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Normal Human Glomerular Endothelial Cells

人腎小球內(nèi)皮細(xì)胞

Normal Human Glomerular Endothelial Cells (NhGEC) are specialized microvascular cells that make up the innermost layer of the glomerular filtration barrier. They are separated from podocytes by a thin layer of glomerular basement membrane. These cells exhibit cobblestone morphology at confluence and present fenestrations specific of this cell type.

The NhGEC are obtained after cortex digestion, and single glomerular isolation. Glomeruli are further digested and then NhGEC are isolated by CD31 positive selection as a pure population.

The NhGEC express factor VIII-related antigen, angiotensin converting enzyme activity, and endocytosed acetylated low-density lipoproteins. They are also characterized by the presence of intracellular Weibel-Palade bodies and caveolae and microvillous projections on the luminal surface.

The NhGEC will be evaluated for the expression of endothelial specific markers (e.g. CD31, vWF and Ve-Cadherin) by  immunofluorescence.

NhGEC can be expanded between 5 to 8 passages, while maintaining their particular characteristics.

NhGEC are terminally differentiated cells and present no significant differentiation capacity.

The NhGEC are for research use only and not approved for human use, or for in vitro diagnostic testing.

Normal Human Glomerular Endothelial Cells

人腎小球內(nèi)皮細(xì)胞

PBMC分離試劑盒

人外周血單個核細(xì)胞分離試劑盒PBMC分離液

人PBMC分離試劑盒 人PBMC分離液

猴外周血單個核細(xì)胞分離試劑盒PBMC分離液

猴PBMC分離試劑盒 猴PBMC分離液

比格犬外周血單個核細(xì)胞分離試劑盒PBMC分離液

比格犬PBMC分離試劑盒 比格犬PBMC分離液

狗外周血單個核細(xì)胞分離試劑盒PBMC分離液

狗PBMC分離試劑盒 狗PBMC分離液

大鼠外周血單個核細(xì)胞分離試劑盒PBMC分離液

大鼠PBMC分離試劑盒 大鼠PBMC分離液

小鼠外周血單個核細(xì)胞分離試劑盒PBMC分離液

小鼠PBMC分離試劑盒 小鼠PBMC分離液

兔外周血單個核細(xì)胞分離試劑盒PBMC分離液

兔PBMC分離試劑盒 兔PBMC分離液

豬外周血單個核細(xì)胞分離試劑盒PBMC分離液

豬PBMC分離試劑盒 豬PBMC分離液

豚鼠外周血單個核細(xì)胞分離試劑盒PBMC分離液

豚鼠PBMC分離試劑盒 豚鼠PBMC分離液

骨髓單個核細(xì)胞分離試劑盒Bone Marrow Mononuclear Cells Isolation Kit

脾單個核細(xì)胞分離試劑盒 Spleen Mononuclear Cells Isolation Kit

紅細(xì)胞分離試劑盒

人紅細(xì)胞分離試劑盒 紅細(xì)胞分離液

猴紅細(xì)胞分離試劑盒 紅細(xì)胞分離液

比格犬紅細(xì)胞分離試劑盒 紅細(xì)胞分離液

大鼠紅細(xì)胞分離試劑盒 紅細(xì)胞分離液

小鼠紅細(xì)胞分離試劑盒 紅細(xì)胞分離液

ADME相關(guān)產(chǎn)品

Ⅰ相代謝穩(wěn)定性試劑盒

Ⅱ相代謝穩(wěn)定性試劑盒

CYP450 酶代謝表型研究試劑盒（化學(xué)抑制法/7種抑制劑）

CYP450 酶代謝表型研究試劑盒（重組酶法/7種酶）

CYP450 酶代謝表型研究試劑盒（重組酶法/單酶）

酶抑制（IC50）研究試劑盒（7種特異性底物）

酶抑制（IC50）研究試劑盒（單個酶）

NADPH再生系統(tǒng)

UGT孵育系統(tǒng)

0.1M PBS

肝微粒體（人，猴，犬，大鼠，小鼠）

肝S9（人，猴，犬，大鼠，小鼠）

肝原代細(xì)胞（人，猴，犬，大鼠，小鼠）

CYP450（CYP1A2，CYP2A6，CYP2B6，CYP2C8，CYP2C9，CYP2C19，CYP2D6，CYP2E1，CYP3A4）

UGT酶

探針底物，代謝產(chǎn)物，抑制劑（CYP1A2，CYP2A6，CYP2B6，CYP2C8，CYP2C9，CYP2C19，CYP2D6，CYP2E1，CYP3A4）

ADME服務(wù)項目

肝微粒體代謝穩(wěn)定性（人，猴，犬，大鼠，小鼠）

肝細(xì)胞代謝穩(wěn)定性試驗（人，猴，犬，大鼠，小鼠）

代謝表型研究

代謝產(chǎn)物鑒定

代謝途徑鑒定

種屬比較研究

CYPP450抑制實驗（CYP1A2，CYP2A6，CYP2C9，CYP2C19，CYP2D6，CYP2E1，CYP3A4）

CYPP450誘導(dǎo)實驗

血漿蛋白結(jié)合率測定

血漿穩(wěn)定性試驗

跨膜轉(zhuǎn)運試驗

藥物-藥物相互作用

毒理學(xué)研究

Innovative Reagents for Innovative Research

匯智和源致力于為創(chuàng)新藥企業(yè)及生命科學(xué)研究機構(gòu)提供高品質(zhì)的生物試劑。