概述 |
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描述 | Recombinant Mouse Granulocyte-Macrophage Colony-Stimulating Factor is produced by our E.coli expression system and the target gene encoding Ala18-Lys141 is expressed. |
別名 | GM-CSF蛋白; CSF2蛋白;Granulocyte-macrophage colony-stimulating factor,Csf2,GM-CSF,Colony-stimulating factor,Csfgm, REF: C1002 |
種屬 | mouse |
來源 | Escherichia coli. |
分子量 | 14.8kDa |
形態(tài) | Lyophilized |
性能 |
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純度 | Greater than 98% as determined by reducing SDS-PAGE. |
內(nèi)毒素水平 | Less than 0.03EU/μg as determined by LAL test. |
活性 | Measured in a cell proliferation assay using FDC-P1 cells. The ED50 for this effect is 0.1 - 0.3 ng/mL. |
溶解方法 | Recombinant Mouse GM-CSF/CSF2 Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in ddH2O. It is recommended to dissolve and dilute with a complete medium containing 10% calf serum when used for cell culture.Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
儲存/保存方法 | Recombinant Mouse GM-CSF/CSF2 Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.Reconstituted protein solution can be stored at 4-7°C for 2-7 days.Aliquots of reconstituted samples are stable at < -20°C for 3 months. |
靶標 |
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背景說明 | Granulocyte-macrophage colony-stimulating factor is an enzyme that in mouse is encoded by the Csf2 gene, belongs to the GM-CSF family.CSF2 is a Cytokine that stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes |
Accession | P01587 |
實驗結(jié)果圖 |
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| rmGM-CSF(10ng/ml)培養(yǎng)的小鼠樹突狀細胞 |
溫馨提示:本產(chǎn)品僅作科研實驗使用,不支持臨床等研究 |
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