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當(dāng)前位置：> 供求商機(jī)> a549d-nfis——Human NF-κB-SEAP & IRF-Luc Reporter lung carcinoma
當(dāng)前位置：> 供求商機(jī)> a549d-nfis——Human NF-κB-SEAP & IRF-Luc Reporter lung carcinoma
貨物所在地：北京北京市
更新時(shí)間：2024-03-27 13:43:25
有效期：2024年3月27日 -- 2025年3月28日
已獲點(diǎn)擊：43
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A549-Dual™ cells are adherent epithelial cells that have been derived from the human A549 lung carcinoma cell line by stable integration of two inducible reporter constructs. The A549 cell line is a well-characterized cellular model for asthma, allergies and respiratory infections.
A549-Dual™ cells express a secreted embryonic alkaline phosphatase (SEAP) reporter gene under the control of the IFN-β minimal promoter fused to five NF-κB binding sites.
A549-Dual™ cells also express the Lucia luciferase gene, which encodes a secreted luciferase, under the control of an ISG54 minimal promoter in conjunction with five IFN-stimulated response elements.
As a result, A549-Dual™ cells allow to simultaneously study the NF-κB pathway, by assessing the activity of SEAP, and the interferon regulatory factor (IRF) pathway, by monitoring the activity of Lucia luciferase.
Both reporter proteins are readily measurable in the cell culture supernatant when using QUANTI-Blue™ Solution, a SEAP detection reagent, and QUANTI-Luc™ 4 Lucia/Gaussia, a Lucia and Gaussia luciferase detection reagent.
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