目錄:愛必信(上海)生物科技有限公司>>細胞生物學(xué)>>重組蛋白>> abs00914Recombinant Human IFN-α2b, Ecoli.
供貨周期 | 一周 | 規(guī)格 | 20ug |
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概述 | |
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形態(tài) | Lyophilized from a 0.2 µm filtered solution in PBS, pH 7.4. |
來源 | Escherichia coli. |
氨基酸序列 | MCDLPQTHSL GSRRTLMLLA QMRRISLFSC LKDRHDFGFP QEEFGNQFQK AETIPVLHEM IIFNLFST KDSSAAWDET LLDKFYY LNDLEACV IQGVGVTETP LMKEDSILAV RKYFQRITLY LKEKKYSPCA WEVVRAEIMR SFSLSTNLQE SLRSKE |
內(nèi)毒素水平 | Less than 1 EU/µg of Recombinant HumanIFN-α2b, Ecoli. as determined by LAL method. |
活性 | Fully biologically active when compared to standard. The specific activity determined by an anti-viral assay is no less than 1.0 × 108 IU/mg. |
Accession # | P01563 |
預(yù)期分子量 | Approximay 19.4 kDa, a single non-glycosylated polypeptide chain containing 166 amino acids. |
性能 | |
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溶解方法 | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
保存方法 | This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles. |
純度 | > 96 % by SDS-PAGE and HPLC analyses. |
Gene IDs | 3440 |
參考文獻 | |
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1. Tarhini AA, Gogas H, Kirkwood JM. 2012. J Immunol, 189: 3789-93.<br/>2. Tohyama M, Yang L, Hanakawa Y, et al. 2012. J Invest Dermatol, 132: 1933-5.<br/>3. Corssmit EP, Heijligenberg R, Hack CE, et al. 1997. Clin Exp Immunol, 107: 359-63.<br/>4. Corssmit EP, de Metz J, Sauerwein HP, et al. 2000. J Interferon Cytokine Res, 20: 1039-47. |