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當(dāng)前位置:> 供求商機(jī)> 350101-5ML——胺基活化瓊脂糖凝膠4HF

[供應(yīng)]350101-5ML——胺基活化瓊脂糖凝膠4HF

貨物所在地:北京北京市

更新時(shí)間:2024-04-08 10:46:21

有效期:2024年4月8日 -- 2025年4月9日

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Amine-activated agarose beads can be used for the immobilization of carboxyl containing or aldehyde containing molecules.

胺基活化瓊脂糖層析填料

Product Introduction:Amine-activated agarose beads can be used for the immobilization of carboxyl containing or aldehyde containing molecules. The coupling chemistry is carbodiimide-based and is a successful and well-documented technique. Amine-activated SepFast forms a chemically stable amide linkage with ligands containing carboxyl groups. It has a long spacer (9 atoms). The pre-activated agarose base matrix can be readily employed to make various custom affinity chromatography adsorbents for both small scale and large scale purification applications.

胺基特性.png

Product Properties:

Amine-activated SepFast 4 High Flow (4HF) is made of highly cross-linked 4% beaded agarose. It shows high mechanical rigidity allowing high flow throughput with reduced back pressure. 

Agarose has long been used for chromatographic separations due to its excellent hydrophilic and low non-specific-binding nature. The particles have an open pore structure with excellent mass transfer properties to large protein molecules. 

The base matrix is activated by attaching a long hydrophilic chain with an amine group in the end. They react with either carboxyl containing ligands in the presence of a carbodiimide agent, or aldehyde containing ligands in the presence of a reducing agent. Amine-activated SepFast is supplied as an aqueous suspension in 20% ethanol.

Group to be coupled

-COOH, CHO

Matrix

Highly cross-linked 4% agarose beads (4HF)

Particle size

50 – 150 µm

Particle size

50 – 150 µm

Spacer arm

9 atoms

Activation level

10 - 25 µmol amine / ml medium

pH stability

2 -13 (ligand dependent)

Chemical stability

Compatible with all commonly used aqueous chemicals, provided the ligand to be coupled can withstand

Storage

+4℃—+8

Product Series:

Product

                  Quantity

 Code no.

Amine-activated SepFast 4HF

                  5 ml                     

350101-5ML


                  50 ml

350101-50ML


                  1 Litre

350101-1L

Amine-activated SepFast 6HF

                  5 ml

350301-5ML


                  50 ml

350301-50ML


                  1 Litre

350301-1L


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