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NHS活化瓊脂糖層析填料
Product Introduction:
NHS-activated SepFast media forms chemically stable amide bonds with ligands containing primary amine groups. The activated NHS group has a long spacer arm (11 atoms) that is particularly helpful in the immobilization of structurally restricted ligands such as proteins and peptides. This pre-activated agarose base matrix can be readily employed to make various custom affinity chromatography adsorbents for both small scale and large scale purification applications.
Product Properties:
NHS activated SepFast 4HF is made of highly cross-linked 4% beaded agarose. It shows high mechanical rigidity allowing high flow throughput with reduced back pressure in a packed column.
Agarose has long been used for chromatographic separations due to its excellent hydrophilic and low non-specific-binding nature. The particles have an open pore structure with excellent mass transfer properties to various molecules.
The base matrix is activated through a long hydrophilic spacer arm (11 atoms) with N-hydroxysuccinimide at the end. It reacts directly with the primary amine groups in molecules to be immobilized to form stable amide bonds. NHS-activated SepFast media is supplied as a suspension in isopropanol.
Product Series:
Product | Quantity | Code no. |
NHS-activated SepFast 4HF | 5 ml | 330101-5ML |
50 ml | 330101-50ML | |
1 Litre | 330101-1L |
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