 3` 外切酶活性，但保留了5` → 3` 聚合酶、3` → 5` 外切酶及鏈置換活性。該酶是DNA 聚合酶I 的C- 末端片段，68kDa。Klenow 片段5` → 3` 聚合酶活性經(jīng)常被用于以非標記的或標記的dNTPs 補平5`- 突出端、單鏈或雙鏈DNA 模板測序、合成寡核苷酸進行體外突變、cDNA第二鏈合成以及制備單鏈DNA探針。3` → 5` 外切酶活性可用于從3`- 突出端產(chǎn)生平末端。
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特點 |
| • 靈活性：DNA Polymerase I Large (Klenow) Fragment 可用于多種分子實驗。在多種Promega 1× 內(nèi)切酶緩沖液中它都能保持活性。 |
原廠資料：
DNA Polymerase I Large (Klenow) Fragment is a DNA-dependent DNA polymerase that lacks the 5´→3´ exonuclease activity of intact E. coli DNA Polymerase I but retains its 5´→3´ polymerase, 3´→5´ exonuclease and strand displacement activities. The enzyme is a 68kDa C-terminal fragment of DNA Polymerase I. The 5´→3´ polymerase activity of Klenow Fragment can be used to fill in 5´-protruding ends with unlabeled or labeled dNTPs, to sequence single- or double-stranded DNA templates, for in vitro mutagenesis using synthetic oligonucleotides, for cDNA second-strand synthesis and to generate single-stranded DNA probes. The 3´→5´ exonuclease activity can be used to generate blunt ends from a 3´-overhang.
Features - Benefits
Flexible: DNA Polymerase I Large (Klenow) Fragment may be used in a variety of molecular applications. It is also active in many Promega 1X restriction enzyme buffers.
May Be Heat-Inactivated: DNA Polymerase I Large (Klenow) Fragment is inactivated by heating at 75°C for 10 minutes.
Provided with 10X Reaction Buffer: 500mM Tris-HCl (pH 7.2 at 25°C), 100mM MgSO4, 1mM DTT.
Choose Your Configuration: Learn more about our custom options for this product at: www.promega。。ｃｏｍ/custom/
Applications
Filling-in or labeling 3´ ends of 5´ overhanging double-stranded DNA.
Second-strand cDNA synthesis.
DNA sequencing by the dideoxy method.
References
Anderson, S. et al. (1980) Nucl. Acids Res. 8, 1731–43.
Sanger, F. et al. (1977) Proc. Natl. Acad. Sci. USA 74, 5463–7.
Wallace, R.B. et al. (1980) Science 209, 1396–400.
Houdebine, L.M. (1976) Nucl. Acids Res. 3, 615–30.
Feinberg, A.P. and Vogelstein, B. (1983) Anal. Biochem. 132, 6–13.
Tabor, S. and Struhl, K. (1987) In: Current Protocols in Molecular Biology, Ausubel, F.M. et al., eds., John Wiley and Sons, New York, NY.
Joyce, C.M. and Grindley, N.D.F. (1983) Proc. Natl. Acad. Sci. USA 80, 1830–4.
M2206 Klenow I Promega試劑? 相關(guān)產(chǎn)品列表：
| Promega 系列 | ||
| 序號 | 貨號 名稱 | 規(guī)格 |
| 1 | M1801 T4連接酶 | 100U |
| 2 | M1804 T4連接酶 | 500U |
| 3 | M1701 M-MLV | 10000U |
| 4 | M1705 M-MLV | 50000U |
| 5 | M5101 AMV | 300U |
| 6 | M5108 AMV | 1000U |
| 7 | M7741 Pfu | 100U |
| 8 | M7745 Pfu | 500U |
| 9 | C1101 Olig(dt)15 Primer | 20ug |
| 10 | M3001 Go-Taq | 100U |
| 11 | M3005 Go-Taq | 500U |
| 12 | M3008 Go-Taq | 2500U |
| 13 | U1511 dNTP | 200μl |
| 14 | U1515 dNTP | 1000μl |
| 15 | A1360 pGEM-T easy | 1.2ug |
| 16 | A3600 pGEM-T | 1.2ug |
| 17 | C1181 Random Primers | 20μg |
| 18 | N2511 Recombinant RNasin | 2500U |
| 19 | N2515 Recombinant RNasin | 10000U |
| 20 | N2611 RNasin Plus | 2500U |
| 21 | N2615 RNasin Plus | 10000U |
| 22 | N2111 RNasin | 2500U |
| 23 | N2115 RNasin | 10000U |
| 24 | M1871 TdT | 300U |
| 25 | M1875 TdT | 1500U |
| 26 | M2825 CIAP | 1000U |
| 27 | M1821 CIAP | 1000U |
| 28 | M2201 Klenow I | 150U |
| 29 | M2206 Klenow I | 500U |
| 30 | M4211 T4 DNA Polymerase | 100U |
| 31 | M4215 T4 DNA Polymerase | 500U |
| 32 | M4101 T4 Polynucleotide Kinase | 100U |
| 33 | M4103 T4 Polynucleotide Kinase | 1000U |
