目錄:愛必信（上海）生物科技有限公司>>細胞生物學>>重組蛋白>> abs00919Recombinant Human IFN-ω
| 供貨周期 | 一周 | 規(guī)格 | 20ug |
|---|
| 概述 | |
|---|---|
| 種屬 | Human |
| 形態(tài) | Lyophilized from a 0.2 µm filtered concentrated solution in PBS, pH 7.4. |
| 來源 | Escherichia coli. |
| 氨基酸序列 | CDLPQNHGLL SRNTLVLLHQ MRRISPFLCL KDRRDFRFPQ EMVKGSQLQK AHVMSVLHEM LIFSLFHT ERSSAAWNMT LLDQLHTGLH LQHLETCL LQVVGEGESA GAISSPALTL RRYFQGIRVY LKEKKYSDCA WEVVRMEIMK SLFLSTNMQE RLRSKDRDLG SS |
| 內(nèi)毒素水平 | Less than 1 EU/μg of Recombinant HumanIFN-ω as determined by LAL method. |
| 活性 | Fully biologically active when compared to standard. The ED50 as determined by a chemotaxis bioassay using human TF-1 cells is less than 0.01 ng/ml, corresponding to a specific activity of > 1.0 × 108 IU/mg. |
| Accession # | P05000 |
| 預期分子量 | Approximay 20.0 kDa, containing 172 amino acid residues with two conserved disulfide bonds. |
| 性能 | |
|---|---|
| 溶解方法 | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
| 保存方法 | This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles. |
| 純度 | > 97 % by SDS-PAGE and HPLC analyses. |
| Gene IDs | 3467 |
| 參考文獻 | |
|---|---|
| 1. Yang LM, Xue QH, Sun L, et al. 2007. J Interferon Cytokine Res, 27: 119-27.<br/>2. Liu L, Leaman DW, Bixby JA, et al. 1996. Biochim Biophys Acta, 1294: 55-62.<br/>3. Adolf GR. 1990. Virology, 175: 410-7.<br/>4. Benoit P, Maguire D, Plavec I, et al. 1993. J Immunol, 150: 707-16. | |
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