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上海橋星貿(mào)易有限公司
主營產(chǎn)品： 進口透析袋,密理博ECL發(fā)光液,B27無血清培養(yǎng)基,Gibco膠原酶 |
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聯(lián)系電話
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上海橋星貿(mào)易有限公司
主營產(chǎn)品： 進口透析袋,密理博ECL發(fā)光液,B27無血清培養(yǎng)基,Gibco膠原酶 |
聯(lián)系電話
M7745 Pfu DNA Polymerase 500u Promega試劑
規(guī)格：500U
M7745 Pfu DNA Polymerase 500u Promega試劑 產(chǎn)品描述：
其它組分：
Pfu DNA Polymerase【子貨號：M774B，包裝：1 x 500u，，運保溫度：-20℃】
Pfu 10X Reaction Buffer w/20mM MgSO4【子貨號：M776A，包裝：3 x 1.2ml，，運保溫度：-20℃】
描述：
說明 | Pfu DNA Polymerase 是一種從強烈熾熱球菌(Pyrococcus furiosus ) 中分離得到的熱穩(wěn)定性酶，分子量大約為90kDa。該酶在75℃時可進行DNA 復制，在鎂離子存在的條件下可催化核苷酸沿5` → 3` 方向發(fā)生聚合反應，形成雙鏈DNA。 Pfu DNA 聚合酶也具有3` → 5` 外切酶( 校正) 活性。當聚合反應發(fā)生堿基錯配時，聚合酶的校正活性可將錯配的堿基切除。因而，推薦Pfu DNA 聚合酶用于高保真的PCR 反應和引物的延伸反應。Pfu DNA 聚合酶產(chǎn)生的PCR產(chǎn)物為平端。
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特點 | . 高保真：在所有熱穩(wěn)定性聚合酶中Pfu DNA 聚合酶的錯誤幾率zui低。 . 完備：：提供含有20mM MgSO4 的10× 反應緩沖液。 . *：Promega 公司的PCR 系統(tǒng)，酶和試劑被證明在PCR中能得到可靠高效的結(jié)果。 |
原廠資料：
Pfu DNA Polymerase is a thermostable enzyme of approximay 90kDa isolated from Pyrococcus furiosus. The enzyme replicates DNA at 75°C, catalyzing the polymerization of nucleotides into duplex DNA in the 5´→3´ direction in the presence of magnesium. Pfu DNA Polymerase also possesses 3´→5´ exonuclease (proofreading) activity. Base misinsertions that may occur during polymerization are rapidly excised by the proofreading activity of the polymerase. Consequently, PfuDNA Polymerase is recommended for use in PCR and primer extension reactions that require high-fidelity synthesis. Pfu DNA Polymerase-generated PCR fragments are blunt-ended.
Pfu DNA Polymerase 10X Reaction Buffer with MgSO4: 200mM Tris-HCl (pH 8.8 at 25°C), 100mM KCl, 100mM (NH4)2SO4, 20mM MgSO4, 1.0% Triton® X-100 and 1mg/ml nuclease-free BSA.
PCR Satisfaction Guarantee
Promega's PCR Systems, enzymes and reagents are proven in PCR to ensure reliable, high performance results. Your success is important to us. Our products are backed by a worldwide team of Technical Support scientists. Please contact them for applications or technical assistance. If you are not compley satisfied with any Promega PCR product we will send a replacement or refund your account.That's Our PCR Guarantee!
Product must be within expiration date and have been stored and used in accordance with product literature. See Promega Product Insert for specific tests preformed.
Pfu DNA Polymerase is recommended for use in PCR, primer extension reactions and other applications that demand high fidelity.
For more information, see the Protocols & Applications Guide.
相關(guān)產(chǎn)品列表：
| Promega 系列 | ||
| 序號 | 貨號 名稱 | 規(guī)格 |
| 1 | M1801 T4連接酶 | 100U |
| 2 | M1804 T4連接酶 | 500U |
| 3 | M1701 M-MLV | 10000U |
| 4 | M1705 M-MLV | 50000U |
| 5 | M5101 AMV | 300U |
| 6 | M5108 AMV | 1000U |
| 7 | M7741 Pfu | 100U |
| 8 | M7745 Pfu | 500U |
| 9 | C1101 Olig(dt)15 Primer | 20ug |
| 10 | M3001 Go-Taq | 100U |
| 11 | M3005 Go-Taq | 500U |
| 12 | M3008 Go-Taq | 2500U |
| 13 | U1511 dNTP | 200μl |
| 14 | U1515 dNTP | 1000μl |
| 15 | A1360 pGEM-T easy | 1.2ug |
| 16 | A3600 pGEM-T | 1.2ug |
| 17 | C1181 Random Primers | 20μg |
| 18 | N2511 Recombinant RNasin | 2500U |
| 19 | N2515 Recombinant RNasin | 10000U |
| 20 | N2611 RNasin Plus | 2500U |
| 21 | N2615 RNasin Plus | 10000U |
| 22 | N2111 RNasin | 2500U |
| 23 | N2115 RNasin | 10000U |
| 24 | M1871 TdT | 300U |
| 25 | M1875 TdT | 1500U |
| 26 | M2825 CIAP | 1000U |
| 27 | M1821 CIAP | 1000U |
| 28 | M2201 Klenow I | 150U |
| 29 | M2206 Klenow I | 500U |
| 30 | M4211 T4 DNA Polymerase | 100U |
| 31 | M4215 T4 DNA Polymerase | 500U |
| 32 | M4101 T4 Polynucleotide Kinase | 100U |
| 33 | M4103 T4 Polynucleotide Kinase | 1000U |
